Evaluation of capacity to detect ability to form biofilm in Candida parapsilosis sensu stricto strains by MALDI-TOF MS
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Matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is, currently, used as a rapid and reliable tool in microbial diagnostics. The discriminatory power of the method extends its applicability also beyond species level. This study examined the possibility to use MALDI-TOF MS to differentiate between Candida parapsilosis sensu stricto biofilm-positive (n = 12) and biofilm-negative (n = 9) strains. The results indicated a grouping trend within MALDI-TOF mass spectra belonging to each of the tested groups. However, these trends were eclipsed by mass spectral variations resulting from limited repeatability of the method, making its application for the selected purpose impossible. Improvement in the discriminatory power of the method was not obtained neither by using different matrices (α-cyano-4-hydroxycinnamic acid, ferulic acid, 5-chloro-2-mercaptobenzothionazole) for MALDI-TOF MS analysis nor by testing different culture conditions (cultivation length, culture media).
KeywordsPlanktonic Culture Repeated Cultivation Obtain Mass Spectrum MALDI Matrice Yeast Nitrogen Base Medium
This work was supported by Proteomics Core Facility of CEITEC—Central European Institute of Technology (ID number CZ.1.05/1.1.00/02.0068) and financed by the European Regional Development Fund, by the Ministry of Education, Youth and Sports of the Czech Republic (the Specific University Research Grant MUNI/A/0964/2015), and by the Ministry of Health of the Czech Republic (No. 16-29916A).
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