American Journal of Potato Research

, Volume 93, Issue 3, pp 203–212 | Cite as

Development of Molecular Markers Closely Linked to the Potato Leafroll Virus Resistance Gene, Rlretb, for use in Marker-Assisted Selection

  • Joseph C. Kuhl
  • Richard G. Novy
  • Jonathan L. Whitworth
  • Margaret S. Dibble
  • Brian Schneider
  • Darren Hall


Potato leafroll virus (PLRV) is a major pathogen of potato with worldwide impact on seed and commercial production of potato. In North America, the primary potato cultivars grown by industry are not resistant to PLRV and require the application of insecticides to control the aphid vector of PLRV, so as to prevent PLRV infection of the crop. The Solanum etuberosum- derived dominant gene Rlretb confers resistance to PLRV and has been mapped to chromosome 4. The previous closest marker to Rlretb, C2_At1g42990, was mapped 13.6 cM from the gene in a BC3 population. Cleaved amplified polymorphic sequence markers were developed in the region surrounding C2_At1g42990 and mapped along with Rlretb in 102 BC4 progeny. Sixteen markers were identified surrounding Rlretb, with flanking markers at 2.1 and 9.3 cM. Two markers, 1367-8a and DMB32-11, both 2.1 cM from Rlretb, are shown to be well suited for marker assisted selection. The development of additional molecular markers more closely linked to Rlretb will greatly facilitate its use in potato breeding and its continued introgression into cultivated potato for the development of PLRV-resistant cultivars.


Potato Solanum tuberosum S. etuberosum Potato leafroll virus Resistance 


El virus del enrollamiento de la hoja de la papa (PLRV) es un patógeno importante de la papa con impacto mundial en la producción de semilla y en la producción comercial de la papa. En Norteamérica, las principales variedades de papa que se cultivan por la industria no son resistentes al PLRV y requieren de la aplicación de insecticidas para control del áfido vector del PLRV, así como para prevenir la infección del cultivo por PLRV. El gen dominante derivado de Solanum etuberosum, Rlretb, confiere resistencia al PLRV y ha sido ubicado en el cromosoma 4. El marcador previo más cercano al Rlretb, C2_At1g42990, fue ubicado a 13.6 cM del gen en una población BC3. Se desarrollaron marcadores fragmentados amplificados de secuencias polimórficas en la región que rodea a C2_At1g42990 y se les hizo un mapa junto con Rlretb en una progenie 102 BC4. Se identificaron 16 marcadores rodeando al Rlretb, con marcadores en los lados a 2.1 y 9.3 cM. Dos marcadores, 1367-8a y DMB32-11, ambos a 2.1 cM de Rlretb, se ha demostrado que son los apropiados para la selección asistida por marcadores. El desarrollo de marcadores moleculares adicionales más cercanamente ligados a Rlretb facilitará grandemente su uso en el mejoramiento de la papa así como su incorporación a papa cultivada para el desarrollo de variedades resistentes al PLRV.

Supplementary material

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Supplemental Figure 1(DOCX 230 kb)


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Copyright information

© The Potato Association of America 2016

Authors and Affiliations

  • Joseph C. Kuhl
    • 1
  • Richard G. Novy
    • 2
  • Jonathan L. Whitworth
    • 2
  • Margaret S. Dibble
    • 1
  • Brian Schneider
    • 2
  • Darren Hall
    • 2
  1. 1.Department of Plant, Soil, and Entomological SciencesUniversity of IdahoMoscowUSA
  2. 2.USDA/ARS, Small Grains and Potato Germplasm Research UnitAberdeenUSA

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