Detection of Borrelia burgdorferi sensu stricto and Borrelia garinii DNAs in patient with Hyperkeratosis lenticularis perstans (Flegel disease)
Determination of the causative agent of erythema-like skin lesions in case of nonspecific superficial perivascular dermatitis was supported by histological examination and led to the latter diagnosis of Hyperkeratosis lenticularis perstans (Flegel disease) in patient. The presence of antibodies against Borrelia burgdorferi in patient serum was confirmed by a routine ELISA method and verified by Western blot technique. Skin biopsy and blood specimens were analyzed by PCR and multilocus sequence analysis (MLSA). Western blot method revealed IgG antibody response against two specific antigens, 17 and 83 kDa proteins. The recombinant test detected IgG antibody response against p100 and p41 antigens. The sequence analysis of amplicons from the selected genomic loci obtained from skin biopsy and serum samples revealed the presence of two species from B. burgdorferi sensu lato complex as a co-infection in this patient—B. burgdorferi sensu stricto (s.s.) and Borrelia garinii.
KeywordsSkin Biopsy Borrelia Burgdorferi Lyme Borreliosis Calcipotriol Acitretin
Compliance with ethical standard
Conflict of interest
The authors declare that they have no competing interests.
- Margos G, Gatewood AG, Aanensen DM, Hanincová K, Terekhova D, Vollmer SA, Cornet M, Piesman J, Donaghy M, Bormane A, Hurn MA, Feil EJ, Fish D, Casjens S, Wormser GP, Schwartz I, Kurtenbach K (2008) MLST of housekeeping genes captures geographic population structure and suggests a European origin of Borrelia burgdorferi. Proc Natl Acad Sci U S A 105:8730–8735CrossRefPubMedPubMedCentralGoogle Scholar
- Middelveen MJ, Poruri A, Mayne PJ, Sapi E, Kahn DG, Stricker RB (2013) Association of Borrelia burgdorferi infection with Morgellons disease. J Investig Med 61:225Google Scholar
- Middelveen MJ, Bandoski C, Burke J, Sapi E, Filush KR, Wang Y, Franco A, Mayne PJ, Stricker RB (2015) Exploring the association between Morgellons disease and lyme disease identification of Borrelia burgdorferi in Morgellons disease patients. BMC Dermatol 15(1). doi: 10.1186/s12895-015-0023-0