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American Journal of Potato Research

, Volume 95, Issue 6, pp 650–658 | Cite as

Optimization of Hairy Root Induction in Solanum tuberosum

  • Moehninsi
  • Duroy A. Navarre
Article

Abstract

An efficient protocol for hairy root induction in Solanum tuberosum was established using Agrobacterium rhizogenes. Transformation of Desiree was more efficient than with Shepody or Purple Majesty. Transformation efficiency of Desiree tuber discs (97% transformation efficiency) was higher in comparison to root (67% transformation efficiency), stem (61% transformation efficiency) and leaf (14% transformation efficiency) explants. Bacterial density, culture parameters, explant type, and genotype influenced the transformation efficiency, as well as the growth of hairy roots. Hairy root transformation efficiency in explants treated with acetosyringone was enhanced from 72 to 89% in Purple Majesty tubers and 56 to 80% in roots during co-cultivation. The growth rate of hairy roots from tuber discs was about 5 times greater than that of hairy roots from leaves. Tuber hairy roots were highly resistant to kanamycin in the absence of NPT II. This study describes the efficient generation of hairy roots in different potato tissues and cultivars that can provide a fast method to generate transformed tissue or be used for production of secondary metabolites.

Keywords

Potatoes Agrobacterium rhizogenes Hairy roots 

Resumen

Se estableció un protocolo eficiente para la inducción de pelos radicales en Solanum tuberosum usando Agrobacterium rhizogenes. Fue más eficiente la transformación de Desiree que con Shepody o Purple Majesty. La eficiencia en la transformación de explantes de discos de tubérculo de Desiree (97% de eficiencia de la transformación) fue más alta en comparación al de raíz (67% de eficiencia de transformación), al del tallo (61% de eficiencia de transformación) y al de hoja (14% de eficiencia en transformación). La densidad de las bacterias, los parámetros del cultivo, el tipo de explante y el genotipo, influenciaron la eficiencia de la transformación, así como el crecimiento de los pelos radicales. La eficiencia de la transformación a pelos radicales en los explantes tratados con acetosyringone se incrementó de 72% a 89% en tubérculos de Purple Majesty, y de 56% a 80% en raíces durante el co-cultivo. El nivel de crecimiento de pelos radicales de discos de tubérculo fue cerca de cinco veces mayor que el de pelos radicales de hojas. Los de tubérculo fueron altamente resistentes a kanamicina en la ausencia de NPT II. Este estudio describe la eficiente generación de pelos radicales en diferentes tejidos y variedades de papa que pudieran aportar un método rápido para generar tejido transformado o usarse para la producción de metabolitos secundarios.

Notes

Acknowledgements

We thank Joanne Holden for help with the tissue culture and maintenance.

Compliance with Ethical Standards

Disclaimer

The use of trade, firm, or corporation names in this publication (or page) is for the information and convenience of the reader. Such use does not constitute an official endorsement or approval by the United States Department of Agriculture or the Agricultural Research Service of any product or service to the exclusion of others that may be suitable.

Supplementary material

12230_2018_9671_MOESM1_ESM.docx (17 kb)
ESM 1 (DOCX 17 kb)
12230_2018_9671_MOESM2_ESM.pptx (400 kb)
ESM 2 (PPTX 399 kb)

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Copyright information

© The Potato Association of America 2018

Authors and Affiliations

  1. 1.Irrigated Agricultural Research and Extension CenterWashington State UniversityProsserUSA
  2. 2.USDA-Agricultural Research ServiceYakima Agricultural Research LaboratoryProsserUSA

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