The isolation and characterization of actinobacteria from dominant benthic macroinvertebrates endemic to Lake Baikal
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The high demand for new antibacterials fosters the isolation of new biologically active compounds producing actinobacteria. Here, we report the isolation and initial characterization of cultured actinobacteria from dominant benthic organisms’ communities of Lake Baikal. Twenty-five distinct strains were obtained from 5 species of Baikal endemic macroinvertebrates of amphipods, freshwater sponges, turbellaria worms, and insects (caddisfly larvae). The 16S ribosomal RNA (rRNA)-based phylogenic analysis of obtained strains showed their affiliation to Streptomyces, Nocardia, Pseudonocardia, Micromonospora, Aeromicrobium, and Agromyces genera, revealing the diversity of actinobacteria associated with the benthic organisms of Lake Baikal. The biological activity assays showed that 24 out of 25 strains are producing compounds active against at least one of the test cultures used, including Gram-negative bacteria and Candida albicans. Complete dereplication of secondary metabolite profiles of two isolated strains led to identification of only few known compounds, while the majority of detected metabolites are not listed in existing antibiotic databases.
KeywordsStreptomyces Agmatine Sulfamic Acid Freshwater Sponge Biomass Extract
This research was supported by the Ministry of Education and Science of the Russian Federation as a part of Goszadanie projects (No. 6.382.2014/K), Russian Science Foundation (project N 14-14-00400), Russian Foundation for Basic Research (projects N 14-04-00501, 15-04-06685), US Civilian Research & Development Foundation (project N FSCX-15-61168-0), grants of Irkutsk State University for young researchers, and Deutscher Akademischer Austauschdienst.
Conflict of interest
The authors declare that they have no competing interests.
Statement of human rights
All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.
Statement on the welfare of animals
This article does not contain any studies with human participants or vertebrate animals performed by any of the authors.
Mentioned in the article were Baikalian macroinvertebrate species not involved in endangered or protected species. No specific permissions were required for the sampling of invertebrate species.
Informed consent was obtained from all individual participants included in the study.
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