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Folia Microbiologica

, Volume 64, Issue 2, pp 197–205 | Cite as

The effect of paclobutrazol on soil bacterial composition across three consecutive flowering stages of mung bean

  • Jimmy Kuo
  • Yen-Wen Wang
  • Michael Chen
  • Georgia Fuh
  • Chorng-Horng LinEmail author
Original Article
  • 52 Downloads

Abstract

Paclobutrazol, (2RS, 3RS)-1-(4-chlorophenyl)-4, 4-dimethyl-2-(1H-1,2,4-triazol-1-yl) pentan-3-ol, is a plant growth retardant that mainly inhibits gibberellins (GAs) biosynthesis. In agricultural practice, paclobutrazol is applied to arrest vegetative growth so as to increase the reproductive growth of many orchard fruit, as well as grain crops. However, due to its over-application and chemical stability, paclobutrazol accumulates in soil and inhibits the growth of subsequent crops, especially those grown for vegetative purposes. The present study focused mainly on the changes in the soil bacterial community following application of paclobutrazol. Mung bean (Vigna radiata) plants were treated with paclobutrazol and cultivated for three consecutive seasons. Soil samples were collected and analyzed by denaturing gradient gel electrophoresis (DGGE) using 16S rDNA gene fragments and clone library analyses. The results obtained through clustering and clonal sequencing analysis showed that the bacterial community was affected by paclobutrazol, and in addition, was more diverse in the third stage of mung bean plant cultivation. The results of the study showed that paclobutrazol affected bacterial composition, and the population of bacteria varied greatly across time.

Notes

Funding information

Financial support was provided partly by grant ORD-9331 from DaYeh University to C.-H. L.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i. 2018

Authors and Affiliations

  1. 1.Department of Planning and ResearchNational Museum of Marine Biology and AquariumPingtungTaiwan
  2. 2.Institute of Marine BiotechnologyNational Dong Hwa UniversityPingtungTaiwan
  3. 3.Institute of Molecular BiologyDaYeh UniversityChanghuaTaiwan
  4. 4.College of the AtlanticBar HarborUSA
  5. 5.Department of BioresourcesDaYeh UniversityChanghuaTaiwan

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