American Journal of Potato Research

, Volume 93, Issue 3, pp 203–212 | Cite as

Development of Molecular Markers Closely Linked to the Potato Leafroll Virus Resistance Gene, Rlretb, for use in Marker-Assisted Selection

  • Joseph C. Kuhl
  • Richard G. Novy
  • Jonathan L. Whitworth
  • Margaret S. Dibble
  • Brian Schneider
  • Darren Hall
Article

Abstract

Potato leafroll virus (PLRV) is a major pathogen of potato with worldwide impact on seed and commercial production of potato. In North America, the primary potato cultivars grown by industry are not resistant to PLRV and require the application of insecticides to control the aphid vector of PLRV, so as to prevent PLRV infection of the crop. The Solanum etuberosum- derived dominant gene Rlretb confers resistance to PLRV and has been mapped to chromosome 4. The previous closest marker to Rlretb, C2_At1g42990, was mapped 13.6 cM from the gene in a BC3 population. Cleaved amplified polymorphic sequence markers were developed in the region surrounding C2_At1g42990 and mapped along with Rlretb in 102 BC4 progeny. Sixteen markers were identified surrounding Rlretb, with flanking markers at 2.1 and 9.3 cM. Two markers, 1367-8a and DMB32-11, both 2.1 cM from Rlretb, are shown to be well suited for marker assisted selection. The development of additional molecular markers more closely linked to Rlretb will greatly facilitate its use in potato breeding and its continued introgression into cultivated potato for the development of PLRV-resistant cultivars.

Keywords

Potato Solanum tuberosum S. etuberosum Potato leafroll virus Resistance 

Resumen

El virus del enrollamiento de la hoja de la papa (PLRV) es un patógeno importante de la papa con impacto mundial en la producción de semilla y en la producción comercial de la papa. En Norteamérica, las principales variedades de papa que se cultivan por la industria no son resistentes al PLRV y requieren de la aplicación de insecticidas para control del áfido vector del PLRV, así como para prevenir la infección del cultivo por PLRV. El gen dominante derivado de Solanum etuberosum, Rlretb, confiere resistencia al PLRV y ha sido ubicado en el cromosoma 4. El marcador previo más cercano al Rlretb, C2_At1g42990, fue ubicado a 13.6 cM del gen en una población BC3. Se desarrollaron marcadores fragmentados amplificados de secuencias polimórficas en la región que rodea a C2_At1g42990 y se les hizo un mapa junto con Rlretb en una progenie 102 BC4. Se identificaron 16 marcadores rodeando al Rlretb, con marcadores en los lados a 2.1 y 9.3 cM. Dos marcadores, 1367-8a y DMB32-11, ambos a 2.1 cM de Rlretb, se ha demostrado que son los apropiados para la selección asistida por marcadores. El desarrollo de marcadores moleculares adicionales más cercanamente ligados a Rlretb facilitará grandemente su uso en el mejoramiento de la papa así como su incorporación a papa cultivada para el desarrollo de variedades resistentes al PLRV.

Supplementary material

12230_2016_9496_MOESM1_ESM.docx (230 kb)
Supplemental Figure 1(DOCX 230 kb)

