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Folia Microbiologica

, Volume 60, Issue 5, pp 457–464 | Cite as

Precursor directed biosynthesis of odd-numbered fatty acids by different yeasts

  • Tomáš ŘezankaEmail author
  • Irena Kolouchová
  • Karel Sigler
Article

Abstract

Precursor-directed biosynthesis was used for directed preparation of positional isomers of heptadecanoic acid (17:1), which have convenient pharmacological properties. Cultivation of Candida sp., Kluyveromyces polysporus, Rhodotorula glutinis, Saccharomyces cerevisiae, Torulaspora delbrueckii, Trichosporon cutaneum, and Yarrowia lipolytica on 20 g/L glucose, 4 g/L acetic, or 4 g/L propionic acids yielded different proportions of 17:1. Cultivation on carbon sources with even numbers of carbon atoms (glucose and acetic acid) produced preferentially 8Z- and 10Z-heptadecenoic acids in about equal amounts, in agreement with the proposed biosynthesis of fatty acids, whereas cultivation on propionic acid as the only carbon source produced over 90 % of total fatty acids of 9-17:1 out of all possible positional isomers. The structures of positional isomers of 17:1 acid were determined using dimethyl disulfides of fatty acid methyl esters. In cultivation of Candida sp. on propionic acid, the yield of heptadecenoic acid reached 111 mg/L cultivation medium. Principal component analysis was used for identifying the effect of cultivation conditions on the production of the 17:1 acid by individual yeast strains.

Keywords

Powdery Mildew Propionic Acid Positional Isomer Dimethyl Disulfide Picolinyl 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The research was supported by GACR projects P503/11/0215 and P503 14-00227S, and by Institutional Research Concept RVO61388971.

Supplementary material

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12223_2015_388_MOESM2_ESM.doc (43 kb)
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12223_2015_388_MOESM3_ESM.doc (43 kb)
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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i. 2015

Authors and Affiliations

  • Tomáš Řezanka
    • 1
    Email author
  • Irena Kolouchová
    • 2
  • Karel Sigler
    • 1
  1. 1.Institute of MicrobiologyAcademy of Sciences of the Czech RepublicPragueCzech Republic
  2. 2.Department of BiotechnologyUniversity of Chemistry and Technology PraguePragueCzech Republic

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