Folia Geobotanica

, 44:399 | Cite as

Molecular Data Helps Traditional Taxonomy: Re-evaluation of Reseda collina (Resedaceae), and a New Record for Europe



Reseda collina is included in Reseda section Phyteuma, a monophyletic and morphologically well-defined group that is also characterized by taxonomic problems regarding species circumscription. The status of this species has been controversial, usually being subordinated to the widespread R. phyteuma. A study of R. collina and allied taxa based on morphological and molecular (nuclear ITS, plastid trnL-F) data supports the taxonomic identity of R. collina and its separation from R. phyteuma. An epitype is designated to support the holotype and prevent future nomenclature problems. In addition, our molecular and morphological analyses suggest that some problematic southeastern Iberian populations, previously identified as R. phyteuma, belong to R. collina, which implies a new European record for this species that was previously considered as a northwestern African endemic. Following this finding, biogeographic relationships between the southern Iberian Peninsula and northwestern Africa are also discussed.


Arid regions Ibero-North African endemic Molecular systematics Reseda section Phyteuma Taxonomy Typification 



The authors thank M. Míguez and F.J. Fernández for technical support; the curators of ALME, AMD, BM, C, DBN, E, GDA, GH, HUAL, IBF, K, MA, MGC, O, RNG, S, SEV, UPOS and UPS herbaria for the loan of specimens, granting permission for DNA extractions and providing photographs of type material. We are also grateful to M. Escudero, M. Luceño and to two anonymous reviewers for their critical comments on the manuscript. The Royal Botanic Garden of Madrid kindly provided facilities for using the electron microscope. This research was supported by the Spanish Ministry of Science and Technology and the Andalusian Government through the projects CGL2005-06017-C02-02/BOS and P06-RMM-4128, respectively. The experiments performed for this research comply with the current laws of Spain.


