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Kew Bulletin

, Volume 65, Issue 1, pp 3–20 | Cite as

Elliptic Fourier Analysis of leaf outline shape in forest fragment populations of Anthurium sinuatum and A. pentaphyllum (Araceae) from Northeast Brazil

  • I. M. Andrade
  • S. J. MayoEmail author
  • D. Kirkup
  • C. Van den Berg
Article

Summary

A field-based comparative study of leaflet outline shape in five populations of Anthurium sinuatum Benth. ex Schott from Amazonia and humid brejo forests of Ceará, Northeast Brazil, and ten populations of A. pentaphyllum (Aubl.) G. Don var. pentaphyllum from Amazonia and the Brazilian Atlantic forest is reported. 1,120 leaflets from 561 individuals in 15 populations were sampled. Using images, leaflet outlines were captured by digitisation and subjected to Elliptic Fourier Analysis (EFA) to produce matrices of Fourier coefficients based on 40 harmonics; lateral and central leaflets were analysed separately. Twelve shape variables, extracted from the Fourier coefficient matrices, described the leaflet outlines of 531 individuals. Between-population relationships were estimated using cluster analysis, Canonical Variates Analysis and non-parametric MANOVA. Amazonian populations showed the greatest within-population variability. In A. sinuatum the Pacatuba population was the most distinct in Ceará and the Pará population (Amazonia) was not significantly different from those at Ibiapaba and Maranguape (both Ceará). In A. pentaphyllum 80% of population pairs were significantly different and three significantly different groups of populations were observed. Although the populations of A. pentaphyllum displayed considerable variation, the morphological patterns did not correlate with previously reported molecular ones. The two species were found to be slightly but significantly different on leaf outline data.

Keywords

Atlantic forest brejo forest Ceará morphological variability morphometrics population differentiation species differentiation 

Notes

Acknowledgements

We are most grateful to the following organisations and persons for their essential help: FUNCAP (Fundação Cearense de Amparo à Pesquisa), FAPESB (Fundação de Amparo à Pesquisa do Estado da Bahia), WWF Brasil, Kew Latin America Research Fellowships Programme (Royal Botanic Gardens, Kew), Margaret Mee Fellowships Programme (Fundação Botânica Margaret Mee – Rio de Janeiro, Royal Botanic Gardens, Kew), Ministério do Meio Ambiente (IBAMA, permit number 001/2004, 042/04), Conselho Nacional de Desenvolvimento Científica e Tecnológica (CNPq, MCT permit number, 168/01), Dr Ana Maria Giulietti, Dr Robyn Cowan, Anna Haigh, Dr Jean-Jacques de Granville and the staff of the CAY herbarium in French Guiana, Bertrand Goguillon and the staff of IRD (French Guiana) for permission to collect, Dr Denis Barabé, Thales Alves Ribeiro, Sr José Teodoro Soares, Rector of the Universidade Estadual do Vale do Acaraú (UVA), Sobral, Ceará, Brazil, Universidade Estadual de Feira de Santana (UEFS), Bahia, Brazil.

