Folia Microbiologica

, Volume 61, Issue 5, pp 359–363 | Cite as

Detection of Borrelia burgdorferi sensu stricto and Borrelia garinii DNAs in patient with Hyperkeratosis lenticularis perstans (Flegel disease)

  • Katarina SchwarzovaEmail author
  • Peter Kozub
  • Zoltan Szep
  • Marina Golovchenko
  • Natasha Rudenko


Determination of the causative agent of erythema-like skin lesions in case of nonspecific superficial perivascular dermatitis was supported by histological examination and led to the latter diagnosis of Hyperkeratosis lenticularis perstans (Flegel disease) in patient. The presence of antibodies against Borrelia burgdorferi in patient serum was confirmed by a routine ELISA method and verified by Western blot technique. Skin biopsy and blood specimens were analyzed by PCR and multilocus sequence analysis (MLSA). Western blot method revealed IgG antibody response against two specific antigens, 17 and 83 kDa proteins. The recombinant test detected IgG antibody response against p100 and p41 antigens. The sequence analysis of amplicons from the selected genomic loci obtained from skin biopsy and serum samples revealed the presence of two species from B. burgdorferi sensu lato complex as a co-infection in this patient—B. burgdorferi sensu stricto (s.s.) and Borrelia garinii.


Skin Biopsy Borrelia Burgdorferi Lyme Borreliosis Calcipotriol Acitretin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Compliance with ethical standard

Conflict of interest

The authors declare that they have no competing interests.


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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i. 2016

Authors and Affiliations

  • Katarina Schwarzova
    • 1
    Email author
  • Peter Kozub
    • 2
  • Zoltan Szep
    • 3
  • Marina Golovchenko
    • 4
  • Natasha Rudenko
    • 4
  1. 1.Institute of Microbiology, Faculty of MedicineComenius University in Bratislava and University HospitalBratislavaSlovakia
  2. 2.Department of DermatologyFaculty Hospital with Policlinic in Nove ZamkyNove ZamkySlovakia
  3. 3.Cytopatos s.r.o., Dermatopathology UnitBratislavaSlovakia
  4. 4.Biology Center, Institute of Parasitology CASČeské BudějoviceCzech Republic

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