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Folia Geobotanica

, Volume 44, Issue 2, pp 145–158 | Cite as

Regional Speciation or Taxonomic Inflation? The Status of Several Narrowly Distributed and Endangered Species of Narcissus Using ISSR and Nuclear Ribosomal ITS Markers

  • Juan F. Jiménez
  • Pedro Sánchez-Gómez
  • Juan Guerra
  • Arántzazu Molins
  • Josep A. Rosselló
Article

Abstract

The mountains of the southeast Iberian Peninsula harbor several narrowly endemic species belonging to Narcissus section Pseudonarcissi (N. alcaracensis, N. bugei, N. enemeritoi, N. longispathus, N. nevadensis, N. segurensis, and N. yepesii) that are protected by regional Spanish laws. Most of these trumpet daffodils show a very similar overall morphology, and the correct identification of species is difficult unless the geographical origins of the accessions are known. ISSR (Inter-Simple Sequence Repeat) and nuclear ribosomal ITS sequences were used to assess patterns of genetic discontinuities present among the members of this section from southeast Spain. Species-specific ISSR bands were found in N. bugei, N. longispathus and N. nevadensis, but were absent in the other species. The multivariate ordination of the 288 ISSR-genotyped accessions showed that only N. longispathus and N. bugei were clearly differentiated, while the other species formed an unresolved group. ITS sequences of N. bugei were highly divergent from those retrieved from the remaining species, which were placed in a single largely unresolved clade in phylogenetic analysis. In conclusion, ISSR and ITS markers support the taxonomic distinction of N. bugei and N. longispathus. However, N. alcaracensis, N. segurensis, N. yepesii, N. enemeritoi, and N. nevadensis could be merged within a single species (N. nevadensis for nomenclatural priority).

Keywords

Evolution Mediterranean flora Molecular markers Taxonomic inflation Trumpet daffodils 

Notes

Acknowledgements

We are grateful to Dr. C. Herrera and Dr. M. Medrano (Estación Biológica de Doñana, CSIC) for providing samples of N. bugei and N. longispathus. Accessions from Albacete were collected thanks to the help provided by the staff of the Consejería de Medio Ambiente y Desarrollo Rural de Castilla-La Mancha. This work has been partly supported by grants from the Séneca Foundation (PB656/FS02) and the Spanish Ministry of Science and Education (CGL2007-60550/BOS). We thank G. Blanca and J. Darnaude for providing pictures of Narcissus species.

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Copyright information

© Institute of Botany, Academy of Sciences of the Czech Republic 2009

Authors and Affiliations

  • Juan F. Jiménez
    • 1
  • Pedro Sánchez-Gómez
    • 2
  • Juan Guerra
    • 2
  • Arántzazu Molins
    • 1
  • Josep A. Rosselló
    • 1
    • 3
  1. 1.Jardí BotànicUniversidad de ValenciaValenciaSpain
  2. 2.Departamento de Biología Vegetal (Botánica)Universidad de MurciaMurciaSpain
  3. 3.BlanesSpain

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