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Folia Microbiologica

, Volume 62, Issue 4, pp 325–334 | Cite as

Isolation of Pseudomonas fluorescens species highly resistant to pentachlorobenzene

  • Itxaso Montánchez
  • Anna Chao Kaberdina
  • Elena Sevillano
  • Lucía Gallego
  • Susana Rodríguez-Couto
  • Vladimir R. KaberdinEmail author
Article

Abstract

Polychlorinated aromatic compounds, including pentachlorobenzenes and hexachlorobenzenes, are recalcitrant industrial pollutants that cause adverse effects on living cells. In this paper, the isolation of Pseudomonas fluorescens species with high resistance to pentachlorobenzene (PeCB) is reported. It was found that, in contrast to its slightly negative effect on P. fluorescens growth, PeCB readily inhibited the cell growth of Serratia spp. and Escherichia coli strains, thus indicating that inhibition of bacterial growth by PeCB is species-dependent. Analysis of a P. fluorescens isolate revealed that the exposure to PeCB induced production of reactive oxygen species and led to an increase in the level of alkyl hydroperoxide reductase C (AhpC), an important enzyme enhancing the cell tolerance to organic hydroperoxides usually accumulated under oxidative stress. The putative mechanism conferring PeCB resistance to P. fluorescens and the potential use of P. fluorescens in bioremediation are discussed.

Keywords

Cell adaptation Polychlorinated benzenes Toxic compounds Oxidative stress Alkyl hydroperoxide reductase C Reactive oxygen species (ROS) 

Notes

Acknowledgements

We are grateful to the staff of the Genomics and Proteomics Units at the Advanced Core Research Facilities (SGIker) of the University of the Basque Country (UPV/EHU) for the technical support and assistance provided. The work was supported by the Basque Government (grant PRE-2013-1-901 and SAIOTEK grant S-PE12UN84) and by IKERBASQUE (Basque Foundation for Science).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

12223_2017_501_MOESM1_ESM.pdf (85 kb)
ESM 1 (PDF 85 kb)
12223_2017_501_MOESM2_ESM.pdf (25 kb)
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12223_2017_501_MOESM3_ESM.docx (27 kb)
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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i. 2017

Authors and Affiliations

  • Itxaso Montánchez
    • 1
  • Anna Chao Kaberdina
    • 2
  • Elena Sevillano
    • 1
  • Lucía Gallego
    • 1
  • Susana Rodríguez-Couto
    • 3
    • 4
  • Vladimir R. Kaberdin
    • 1
    • 4
    • 5
    Email author
  1. 1.Department of Immunology, Microbiology and ParasitologyUniversity of the Basque Country UPV/EHULeioaSpain
  2. 2.Department of Biochemistry and Molecular BiologyUniversity of the Basque Country (UPV/EHU)BilbaoSpain
  3. 3.Unit of Environmental EngineeringCeit-IK4San SebastianSpain
  4. 4.IKERBASQUE, Basque Foundation for ScienceBilbaoSpain
  5. 5.Research Centre for Experimental Marine Biology and Biotechnology (PIE-UPV/EHU)PlentziaSpain

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