Folia Microbiologica

, Volume 61, Issue 2, pp 159–168

The isolation and characterization of actinobacteria from dominant benthic macroinvertebrates endemic to Lake Baikal

  • Denis Axenov-Gribanov
  • Yuriy Rebets
  • Bogdan Tokovenko
  • Irina Voytsekhovskaya
  • Maxim Timofeyev
  • Andriy Luzhetskyy
Article

DOI: 10.1007/s12223-015-0421-z

Cite this article as:
Axenov-Gribanov, D., Rebets, Y., Tokovenko, B. et al. Folia Microbiol (2016) 61: 159. doi:10.1007/s12223-015-0421-z

Abstract

The high demand for new antibacterials fosters the isolation of new biologically active compounds producing actinobacteria. Here, we report the isolation and initial characterization of cultured actinobacteria from dominant benthic organisms’ communities of Lake Baikal. Twenty-five distinct strains were obtained from 5 species of Baikal endemic macroinvertebrates of amphipods, freshwater sponges, turbellaria worms, and insects (caddisfly larvae). The 16S ribosomal RNA (rRNA)-based phylogenic analysis of obtained strains showed their affiliation to Streptomyces, Nocardia, Pseudonocardia, Micromonospora, Aeromicrobium, and Agromyces genera, revealing the diversity of actinobacteria associated with the benthic organisms of Lake Baikal. The biological activity assays showed that 24 out of 25 strains are producing compounds active against at least one of the test cultures used, including Gram-negative bacteria and Candida albicans. Complete dereplication of secondary metabolite profiles of two isolated strains led to identification of only few known compounds, while the majority of detected metabolites are not listed in existing antibiotic databases.

Supplementary material

12223_2015_421_MOESM1_ESM.docx (8.4 mb)
ESM 1(DOCX 8618 kb)

Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i. 2015

Authors and Affiliations

  • Denis Axenov-Gribanov
    • 1
  • Yuriy Rebets
    • 2
  • Bogdan Tokovenko
    • 2
  • Irina Voytsekhovskaya
    • 1
  • Maxim Timofeyev
    • 1
  • Andriy Luzhetskyy
    • 2
  1. 1.Institute of Biology at Irkutsk State UniversityIrkutskRussia
  2. 2.Helmholtz Institute for Pharmaceutical Research SaarlandSaarbruckenGermany

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