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Bci528I, a new isoschizomer of EcoRI isolated from Bacillus circulans 528

  • Sung-Ryong RaEmail author
  • Myong-Suk Kim
  • Chon-IL Paek
  • Yong-Chol Pak
  • Song-Hui Pak
  • Hyong-Bom Pak
  • Kum-Chol Ri
Original Article
  • 14 Downloads

Abstract

Bacillus circulans 528 produces a restriction endonuclease, Bci528I which is an isoschizomer of EcoRI. We purified the enzyme, using Sephadex G-150, Phospho-cellulose, DEAE-cellulose, Hepharin-Sepharose CL-6B chromatography. The specific activity of Bci528I was 29,400 U/mg·protein. Bci528I recognizes 5′-GAATTC-3′ in dsDNA and cleaves between G and A of the recognition sequence, producing a symmetric four base 5′overhang.

Notes

Acknowledgements

We thank Dr. Chol-Min Pak of the research institute of biotechnology, academy of science, the DPR of Korea for determination of recognition sequence and cleavage site.

Authors’ contribution

Sung-Ryong Ra conceived and designed the experiments. Myong-Suk Kim and Chon-Il Paek conducted experiments with support from Hyong-Bom Pak, Kum-Chol Ri, and Yong-Chol Pak. Song Hui Pak analyzed the data. Chon-Il Paek wrote the manuscript. All authors read and approved the final manuscript.

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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i. 2019

Authors and Affiliations

  • Sung-Ryong Ra
    • 1
    Email author
  • Myong-Suk Kim
    • 1
  • Chon-IL Paek
    • 1
  • Yong-Chol Pak
    • 1
  • Song-Hui Pak
    • 1
  • Hyong-Bom Pak
    • 1
  • Kum-Chol Ri
    • 2
  1. 1.Research Institute of Biotechnology, Faculty of Life ScienceKim Il Sung UniversityPyongyangDemocratic People’s Republic of Korea
  2. 2.Korean Center for Culture CollectionPyongyangDemocratic People’s Republic of Korea

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