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Comparison of Two Strategies to Introgress Genes for Resistance to Common Scab from Diploid Solanum chacoense into Tetraploid Cultivated Potato

  • Shelley Jansky
  • Kathleen Haynes
  • David Douches
Article
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Abstract

Wild potato relatives are important sources of novel genes useful for potato improvement. Since most wild potato species are diploid and most cultivars are tetraploid, it is typically necessary to employ ploidy manipulations to access wild potato germplasm. A common strategy is unilateral sexual polyploidization (USP), in which a tetraploid cultivated clone is crossed with a diploid that produces 2n gametes. The diploid parent may be a wild plant selected for a trait of interest. Alternatively, the wild plant may be crossed to a cultivated diploid and, after selection, the diploid hybrid is then crossed to the tetraploid parent. This study was designed to compare these two strategies for the introgression of common scab resistance from wild diploid Solanum chacoense (chc) into cultivated potato. Progeny were evaluated in naturally scab-infested soil in Maine and Michigan. There were no differences between introgression methods for tuber surface area covered by scab. Scab lesions were significantly more severe when a hybrid was used as the diploid parent, but this difference is likely not large enough to be important in a breeding program. Reciprocal cross differences were not significant. However, location x clone interactions were detected for both surface area and lesion type. This study revealed that, while hybridization strategy did not play a major role in developing this scab resistant germplasm, it is critical to consider production environment when screening for resistance.

Keywords

Unilateral sexual polyploidization 2n gametes Streptomyces scabies Solanum tuberosum Disease resistance breeding 

Resumen

Los parientes silvestres de la papa son fuentes importantes de genes novedosos útiles para el mejoramiento de la papa. Considerando que la mayoría de las especies silvestres de papa son diploides, y la mayoría de las variedades son tetraploides, es necesario típicamente emplear manipulaciones de ploidía para tener acceso al germoplasma de papa silvestre. Una estrategia común es poliploidización sexual unilateral (USP), en la cual un clon tetraploide cultivado se cruza con un diploide que produce gametos 2n. El progenitor diploide puede ser una planta silvestre seleccionada por un carácter de interés. Alternativamente, la planta silvestre pudiera cruzarse a una diploide cultivada, y después de la selección, el híbrido diploide se cruza con el progenitor tetraploide. Este estudio se diseñó para comparar estas dos estrategias para la introgresión de la resistencia a la roña común de la especie silvestre Solanum chacoense (chc) en papa cultivada. Se evaluó la progenie en un suelo infestado de roña de manera natural en Maine y Michigan. No hubo diferencias entre los métodos de la introgresión para área de la superficie del tubérculo cubierta por roña. Las lesiones por roña fueron significativamente más severas cuando se usó un híbrido como el progenitor diploide, pero esta diferencia es probable que no sea lo suficientemente grande para ser importante en un programa de mejoramiento. Las diferencias en cruzas recíprocas no fueron significativas. No obstante, las interacciones localidad x clon se detectaron tanto para área en la superficie como para el tipo de lesión. Este estudio reveló que mientras la estrategia de hibridación no juega un papel mayor en el desarrollo de germoplasma resistente a la roña, es crítico considerar el ambiente de producción cuando se evalúa para resistencia.

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Copyright information

© The Potato Association of America 2019

Authors and Affiliations

  1. 1.USDA-ARSUniversity of Wisconsin-MadisonMadisonUSA
  2. 2.USDA-ARSBeltsvilleUSA
  3. 3.Michigan State UniversityEast LansingUSA

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