  1. Abdallah MS, de Wit HCD (1978) The Resedaceae: a taxonomical revision of the family (final instalment). Meded Landbouwhogeschool Wageningen 78:1–416Google Scholar
  2. Aránega R, Pajarón Sotomayor S (1990) Sobre una Reseda phyteuma L. rupícola. Anales Jard Bot Madrid 41:59–64Google Scholar
  3. Blanca G, Cabezudo B, Hernández-Bermejo E, Herrera CM, Molero Mesa J, Muñoz J, Valdés B (1999) Libro Rojo de la flora silvestre amenazada de Andalucía 1: Especies en peligro de extinción. Consejería de Medio Ambiente, Junta de Andalucía, SevillaGoogle Scholar
  4. Bory MM, Durieu MC (1849) Exploration Scientifique de l´Algérie 3 (Atlas). Imprimiere National, ParisGoogle Scholar
  5. Brummit RK (2001) World geographical scheme for recording plant distributions. Ed 2. Plant Taxonomic Database Standards No. 2, International Working Group on Taxonomic Databases for Plant Sciences (TDWG), Hunt Institute for Botanical Documentation, Carnegie Mellon University, PittsburghGoogle Scholar
  6. Cheek M (1989) On typifying names validated by plates unaccompanied by text. Taxon 38:636–638CrossRefGoogle Scholar
  7. Cueto M, Blanca G, González Rebollar JL (1991) Análisis florístico de las sierras de María y Orce (provincias de Almería y Granada, España). Anales Jard Bot Madrid 48:201–211Google Scholar
  8. Duggen S, Hoernle K, van de Bogaard P, Rüpke L, Phipps Morgan JM (2003) Deep roots on the Messinian salinity crisis. Nature 422:602–606CrossRefPubMedGoogle Scholar
  9. Durand T, Schinz H (1898) Conspectus Florae Africae 1(2). Bruxelles, Berlin, ParisGoogle Scholar
  10. Giménez Luque E, Gómez Mercado F (2002) Análisis de la flora vascular de la Sierra de Gádor (Almería, España). Lazaroa 23:35–43Google Scholar
  11. Guàrdia RG, Allès JV (2008) El herbario de Joan Texidor (1838–1885) en el Centro de Documentación de Biodiversidad Vegetal de Barcelona (BCN). Bol Assoc Herb Íbero-Macaronésicos 10:29–30Google Scholar
  12. Holmgren PK, Holmgren NH, Barnett, LC (1990) Index Herbariorum. Part I: The Herbaria of the world. Ed. 8. Regnum Veg 120:1–693Google Scholar
  13. Hsü KJ, Montardet P, Bernoulli D, Citá MB, Erickson A, Garrison RE, Kidd RB, Mèlierés F, Müller C, Wright R (1977) History of the Mediterranean salinity crisis. Nature 267:399–403CrossRefGoogle Scholar
  14. Ibn Tattou M (1999) Resedaceae. In Fennane M, Ibn Tattou M, Mathez J, Ouyahya A, El Oualidi J (eds) Flora pratique du Maroc 1. Institute Scientifique, Université Mohammed V, Rabat, pp. 440–448Google Scholar
  15. Jafri, SMH (1977) Resedaceae. In Jafri SMH, El-Gadi A (eds) Flora of Libya n. 34. Department of Botany, Faculty of Science, Al Faateh University, Tripoli, pp. 1–27Google Scholar
  16. Jahandiez E, Maire R (1932) Catalogue des plantes du Maroc 2. Imprimerie Minerva, AlgerGoogle Scholar
  17. Kelchner SA (2000) The evolution of non-coding chloroplast DNA and its application in plant systematics. Ann Missouri Bot Gard 87:482–498CrossRefGoogle Scholar
  18. Lange J (1880) Resedaceae. In Willkom M, Lange J (eds) Prodromus Florae Hispanicae 3. E. Schweizerbart, Stuttgart, pp 889–900Google Scholar
  19. Losa España T, Rivas Goday S (1974) Estudio florístico y geobotánico de la provincia de Almería. Arch Inst Aclim 13:197–202Google Scholar
  20. Maire R (1976). Flore de l’Afrique du Nord 14. Éditions Lechevalier, ParisGoogle Scholar
  21. Martín-Bravo S (2009) Resedaceae. In Blanca G, Cabezudo B, Cueto M, Fernández-López C, Morales-Torres C (eds) Flora Vascular de Andalucía Oriental 3. Consejería de Medio Ambiente, Junta de Andalucía, Sevilla, pp 162–168Google Scholar
  22. Martín-Bravo S, Meimberg H, Luceño M, Märkl W, Valcárcel V, Bräuchler C, Vargas P, Heubl G (2007) Molecular systematics and biogeography of Resedaceae based on ITS and trnL-F sequences. Molec Phylogenet Evol 44:1105–1120CrossRefPubMedGoogle Scholar
  23. Mateos MA, Valdés B (2003) Nuevos táxones para el Rif Occidental I. Lagascalia 23:133–156Google Scholar
  24. McNeill J, Barrie FR, Burdet HM, Demoulin V, Hawksworth DL, Marhold K, Nicolson DH, Prado J, Silva PC, Skog JE, Wiersema JH, Turland NJ (2006) International Code of Botanical Nomenclature (Vienna Code). Regnum Veg 146Google Scholar
  25. Médail F, Quézel P (1997) Hot-spots analysis for a conservation of plant biodiversity in the Mediterranean basin. Ann Missouri Bot Gard 84:112–128CrossRefGoogle Scholar
  26. Müller Argoviensis J (1856) Resedaceae aliquot novae v. nondum descriptae. Bot Zeitung (Berlin) 14:33–40Google Scholar
  27. Müller Argoviensis J (1857) Monographie de la famille des Résédacées. Zürcher and Furrer, ZürichGoogle Scholar
  28. Müller Argoviensis J (1868) Resedaceae. In de Candolle AP (ed) Prodromus Systematis Naturalis Regni Vegetabilis 16(2). Victoris Masson et filiis, Paris, pp 548–589Google Scholar
  29. Nylander JAA (2002) MrModeltest version 1.1b. Program distributed by the author. Evolutionary Biology Centre, Uppsala University, UppsalaGoogle Scholar
  30. Peñas de Giles J, Martínez Martínez A, José Sola A, Mota Poveda F (2006) Análisis corológico y novedades florísticas de la Sierra de los Filabres (Andalucía Oriental, España). Lagascalia 26:51–70Google Scholar
  31. Posada D, Buckley TR (2004) Model selection and model averaging in phylogenetics: Advantages of Akaike information criterion and Bayesian approaches over likelihood ratio tests. Syst Biol 53:793–808CrossRefPubMedGoogle Scholar
  32. Quézel P (1978) Analysis of the flora of Mediterranean and Saharan Africa. Ann Missouri Bot Gard 65:479–534CrossRefGoogle Scholar
  33. Quézel P, Santa S (1962) Nouvelle Flore de l´Algérie et des régions désertiques méridionales 1. Editions du centre national de la recherche scientifique, ParisGoogle Scholar
  34. Ronquist F, Huelsenbeck JP (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574CrossRefPubMedGoogle Scholar
  35. Sagredo R (1987) Flora de Almería: plantas vasculares de la provincia. Diputación Provincial, AlmeríaGoogle Scholar
  36. Sennen F (1936) Diagnosis des nouveautés parues dans les exsiccata Plantes d´Espagne et du Maroc de 1928 à 1935. Imp Anglada, VicGoogle Scholar
  37. Swofford DL (2002) PAUP*: Phylogenetic Analysis Using Parsimony (* and other methods). Version 4.0b10. Sinauer Associates, Sunderland, MassachusettsGoogle Scholar
  38. Valdés B (1991) Andalucía and the Rif. Floristic links and common flora. Bot Chron (Patras) 10:117–124Google Scholar
  39. Valdés B (2002) Resedaceae. In Valdés B, Rejdali M, Achhal A, Jury SL, Montserrat JM (eds) Checklist of vascular plants of N Morocco with identification keys 1. Consejo Superior de Investigaciones Científicas, Madrid, pp 265–269Google Scholar
  40. Valdés Bermejo E (1993) Reseda L. In Castroviejo S. et al (eds) Flora Iberica 4. Consejo Superior de Investigaciones Científicas, Madrid, pp 440–475Google Scholar
  41. Yeo PF (1964) Reseda L. In Tutin TG et al (eds) Flora Europaea 1. Ed. 1. Cambridge University Press, Cambridge, pp 346–349Google Scholar
  42. Yeo PF (1996) Reseda L. In Tutin TG et al. (eds) Flora Europaea 1, Ed 2. Cambridge University Press, Cambridge, pp 417–420Google Scholar

Copyright information

© Institute of Botany, Academy of Sciences of the Czech Republic 2009

Authors and Affiliations

  • Santiago Martín-Bravo
    • 1
  • Pedro Jiménez-Mejías
    • 1
  1. 1.Department of Molecular Biology and Biochemical EngineeringPablo de Olavide UniversitySevillaSpain

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