References

  1. Anderson, M. J. (2001). A new method for non-parametric multivariate analysis of variance. Austral Ecol. 26: 32 – 46.Google Scholar
  2. Andrade, I. M. (2006). Estudos morfométricos e genéticos em populações de duas espécies de Araceae em áreas florestais do Brasil, especialmente no Ceará. Unpublished PhD thesis, Universidade Estadual de Feira de Santana, Programa de Pós-Graduação em Botânica, Feira de Santana, Bahia, Brazil.Google Scholar
  3. ____, Mayo, S. J., Van den Berg, C., Fay, M. F., Chester, M., Lexer, C. & Kirkup, D. (2007). A preliminary study of genetic variation in populations of Monstera adansonii var. klotzschiana (Araceae) from North-east Brazil, estimated with AFLP molecular markers. Ann. Bot. 100: 1143 – 1154.Google Scholar
  4. ____, ____, Kirkup, D. & Van den Berg, C. (2008). Comparative morphology of populations of Monstera (Araceae) from natural forest fragments in Northeast Brazil using elliptic Fourier Analysis of leaf outlines. Kew Bull. 63: 193 – 211.Google Scholar
  5. ____, ____, Van den Berg, C., Fay, M. F., Chester, M., Lexer, C. & Kirkup, D. (2009). Genetic variation in natural populations of Anthurium sinuatum and A. pentaphyllum var. pentaphyllum (Araceae) from north-east Brazil using AFLP molecular markers. Bot. J. Linn. Soc. 159: 88 – 105.Google Scholar
  6. Barbosa, M. R. V., Agra, M. F., Sampaio, E. V. S. B., Cunha, J. P. & Andrade, L. A. (2004). Diversidade florística da Mata de Pau Ferro, Areia, Paraíba. Pp. 111 – 122. In: K. C. Pôrto, J. J. P. Cabral & M. Tabarelli, (2004), Brejos de altitude em Pernambuco e Paraíba: história natural, ecologia e conservação. Ministério do Meio Ambiente, Brasília.Google Scholar
  7. Cannon, C. H. & Manos, P. S. (2001). Combining and comparing morphometric shape descriptors with a molecular phylogeny: the case of fruit type evolution in Bornean Lithocarpus (Fagaceae). Syst. Biol. 50: 860 – 880.Google Scholar
  8. Croat, T. B. & Sheffer, R. D. (1983). The sectional groupings of Anthurium (Araceae). Aroideana 6: 85 – 123.Google Scholar
  9. Gimp (2006). GNU Image Manipulation Program. http://www.gimp.org. Version 2.2.4 by S. Kimball & P. Mattis.
  10. Hammer, Ø., Harper, D. A. T. & Ryan, P. D. (2001). PAST: Palaeontological Statistics software package for education and data analysis. Palaeontologia Electronica 4(1): 9 pp. http://folk.uio.no/ohammer/past.
  11. Holmgren, P. K. & Holmgren, N. H. (1998). Index Herbariorum: A global directory of public herbaria and associated staff. New York Botanical Garden’s Virtual Herbarium. http://sweetgum.nybg.org/ih/.
  12. Jensen, R. J., Ciofani, K. M. & Miramontes, L. C. (2002). Lines, outlines, and landmarks: morphometric analyses of leaves of Acer rubrum, Acer saccharinum (Aceraceae) and their hybrid. Taxon 51: 475 – 492.Google Scholar
  13. Madison, M. T. (1978). The species of Anthurium with palmately divided leaves. Selbyana 2: 239 – 282.Google Scholar
  14. Mayo, S. J. (1983). Aspectos da fitogeografia das Araceae bahianas. Anais XXXIV Congresso Nacional de Botânica do Brasil, Porto Alegre, Brasil 2: 215 – 227.Google Scholar
  15. Premoli, A. C. (1996). Leaf architecture of South American Nothofagus (Nothofagaceae) using traditional and new methods in morphometrics. Bot. J. Linn. Soc. 121: 25 – 40.Google Scholar
  16. R Development Core Team (2008). R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. http://www.R-project.org.
  17. Raveloson, H., Le Minor, J.-M., Rumpler, Y. & Schmittbuhl, M. (2005). Shape of the lateral mandibular outline in Lemuridae: a quantitative analysis of variability using elliptical Fourier analysis. Folia Primatol. 76: 245 – 261.Google Scholar
  18. Ray, T. S. (1990). Application of eigenshape analysis to second order leaf shape ontogeny in Syngonium podophyllum (Araceae). In: F. J. Rohlf & F. L. Bookstein (eds), Proceedings of the Michigan Morphometrics Workshop, pp. 201 – 213. Special Publication No. 2, The University of Michigan Museum of Zoology, Ann Arbor, Michigan.Google Scholar
  19. ____ (1992). Landmark eigenshape analysis: homologous contours: leaf shape in Syngonium (Araceae). Amer. J. Bot. 79: 69 – 76.Google Scholar
  20. Rohlf, F. J. (2004). TpsDig ver. 1.40. http://life.bio.sunysb.edu/morph/.
  21. ____ (2005). NTSYSpc: Numerical Taxonomy System ver. 2.20d, Exeter Publishing, Ltd. Setauket, NY. http://www.exetersoftware.com/cat/ntsyspc/ntsyspc.html
  22. Rumpunen, K. & Bartish, I. V. (2002). Comparison of differentiation estimates based on morphometric and molecular data, exemplified by various leaf shape descriptors and RAPDs in the genus Chaenomeles (Rosaceae). Taxon 51: 69 – 82.Google Scholar
  23. Slice, D. (1998). Morpheus et al. Multiplatform software for morphometric analysis. http://www.morphometrics.org/id6.html.
  24. Yoshioka, Y., Iwata, H., Ohsawa, R. & Ninomiya, S. (2004). Analysis of petal shape variation of Primula sieboldii by elliptic Fourier descriptors and principal component analysis. Ann. Bot. 94: 657 – 664.Google Scholar

Copyright information

© The Board of Trustees of the Royal Botanic Gardens, Kew 2010

Authors and Affiliations

  • I. M. Andrade
    • 1
  • S. J. Mayo
    • 2
    Email author
  • D. Kirkup
    • 2
  • C. Van den Berg
    • 1
  1. 1.Programa de Pós-Graduação em Botânica, Universidade Estadual de Feira de Santana (UEFS), Departamento de Ciências BiológicasFeira de SantanaBrazil
  2. 2.Herbarium, Library, Art and Archives, Royal Botanic Gardens, KewRichmondUK

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