Antagonistic activity of Diplodia pinea against phytopathogenic fungi
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The ability of Diplodia pinea to inhibit Armillaria sp., Bjerkandera adusta, Botrytis cinerea, and Rhizoctonia sp. mycelium growth was analyzed using the double-culture method. Wild-type fungal strains were incubated in a biochemical oxygen demand incubator using potato agar dextrose medium at 24 ± 2 °C for 35 days in darkness. D. pinea significantly inhibited the growth of all fungi species tested (30.75 to 98.37% inhibition) and showed moderate antagonistic activity (antagonistic index, 14.5). Chemical analysis of D. pinea culture broth extracts revealed steroids, triterpenes, and phenolic compounds. Alkaloids were qualitatively detected in the mycelium crude extract. The presence of these compounds may be related to the antagonistic activity observed. The inhibition ability of D. pinea is due to competition with the tested fungi for substrate and space.
The authors are grateful to the Federal University of Paraná and Embrapa Forest for structural support.
This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brasil (CAPES), Finance Code 001.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Badalyan SM, Innocenti G, Garibyan NG (2002) Antagonistic activity of xylotrophic mushrooms against pathogenic fungi of cereals in dual culture. Phytopathol Mediterr 41:220–225. https://doi.org/10.14601/Phytopathol_Mediterr-1668 Google Scholar
- Barnett HL, Hunter BB (1972) Illustrated genera of imperfect fungi. 3.ed. New York: MacMillanGoogle Scholar
- Diekmann MJR, Sutherland DC, Nowell FJ, Morales G (2002) FAO/IPGRI Technical guidelines for the safe movement of germplasm. No. 21. Pinus spp. Food and Agriculture Organization of the United Nations, Rome/International Plant Genetic Resources Institute, RomeGoogle Scholar
- Grigoletti Júnior A, dos Santos ÁF, Auer CG (2005) Perspectivas do uso do controle biológico contra doenças florestais. FLORESTA 30:155–165. doi: https://doi.org/10.5380/rf.v30i12.2362
- Jiang Z, Kempinski C, Chappell J (2017) Extraction and analysis of terpenes/Terpenoids. HHS public Access 345–358. doi: https://doi.org/10.1002/cppb.20024.Extraction
- Liu B, Ichinose T, He M, Kobayashi F, Maki T, Yoshida S, Yoshida Y, Arashidani K, Takano H, Nishikawa M, Sun G, Shibamoto T (2014) Lung inflammation by fungus, Bjerkandera adusta isolated from Asian sand dust (ASD) aerosol and enhancement of ovalbumin-induced lung eosinophilia by ASD and the fungus in mice. Allergy, Asthma Clin Immunol 10:10. https://doi.org/10.1186/1710-1492-10-10 CrossRefGoogle Scholar
- Oliveira CF de, Oliveira VB, Oliveira FF, et al (2014) Quality control parameters of Psychotria fractistipula L.B. Sm., Klein & Delprete (RUBIACEAE): loss on drying, total ash and phytochemical screeningGoogle Scholar
- Ownley BH, Windham MT (2007) Biological control of plant pathogens. In: Trigiano RN, Windham MT, Windham AS (eds) Plant pathology concepts and laboratory exercises, 2nd edn. CRC Press, New YorkGoogle Scholar
- Rayner ADM, Webber JF (1984) Interspecific mycelial interactions: an overview. In: Jennings DH, Rayner ADM (eds) Ecol physiology fungal mycelium. Cambridge Univ Press, Cambridge, pp 383–418Google Scholar
- Regliński T, Rodenburg N, Taylor J, et al (2012) Trichoderma atroviride promotes growth and enhances systemic resistance to Diplodia pinea in radiata pine (Pinus radiata) seedlingsGoogle Scholar
- Riffault L, Destandau E, Pasquier L, et al (2014) Phytochemical analysis of Rosa hybrida cv. “Jardin de Granville” by HPTLC, HPLC-DAD and HPLC-ESI-HRMS: polyphenolic fingerprints of six plant organsGoogle Scholar
- Schoeman M, Webber J, Dickinson DJ (1999) The development of ideas in biological control applied to forest productsGoogle Scholar
- Srivastava BP, Singh KP, Singh U, Pandey VB (1994) Effect of some naturally occurring alkaloids on conidial germination of Botrytis cinerea. Bioved 5:69–72Google Scholar
- Sutton BC (1980) The Coelomycetes. In: Kew. Commonwealth Mycological Institute, SurreyGoogle Scholar
- Valente LMM, Alves FF, Bezerra GM, Almeida MBS, Rosario SL, Mazzei JL, d’Avila LA, Siani AC (2006) Desenvolvimento e aplicação de metodologia por cromatografia em camada delgada para determinação do perfil de alcalóides oxindólicos pentacíclicos nas espécies sul-americanas do gênero Uncaria. Rev Bras Farmacogn 16:216–223CrossRefGoogle Scholar
- Vicente MF, Basilio A, Cabello A, Peláez F (2003) Microbial natural products as a source of antifungals. Clin Microbiol Infect 9(1):15–32. https://doi.org/10.1046/j.1469-0691.2003.00489.x