Folia Microbiologica

, Volume 63, Issue 1, pp 17–22 | Cite as

Trueperella pyogenes isolated from a brain abscess of an adult roebuck (Capreolus capreolus)

  • Jörn-Peter Wickhorst
  • Abdulwahed Ahmed Hassan
  • Omar Hashim Sheet
  • Tobias Eisenberg
  • Osama Sammra
  • Mazen Alssahen
  • Christoph Lämmler
  • Ellen Prenger-Berninghoff
  • Michael Zschöck
  • Markus Timke
  • Amir Abdulmawjood
Original Article
First Online:
Received:
Accepted:

Abstract

The present study was designed to characterize phenotypically and genotypically a Trueperella pyogenes strain isolated from a brain abscess of an adult roebuck (Capreolus capreolus). The species identity could be confirmed by phenotypical investigations, by MALDI-TOF MS analysis, and by sequencing the 16S ribosomal RNA (rRNA) gene, the 16S–23S rRNA intergenic spacer region (ISR); by sequencing the target genes rpoB, gap, and tuf; and by detection of T. pyogenes chaperonin-encoding gene cpn60 with a previously developed loop-mediated isothermal amplification (LAMP) assay. The T. pyogenes strain could additionally be characterized by PCR-mediated amplification of several known and putative virulence factor-encoding genes which revealed the presence of the genes plo encoding pyolysin and nanH and nanP encoding neuraminidases; the genes fimA, fimC, and fimE encoding the fimbrial subunits FimA, FimC, and FimE; and the gene cbpA encoding collagen-binding protein CbpA. The present data give a detailed characterization of a T. pyogenes strain isolated from a brain abscess of a roebuck. However, the route of infection of the roebuck remains unclear.

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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i. 2017

Authors and Affiliations

  • Jörn-Peter Wickhorst
    • 1
  • Abdulwahed Ahmed Hassan
    • 2
  • Omar Hashim Sheet
    • 2
  • Tobias Eisenberg
    • 3
  • Osama Sammra
    • 1
  • Mazen Alssahen
    • 1
  • Christoph Lämmler
    • 1
  • Ellen Prenger-Berninghoff
    • 4
  • Michael Zschöck
    • 3
  • Markus Timke
    • 5
  • Amir Abdulmawjood
    • 6
  1. 1.Institut für Pharmakologie und ToxikologieJustus-Liebig-Universität GießenGiessenGermany
  2. 2.Department of Veterinary Public Health, College of Veterinary MedicineUniversity of MosulMosulIraq
  3. 3.Landesbetrieb Hessisches LandeslaborGiessenGermany
  4. 4.Institut für Hygiene und Infektionskrankheiten der TiereJustus-Liebig-Universität GießenGiessenGermany
  5. 5.Entwicklung BioanalyseBruker Daltonik GmbHBremenGermany
  6. 6.Institute of Food Quality and Food Safety, Research Center for Emerging Infections and Zoonoses (RIZ)University of Veterinary Medicine Hannover, FoundationHannoverGermany

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