American Journal of Potato Research

, Volume 93, Issue 4, pp 303–320 | Cite as

Sequence-Specific Nucleases for Genetic Improvement of Potato



Genome editing using sequence-specific nucleases (SSNs) is rapidly becoming a standard tool for genetic engineering in crop species. The implementation of zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and CRISPR/Cas (clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated systems (Cas)) for inducing double-strand breaks enables targeting of virtually any sequence for genetic modification. Targeted mutagenesis via nonhomologous end-joining (NHEJ) and gene targeting via homologous recombination (HR) have been demonstrated in a number of plant species but reports have been limited in vegetatively propagated crops, such as potato (Solanum tuberosum Group Tuberosum L.). This review provides a historical overview of genetic engineering in agriculture, applications of SSN technologies for genome editing in plant species, and prospects of using SSNs for genetic improvement of potato.


Genome editing TALEN CRISPR/Cas Targeted mutagenesis Gene targeting Homologous recombination 


La edición del genoma utilizando nucleasas de secuencias específicas (SSNs) se está volviendo rápidamente una herramienta común para ingeniería genética en especies de cultivos. La implementación de nucleasas de dedos de zinc (ZFNs), nucleasas de efecto de activador de transcripción (TALENs) y CRISPR/Cas (agrupamientos de repeticiones cortas palindromicas regularmente interespaciadas (CRISPR)/sistemas asociados CRISPR (Cas)) para la inducción de rompimientos de doble cadena, permiten identificar objetivos de virtualmente cualquier secuencia para modificación genética. Se ha demostrado en un número de especies de plantas mutagénesis como objetivo vía unión de extremo no homóloga (NHEJ) y gene-objetivo vía recombinación homóloga (HR), pero los reportes son limitados a cultivos propagados vegetativamente, como la papa (Solanum tuberosum Grupo Tuberosum). Esta revisión proporciona una vista general histórica de ingeniería genética en agricultura, aplicaciones de tecnologías SSN para la edición de genomas en especies vegetales, y las perspectivas del uso SSNs para el mejoramiento genético de la papa.


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Copyright information

© The Potato Association of America 2016

Authors and Affiliations

  1. 1.Department of Plant, Soil, and Microbial SciencesMichigan State UniversityEast LansingUSA

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