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Folia Microbiologica

, Volume 61, Issue 6, pp 465–471 | Cite as

Evaluation of capacity to detect ability to form biofilm in Candida parapsilosis sensu stricto strains by MALDI-TOF MS

  • Katarína Mlynáriková
  • Ondrej Šedo
  • Filip RůžičkaEmail author
  • Zbyněk Zdráhal
  • Veronika Holá
  • Martina Mahelová
Article

Abstract

Matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is, currently, used as a rapid and reliable tool in microbial diagnostics. The discriminatory power of the method extends its applicability also beyond species level. This study examined the possibility to use MALDI-TOF MS to differentiate between Candida parapsilosis sensu stricto biofilm-positive (n = 12) and biofilm-negative (n = 9) strains. The results indicated a grouping trend within MALDI-TOF mass spectra belonging to each of the tested groups. However, these trends were eclipsed by mass spectral variations resulting from limited repeatability of the method, making its application for the selected purpose impossible. Improvement in the discriminatory power of the method was not obtained neither by using different matrices (α-cyano-4-hydroxycinnamic acid, ferulic acid, 5-chloro-2-mercaptobenzothionazole) for MALDI-TOF MS analysis nor by testing different culture conditions (cultivation length, culture media).

Keywords

Planktonic Culture Repeated Cultivation Obtain Mass Spectrum MALDI Matrice Yeast Nitrogen Base Medium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work was supported by Proteomics Core Facility of CEITEC—Central European Institute of Technology (ID number CZ.1.05/1.1.00/02.0068) and financed by the European Regional Development Fund, by the Ministry of Education, Youth and Sports of the Czech Republic (the Specific University Research Grant MUNI/A/0964/2015), and by the Ministry of Health of the Czech Republic (No. 16-29916A).

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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i. 2016

Authors and Affiliations

  • Katarína Mlynáriková
    • 1
  • Ondrej Šedo
    • 2
    • 3
  • Filip Růžička
    • 1
    • 4
    Email author
  • Zbyněk Zdráhal
    • 2
    • 3
  • Veronika Holá
    • 1
    • 4
  • Martina Mahelová
    • 1
  1. 1.Department of Microbiology, Faculty of MedicineMasaryk UniversityBrnoCzech Republic
  2. 2.Research Group Proteomics, Central European Institute of TechnologyMasaryk UniversityBrnoCzech Republic
  3. 3.National Centre of Biomolecular Research, Faculty of ScienceMasaryk UniversityBrnoCzech Republic
  4. 4.Department of MicrobiologySt. Anne’s Faculty HospitalBrnoCzech Republic

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