Agar-degrading bacteria isolated from Antarctic macroalgae
- 368 Downloads
This study describes the taxonomic diversity of pigmented, agar-degrading bacteria isolated from the surface of macroalgae collected in King George Island, Antarctica. A total of 30 pigmented, agarolytic bacteria were isolated from the surface of the Antarctic macroalgae Adenocystis utricularis, Monostroma hariotii, Iridaea cordata, and Pantoneura plocamioides. Based on the 16S rRNA data, the agarolytic isolates were affiliated to the genera Algibacter, Arthrobacter, Brachybacterium, Cellulophaga, Citricoccus, Labedella, Microbacterium, Micrococcus, Salinibacterium, Sanguibacter, and Zobellia. Isolates phylogenetically related to Cellulophaga algicola showed the highest agarase activity in culture supernatants when tested at 4 and 37 °C. This is the first investigation of pigmented agar-degrading bacteria, members of microbial communities associated with Antarctic macroalgae, and the results suggest that they represent a potential source of cold-adapted agarases of possible biotechnological interest.
KeywordsMarine Agar Agarase Activity Colony Morphotypes Pigment Bacterium Antarctic Macroalgae
We thank the INACH staff at Station Prof. Julio Escudero for logistic support. Special thanks to Dr. Iván Gómez and his group (Project Anillo ART1101) for its valuable support during the field work. This study was supported by Grant RT_06-13 from the Instituto Antártico Chileno (INACH).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Ferrés I, Amarelle V, Noya F, Fabiano E (2015) Identification of Antarctic culturable bacteria able to produce diverse enzymes of potential biotechnological interest. Adv Polar Sci 26:71–79Google Scholar
- Lavin P, Gallardo-Cerda J, Torres-Diaz C, Asencio G, Gonzalez M (2013) Antarctic strain of Bacillus sp. with extracellular agarolitic and alginate-lyase activities. Gayana 77:75–82Google Scholar
- McBride MJ (2014) The family Flavobacteriaceae. In: Rosenberg E et al (eds) The prokaryotes. Springer-Verlag, Berlin, pp 643–676Google Scholar
- Michel G, Czjzek M, Trincone A (2013) Polysaccharide-degrading enzymes from marine bacteria. In: Trincone A (ed) Marine Enzymes for Biocatalysis: Sources, Biocatalytic Characteristics and Bioprocesses of Marine Enzymes. Woodhead Publishing, pp 429–464Google Scholar
- Nedashkovskaya OI, Suzuki M, Vancanneyt M, Cleenwerck I, Lysenko AM, Mikhailov VV, Swings J (2004b) Zobellia amurskyensis sp. nov., Zobellia laminariae sp. nov. and Zobellia russellii sp. nov., novel marine bacteria of the family Flavobacteriaceae. Int J Syst Evol Micr 54:1643–1648CrossRefGoogle Scholar
- Ramírez ME (2010) Flora marina bentónica de la región austral de Sudamérica y la Antártica. An Inst Patagonia 38:57–71Google Scholar
- Reddy PVV, Rao SSSN, Pratibha MS, Sailaja B, Kavya B, Manorama RR, Singh SM, Srinivas TNR, Shivaji S (2009) Bacterial diversity and bioprospecting for cold-active enzymes from culturable bacteria associated with sediment from a melt water stream of Midtre lo enbreen glacier, an Arctic glacier. Res Microbiol 160:538–546CrossRefGoogle Scholar
- Srinivas TNR, Rao SSSN, Reddy PVV, Pratibha MS, Sailaja B, Kavya B, Kishore KH, Begum Z, Singh SM, Shivaji S (2009) Bacterial diversity and bioprospecting for cold-active lipases, amylases and proteases, from culturable bacteria of Kongsfjorden and Ny-Ålesund, Svalbard, Arctic. Curr Microbiol 59:537–547CrossRefPubMedGoogle Scholar
- Tropeano M, Vazquez S, Coria S, Turjanski A, Cicero D, Bercovich A, Mac Cormack W (2013) Extracellular hydrolytic enzyme production by proteolytic bacteria from the Antarctic. Pol Polar Res 34:253–267Google Scholar