References

  1. Ahmadvand R., P. Poczai, R. Hajianfar, B. Kolics, A.M. Gorji, Z. Polgár, and J. Taller. 2014. Next generation sequencing based development of intron-targeting markers in tetraploid potato and their transferability to other Solanum species. Gene 540: 117–121.CrossRefPubMedGoogle Scholar
  2. Ahn Y.K., J.W. Bang, G.S. Choi, and H.G. Park. 2001. Introgression of resistance to PLRV and PVY from solanum brevidens into S. tuberosum (‘superior’, ‘Dejima’, and dihaploid of ‘superior’) by interspecific somatic hybrids. Journal of the Korean Society for Horticultural Science 42: 707–711.Google Scholar
  3. Alseekh S., I. Ofner, T. Pleban, P. Tripodi, F.D. Dato, M. Cammareri, A. Mohammad, S. Grandillo, A.R. Fernie, and D. Zamir. 2013. Resolution by recombination: breaking up Solanum pennellii introgressions. Trends in Plant Science 18: 536–538.CrossRefPubMedGoogle Scholar
  4. Brown C.R., and P.E. Thomas. 1994. Resistance to potato leafroll virus derived from Solanum chacoense: characterization and inheritance. Euphytica 74: 51–57.CrossRefGoogle Scholar
  5. Carrasco A., J.I. Ruiz de Galarreta, A. Rico, and E. Ritter. 2000. Transfer of PLRV resistance from Solanum verrucosum schlechdt to potato (S. tuberosum L.) by protoplast electrofusion. Potato Research 43: 31–42.CrossRefGoogle Scholar
  6. Chanter D.O. 1975. Modifications of the angular transformation. J. R statistic Soc. Series C (Applied Statistics) 24: 354–359.Google Scholar
  7. Corsini D.L., J.J. Pavek, M.W. Martin, and C.R. Brown. 1994. Potato germplasm with combined resistance to leafroll virus and viruses X and Y. American Potato Journal 71: 377–385.CrossRefGoogle Scholar
  8. Felcher K.J., J.J. Coombs, A.N. Massa, C.N. Hansey, J.P. Hamilton, R.E. Veilleux, C.R. Buell, and D.S. Douches. 2012. Integration of two diploid potato linkage maps with the potato genome sequence. PloS One 7: e36347.CrossRefPubMedPubMedCentralGoogle Scholar
  9. Flis B., and I. Wasilewicz-Flis. 1998. Progeny tests to identify diploid potato clones homozygous at loci controlling resistance to PLRV. Potato Research 41: 219–228.CrossRefGoogle Scholar
  10. Gavrilenko T., R. Thieme, U. Heimback, and T. Thieme. 2003. Fertile somatic hybrids of Solanum etuberosum (+) dihaploid Solanum tuberosum and their backcrossing progenies: relationships of genome dosage with tuber development and resistance to potato virus Y. Euphytica 131: 323–332.CrossRefGoogle Scholar
  11. Gillen A.M., and R.G. Novy. 2007. Molecular characterization of the progeny of Solanum etuberosum identifies a genomic region associated with resistance to potato leafroll virus. Euphytica 155: 403–415.CrossRefGoogle Scholar
  12. Hirsch C.D., J.P. Hamilton, K.L. Childs, J. Cepela, E. Crisovan, B. Vaillancourt, C.N. Hirsch, M. Habermann, B. Neal, and C.R. Buell. 2014. Spud DB: a resource for mining sequences, genotypes, and phenotypes to accelerate potato breeding. Plant Genome 7: 1–12.CrossRefGoogle Scholar
  13. Jansky, S.H. 2000. Breeding for disease resistance in potato. In: Plant breeding reviews, ed. J. Janick, 69–155. New York: Wiley.Google Scholar
  14. Kelley K.B., J.L. Whitworth, and R.G. Novy. 2009. Mapping of the potato leafroll and virus resistance gene, Rlr etb, from Solanum etuberosum identifies interchromosomeal translocations among its E-genome chromsomes 4 and 9 relative to the a-genome of solanum L. sect. Petota. Molecular Breeding 23: 489–500.CrossRefGoogle Scholar
  15. Manly M.F. 1993. A Macintosh program for storage and analysis of experimental genetic mapping data. Mammalian Genome 4: 303–313.CrossRefPubMedGoogle Scholar
  16. Manly K.R., and R.H. Cudmore. 1998. Map manager XP. San Diego: In Abstracts of Plant and Animal Genome VI.Google Scholar
  17. Marczewski W., B. Flis, J. Syller, R. Schäfer-Pregl, and C. Gebhardt. 2001. A major quantitative trait locus for resistance to potato leafroll virus is located in a resistance hotspot on potato chromosome XI and is tightly linked to N-gene-like markers. Molecular Plant-Microbe Interactions 14: 1420–1425.CrossRefPubMedGoogle Scholar
  18. Marczewski W., B. Flis, J. Syller, D. Strzelczyk-Żyta, J. Hennig, and C. Gebhardt. 2004. Two allelic or tightly linked genetic factors at the PLRV.4 locus on potato chromosome XI control resistance to potato leafroll virus accumulation. Theoretical Applied Genetics 109: 1604–1609.CrossRefPubMedGoogle Scholar
  19. Matsubayashi, M. 1991. Phylogenetic relationships in the potato and its related species. In: Chromosome engineering in plants: genetics, breeding, evolution part B, ed. T. Tsuchiya and P.K. Gupta. 93–118. Netherlands: Elsevier.Google Scholar
  20. McGrath J.M., S.M. Wielgus, T.F. Uchytil, H. Kim-Lee, G.T. Haberlach, C.E. Williams, and J.P. Helgeson. 1994. Recombination of Solanum brevidens chromosomes in the second backcross generation from a somatic hybrid with S. tuberosum. Theoretical and Applied Genetics 88: 917–924.CrossRefPubMedGoogle Scholar
