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Folia Microbiologica

, Volume 64, Issue 6, pp 789–796 | Cite as

Molecular detection and identification of Wolbachia endosymbiont in fleas (Insecta: Siphonaptera)

  • Zuhal OnderEmail author
  • Arif Ciloglu
  • Onder Duzlu
  • Alparslan Yildirim
  • Mubeccel Okur
  • Gamze Yetismis
  • Abdullah Inci
Original Article

Abstract

The aim of this study was to determine the presence and prevalence of Wolbachia bacteria in natural population of fleas (Insecta: Siphonaptera) in Turkey, and to exhibit the molecular characterization and the phylogenetic reconstruction at the positive isolates with other species in GenBank, based on 16S rDNA sequences. One hundred twenty-four flea samples belonging to the species Ctenocephalides canis, C. felis, and Pulex irritans were collected from animal shelters in Kayseri between January and August 2017. All flea species were individually screened for the presence of Wolbachia spp. by polymerase chain reaction (PCR) targeting the 16S ribosomal RNA gene. According to PCR analyses, Wolbachia spp. were found prevalent in C. canis and P. irritans fleas, while it was not detected in the C. felis species. Totally, 20 isolates were purified from agarose gel and sequenced with the same primers for molecular characterization and phylogenetic analyses. The sequence analyses revealed 17 polymorphic sites and 2 genetically different Wolbachia isolates, representing two different haplotypes in two flea species. The distribution patterns, molecular characterization, and phylogenetic status of Wolbachia spp. of fleas in Turkey are presented for the first time with this study. Understanding of the role of Wolbachia in vector biology may provide information for developing Wolbachia-based biological control tools.

Notes

Compliance with ethical standards

Conflict of interest

On behalf of all authors, the corresponding author states that there is no conflict of interest.

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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i. 2019

Authors and Affiliations

  • Zuhal Onder
    • 1
    Email author
  • Arif Ciloglu
    • 1
  • Onder Duzlu
    • 1
  • Alparslan Yildirim
    • 1
  • Mubeccel Okur
    • 1
  • Gamze Yetismis
    • 1
  • Abdullah Inci
    • 1
  1. 1.Faculty of Veterinary Medicine, Parasitology DepartmentErciyes UniversityKayseriTurkey

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