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American Journal of Potato Research

, Volume 96, Issue 6, pp 614–616 | Cite as

Complete Genome Sequence of Phytopathogenic Pectobacterium atrosepticum Lytic Bacteriophage Peat2

  • Melanie Kalischuk
  • John Hachey
  • Dallas Thomas
  • Dan Johnson
  • Lawrence KawchukEmail author
Short Communication
  • 27 Downloads

Abstract

Pectobacterium atrosepticum is a pectolytic enterobacterium that causes blackleg in plants. Bacteriophage Peat2 was isolated from P. atrosepticum infected potato tissue and its genome sequenced. Analysis of the 48,659 bp genome revealed that Peat2 resembles the Myoviridae family Klebsiella pneumoniae bacteriophage JD001 and includes a CRISPR Cas 4 RecB-like nuclease. A lytic bacteriophage, Peat2 details provide important information for application as a biocontrol agent of disease caused by phytopathogenic P. atrosepticum.

Keywords

Blackleg Pectobacterium atrosepticum Bacteriophage Myoviridae Biocontrol Cas 4 

Resumen

Pectobacterium atrosepticum es una enterobacteria pectolítica que causa la pierna negra en plantas. Se aisló el Bacteriofago Peat2 de tejido de papa infectado con P. atrosepticum y se secuenció su genoma. El análisis del genoma de 48,659 pb reveló que Peat2 se asemeja al bacteriófago JD001 de Klebsiella pneumonia de la familia Myoviridae e incluye una nucleasa CRISPR Cas 4 tipo RecB. Los detalles del bacteiofago lítico Peat2 proporcionan información importante para su aplicación como un agente de biocontrol de la enfermedad causada por la fitopatogénica P. atrosepticum.

Notes

Acknowledgments

We thank Dr. F. Leggett for assistance with the transmission electron photomicrographs. Financial support for this work was provided in part by the Alberta Crop Industry Development Fund (ACIDF) grant 2014C008R and AAFC project J002220.

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Copyright information

© The Potato Association of America 2019

Authors and Affiliations

  1. 1.Agriculture and Agri-Food CanadaLethbridgeCanada
  2. 2.Department of GeographyUniversity of LethbridgeLethbridgeCanada

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