  21. Mihovilovich E., L. Alarcón, A.L. Pérez, J. Alvarado, C. Arellano, and M. Bonierbale. 2007. High levels of heritable resistance to potato leafroll virus (PLRV) in Solanum tuberosum subsp. andigena. Crop Science 47: 1091–1103.CrossRefGoogle Scholar
  22. Mihovilovich E., M. Aponte, H. Lindqvist-Kreuze, and M. Bonierbale. 2014. An RGA-derived SCAR marker linked to PLRV resistance from Solanum tuberosum ssp. andigena. Plant Molecular Biology Reporter 32: 117–128.CrossRefGoogle Scholar
  23. Mowry T.M. 2005. Insecticidal reduction of Potato leafroll virus transmission by Myzus persicae. Annals of Applied Biology 146: 81–88.CrossRefGoogle Scholar
  24. Novy R.G., and J.P. Helgeson. 1994. Somatic hybrids between Solanum etuberosum and diploid, tuber bearing Solanum clones. Theoretical and Applied Genetics 89: 775–782.PubMedGoogle Scholar
  25. Novy R.G., A. Nasruddin, D.W. Ragsdale, and E.B. Radcliffe. 2002. Genetic resistances to potato leafroll virus, potato virus Y, and green peach aphid in progeny of Solanum etuberosum. American Journal of Potato Research 79: 9–18.CrossRefGoogle Scholar
  26. Novy R.G., A.M. Gillen, and J.L. Whitworth. 2007. Characterization of the expression and inheritance of potato leafroll virus (PLRV) and potato virus Y (PVY) resistance in three generations of germplasm derived from Solanum etuberosum. Theoretical and Applied Genetics 114: 1161–1172.CrossRefPubMedGoogle Scholar
  27. Parokonny A.S., J.A. Marshall, M.D. Bennett, E.C. Cocking, M.R. Davey, and J.B. Power. 1997. Homoeologous pairing and recombination in backcross derivatives of tomato somatic hybrids [Lycopersicon esculentum (+) L. peruvianum]. Theoretical and Applied Genetics 94: 713–723.CrossRefGoogle Scholar
  28. Perez F., A. Menendez, P. Dehal, and C.F. Quiros. 1999. Genomic structural differentiation in solanum: comparative mapping of the a- and E-genomes. Theoretical and Applied Genetics 98: 1183–1193.CrossRefGoogle Scholar
  29. PGSC. 2011. Genome sequence and analysis of the tuber crop potato. Nature 475: 189–197.CrossRefGoogle Scholar
  30. Poczai P., I. Cernák, A.M. Gorji, S. Nagy, J. Taller, and Z. Polgár. 2010. Development of intron targeting (IT) markers for potato and cross-species amplification in Solanum nigrum (Solanaceae). American Journal of Botany 97: e142–e145.CrossRefPubMedGoogle Scholar
  31. Ramanna M.S., and J.G.T.H. Hermsen. 1981. Structural hybridity in the series etuberosa of the genus solanum and its bearing on crossability. Euphytica 30: 15–31.CrossRefGoogle Scholar
  32. Solomon-Blackburn R.M., and H. Barker. 2001. Breeding virus resistant potatoes (Solanum tuberosum): a review of traditional and molecular approaches. Heredity 86: 17–35.CrossRefPubMedGoogle Scholar
  33. Spooner D.M., and R. Hijmans. 2001. Potato systematics and germplasm collecting, 1989–2000. American Journal of Potato Research 78: 237–268.CrossRefGoogle Scholar
  34. Swiezynski K.M., M.A. Dziewonska, and K. Ostrowska. 1989. Resistance to the potato leafroll virus (PLRV) in diploid potatoes. Plant Breeding 103: 221–227.CrossRefGoogle Scholar
  35. Syller J. 1996. Potato leafroll virus (PLRV): its transmission and control. Integrated Pest Management Reviews 1: 217–227.CrossRefGoogle Scholar
  36. Taliansky M., M.A. Mayo, and H. Barker. 2003. Potato leafroll virus: a classic pathogen shows some new tricks. Molecular Plant Pathology 4: 81–89.CrossRefPubMedGoogle Scholar
  37. Tiwari J.K., D. Poonam, S.K. Sarkar, J. Gopal Pandey, and S.R. Kumar. 2010. Molecular and morphological characterization of somatic hybrids between Solanum tuberosum L. and S. etuberosum lindl. Plant cell. Tissue and Organ Culture 103: 175–187.CrossRefGoogle Scholar
  38. USDA, ARS, National Genetic Resources Program 2014. Germplasm Resources Information Network - (GRIN). [Online Database] National Germplasm Resources Laboratory, Beltsville, Maryland. Available: http://www.ars-grin.gov/cgi-bin/npgs/html/obs.pl?1189587.
  39. Valkonen J.P.T., G. Brigneti, and E. Pehu. 1992a. Resistance to Myzus persicae (Sulz.) in wild potatoes of the series Etuberosa. Acta Agriculturu Scandinavica. Section B. Soil and Plant Science 42: 118–127.Google Scholar
  40. Valkonen J.P.T., G. Brigneti, L.F. Salazar, E. Pehu, and R.W. Gibson. 1992b. Interactions of the solanum spp. of the etuberosa group and nine potato-infecting viruses and a viroid. Annals of Applied Biology 120: 301–313.CrossRefGoogle Scholar
  41. Velásquez A.C., E. Mihovilovich, and M. Bonierbale. 2007. Genetic characterization and mapping of major gene resistance to potato leafroll virus in Solanum tuberosum ssp andigena. Theoretical Applied Genetics 114: 1051–1058.CrossRefPubMedGoogle Scholar

Copyright information

© The Potato Association of America 2016

Authors and Affiliations

  • Joseph C. Kuhl
    • 1
  • Richard G. Novy
    • 2
  • Jonathan L. Whitworth
    • 2
  • Margaret S. Dibble
    • 1
  • Brian Schneider
    • 2
  • Darren Hall
    • 2
  1. 1.Department of Plant, Soil, and Entomological SciencesUniversity of IdahoMoscowUSA
  2. 2.USDA/ARS, Small Grains and Potato Germplasm Research UnitAberdeenUSA

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