Advertisement

Folia Microbiologica

, Volume 61, Issue 5, pp 359–363 | Cite as

Detection of Borrelia burgdorferi sensu stricto and Borrelia garinii DNAs in patient with Hyperkeratosis lenticularis perstans (Flegel disease)

  • Katarina SchwarzovaEmail author
  • Peter Kozub
  • Zoltan Szep
  • Marina Golovchenko
  • Natasha Rudenko
Article

Abstract

Determination of the causative agent of erythema-like skin lesions in case of nonspecific superficial perivascular dermatitis was supported by histological examination and led to the latter diagnosis of Hyperkeratosis lenticularis perstans (Flegel disease) in patient. The presence of antibodies against Borrelia burgdorferi in patient serum was confirmed by a routine ELISA method and verified by Western blot technique. Skin biopsy and blood specimens were analyzed by PCR and multilocus sequence analysis (MLSA). Western blot method revealed IgG antibody response against two specific antigens, 17 and 83 kDa proteins. The recombinant test detected IgG antibody response against p100 and p41 antigens. The sequence analysis of amplicons from the selected genomic loci obtained from skin biopsy and serum samples revealed the presence of two species from B. burgdorferi sensu lato complex as a co-infection in this patient—B. burgdorferi sensu stricto (s.s.) and Borrelia garinii.

Keywords

Skin Biopsy Borrelia Burgdorferi Lyme Borreliosis Calcipotriol Acitretin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Compliance with ethical standard

Conflict of interest

The authors declare that they have no competing interests.

References

  1. Bunikis J, Garpmo U, Tsao J, Berglund J, Fish D, Barbour AG (2004) Sequence typing reveals extensive strain diversity of the Lyme borreliosis agents Borrelia burgdorferi in North America and Borrelia afzelii in Europe. Microbiology 150:1741–1755CrossRefPubMedGoogle Scholar
  2. Clark K, Hendricks A, Burge D (2005) Molecular identification and analysis of Borrelia burgdorferi sensu lato in lizards in the southeastern United States. Appl Environ Microbiol 71:2616–2625CrossRefPubMedPubMedCentralGoogle Scholar
  3. Girard YA, Fedorova N, Lane RS (2011) Genetic diversity of Borrelia burgdorferi and detection of B. bissettii-like DNA in serum of north-coastal California residents. J Clin Microbiol 49:945–954CrossRefPubMedPubMedCentralGoogle Scholar
  4. Krishnan A, Kar S (2012) Hyperkeratosis lenticularis perstans (Flegel’s disease) with unusual clinical presentation. Response to isotretinoin therapy. J Dermatol Case Rep 6(3):93–95CrossRefPubMedPubMedCentralGoogle Scholar
  5. Margos G, Gatewood AG, Aanensen DM, Hanincová K, Terekhova D, Vollmer SA, Cornet M, Piesman J, Donaghy M, Bormane A, Hurn MA, Feil EJ, Fish D, Casjens S, Wormser GP, Schwartz I, Kurtenbach K (2008) MLST of housekeeping genes captures geographic population structure and suggests a European origin of Borrelia burgdorferi. Proc Natl Acad Sci U S A 105:8730–8735CrossRefPubMedPubMedCentralGoogle Scholar
  6. Metze D, Lubke D, Luger T (2000) Hyperkeratosis lenticularis perstans (Flegel’s disease)—a complex disorder of epidermal differentiation with good response to a synthetic vitamin D3 derivate. Hautarzt 51(1):31–5CrossRefPubMedGoogle Scholar
  7. Middelveen MJ, Poruri A, Mayne PJ, Sapi E, Kahn DG, Stricker RB (2013) Association of Borrelia burgdorferi infection with Morgellons disease. J Investig Med 61:225Google Scholar
  8. Middelveen MJ, Bandoski C, Burke J, Sapi E, Filush KR, Wang Y, Franco A, Mayne PJ, Stricker RB (2015) Exploring the association between Morgellons disease and lyme disease identification of Borrelia burgdorferi in Morgellons disease patients. BMC Dermatol 15(1). doi: 10.1186/s12895-015-0023-0
  9. Rudenko N, Golovchenko M, Němec J, Volkaer J, Mallátová N, Grubhoffer L (2005) Improved method of detection and molecular typing of Borrelia burgdorferi sensu lato in clinical sample by PCR without DNA purification. Folia Microbiol 50:31–39CrossRefGoogle Scholar
  10. Rudenko N, Golovchenko M, Mokracek A, Piskunova N, Ruzek D, Mallatova N, Grubhoffer L (2008) Detection of Borrelia bissettii in cardiac valve tissue of a patient with endocarditis and aortic valve stenosis in the Czech Republic. J Clin Microbiol 46:3540–3543CrossRefPubMedPubMedCentralGoogle Scholar
  11. Rudenko N, Golovchenko M, Ruzek D, Piskunova N, Mallatova N, Grubhoffer L (2009) Molecular detection of Borrelia bissettii DNA in serum samples from patients in the Czech Republic with suspected borreliosis. FEMS Microbiol Lett 292:274–281CrossRefPubMedGoogle Scholar
  12. Rudenko N, Golovchenko M, Grubhoffer L, Oliver JH Jr (2011) Updates on Borrelia burgdorferi sensu lato complex with respect to public health. Ticks Tick Borne Dis 2:123–128CrossRefPubMedPubMedCentralGoogle Scholar
  13. Schwarzova K, Kostanova Z, Holeckova K, Spitalska E, Boldis V (2009) Direct detection of Borrelia burgdorferi spirochetes in patients with early disseminated Lyme borreliosis. Cent Eur J Public Health 17(4):179–182PubMedGoogle Scholar

Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i. 2016

Authors and Affiliations

  • Katarina Schwarzova
    • 1
    Email author
  • Peter Kozub
    • 2
  • Zoltan Szep
    • 3
  • Marina Golovchenko
    • 4
  • Natasha Rudenko
    • 4
  1. 1.Institute of Microbiology, Faculty of MedicineComenius University in Bratislava and University HospitalBratislavaSlovakia
  2. 2.Department of DermatologyFaculty Hospital with Policlinic in Nove ZamkyNove ZamkySlovakia
  3. 3.Cytopatos s.r.o., Dermatopathology UnitBratislavaSlovakia
  4. 4.Biology Center, Institute of Parasitology CASČeské BudějoviceCzech Republic

Personalised recommendations