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American Journal of Potato Research

, Volume 86, Issue 3, pp 227–238 | Cite as

Comparison of Genome Sequence of PVY Isolates with Biological Properties

  • Hugh Barker
  • Kara D. McGeachy
  • Nataša Toplak
  • Kristina Gruden
  • Jana Žel
  • Isla Browning
Article

Abstract

The whole RNA genomes of six European Potato virus Y (PVY) isolates, representing isolates of the PVYO, PVYN and PVYNTN subgroups, were sequenced and the reactions of a range of tobacco and potato cultivars after the inoculation were determined. One isolate induced symptoms of veinal necrosis when inoculated to tobacco, but reacted with PVYO- specific antibodies. The genome sequence of this isolate showed that it had a recombinant genome comprising large sections of sequence that are characteristic of N and O sub-group isolates. Another isolate caused mosaic symptoms when inoculated to tobacco, and reacted with PVYN- specific antibodies. The nucleotide sequence of its genome was very closely related to the genome sequence published for two potato-infecting PVY isolates from North America. These three isolates are the members of interesting group NA (North American). They have highly similar genome sequences throughout their length, but biologically represent isolates from the PVYO, PVYN and PVYNTN subgroups. The occurrence of these biologically diverse isolates with their highly homologous genomes brings into question the exact nature of gene structure in relation to function.

Keywords

Potato virus PVY isolates Tobacco Potato Sequence Genome 

Resumen

Se secuenciaron los genomas completos de ARN de seis aislamientos europeos del virus Y de la papa (PVY) representantes de aislamientos de los subgrupos de PVYO, PVYN y PVYNTN y se determinaron las reacciones de una serie de cultivares de tabaco y papa después de las inoculaciones. Un aislamiento indujo síntomas de necrosis de las nervaduras cuando fue inoculado en tabaco pero reaccionó con los anticuerpos específicos de PVYO. La secuencia del genoma de este aislamiento mostró que tenía un genoma recombinante con grandes secciones de secuencias que son características de aislamientos de los subgrupos N y O. Otro aislamiento causó síntomas de mosaico cuando se inoculó a tabaco y reaccionó con anticuerpos específicos de PVYN. La secuencia de nucleótidos de su genoma fue relacionada muy cercanamente con la secuencia publicada de dos aislamientos de PVY de Norteamérica que infectan papa. Estos tres aislamientos son miembros del grupo NA (Norte Americano). Ellos tienen una secuencia de genoma altamente similar, pero biológicamente representan aislamientos de los subgrupos PVYO, PVYN y PVYNTN. La presencia de estos aislamientos biológicamente diferentes con sus genomas altamente homólogos, pone en cuestión la naturaleza exacta del gen con relación a su función.

Notes

Acknowledgements

We would like to thank dr. Maja Ravnikar for discussions during our work.

This research was supported partly by a grant from Slovenian Research Agency and British Council as the part of bilateral project P4 165.

Supplementary material

12230_2009_9076_MOESM1_ESM.doc (76 kb)
Supplement 1 Nucleotide sequence maps of complete PVY genomes showing main sequence types and recombination points (DOC 76 kb)
12230_2009_9076_MOESM2_ESM.doc (70 kb)
Supplement 2 Amino acid sequence maps of complete PVY genomes showing main sequence types and recombination points (DOC 69 kb)
12230_2009_9076_MOESM3_ESM.doc (32 kb)
Supplement 3 The number of amino acid differences betwen PVY-SASA-61, PVY-Tu 660 and PVY-N-Jg and on gene-by-gene basis (DOC 32 kb)

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Copyright information

© Potato Association of America 2009

Authors and Affiliations

  • Hugh Barker
    • 1
  • Kara D. McGeachy
    • 1
  • Nataša Toplak
    • 2
    • 4
  • Kristina Gruden
    • 2
  • Jana Žel
    • 2
  • Isla Browning
    • 3
  1. 1.Scottish Crop Research InstituteInvergowrieUK
  2. 2.National Institute of BiologyLjubljanaSlovenia
  3. 3.Scottish Agricultural Science AgencyEdinburghUK
  4. 4.Omega d.o.o.LjubljanaSlovenia

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