Advertisement

Folia Microbiologica

, Volume 59, Issue 5, pp 369–374 | Cite as

Phenotypic and genotypic characteristics of Arcanobacterium haemolyticum isolated from clinical samples in a Danish hospital

  • Osama Sammra
  • Alice Friis-Møller
  • Anna Balbutskaya
  • Muaz Hijazin
  • Samy Nagib
  • Jörg Alber
  • Christoph LämmlerEmail author
  • Amir Abdulmawjood
  • Markus Timke
  • Markus Kostrzewa
  • Ellen Prenger-Berninghoff
Article

Abstract

Six Arcanobacterium haemolyticum strains isolated from six patients of two hospitals in Denmark were identified phenotypically, also including matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis, and by genotypic methods. The latter were performed by sequencing 16S rDNA and glyceraldehyde 3-phosphate dehydrogenase encoding gene gap and by amplification of an A. haemolyticum specific region of 16S–23S rDNA intergenic spacer region and 23S rDNA. The six A. haemolyticum strains were further investigated for the presence of seven potential virulence genes encoding arcanolysin, phospholipase D, hemolysin A, CAMP factor family protein, collagen binding protein, neuraminidase A and neuraminidase H which appeared to be present in two (seven virulence genes), two (six virulence genes) and two strains (four virulence genes), respectively. The phenotypic and genotypic properties described in the present study might help to reliably identify and further characterize A. haemolyticum isolated from human patients, a species which seems to be of increasing importance.

Keywords

Streptococcus Agalactiae Potential Virulence Factor Hemolytic Property Collagen Binding Protein Haemolyticum Strain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Balikci A, Topkaya AE, Belaş Z (2011) A frequently overlooked bacteria in clinical microbiology laboratories: Arcanobacterium haemolyticum. Mikrobiyol Bul 45:535–540PubMedGoogle Scholar
  2. Banck G, Nyman M (1986) Tonsillitis and rash associated with Corynebacterium haemolyticum. J Infect Dis 154:1037–1040PubMedCrossRefGoogle Scholar
  3. Brown J, Fleming CS, Troia-Cancio PV (2013) Arcanobacterium haemolyticum osteomyelitis and sepsis: a diagnostic conundrum. Surg Infect (Larchmt) 14:322–324CrossRefGoogle Scholar
  4. Collins MD, Jones D, Schofield GM (1982) Reclassification of ‘Corynebacterium haemolyticum’ (MacLean, Liebow and Rosenberg) in the genus Arcanobacterium gen. nov. as Arcanobacterium haemolyticum nom. rev., comb. nov. J Gen Microbiol 128:1279–1281PubMedGoogle Scholar
  5. García-de-la-Fuente C, Ruiz De Alegría C, Cano ME, Campo AB, Unda F, Agüero J, Martínez-Martínez (2012) Phenotypic and molecular characterization of Arcanobacterium haemolyticum isolated from clinical samples. Diagn Microbiol Infect Dis 72:1–7PubMedCrossRefGoogle Scholar
  6. Hassan AA, Ülbegi-Mohyla H, Kanbar T, Alber J, Lämmler C, Abdulmawjood A, Zschöck M, Weiss R (2009) Phenotypic and genotypic characterization of Arcanobacterium haemolyticum isolates from infections of horses. J Clin Microbiol 47:124–128PubMedCentralPubMedCrossRefGoogle Scholar
  7. Hijazin M, Ülbegi-Mohyla H, Alber J, Lämmler C, Hassan AA, Prenger-Berninghoff E, Weiss R, Zschöck M (2010) Identification of Arcanobacterium haemolyticum isolated from postcastrational complications of a horse. Folia Microbiol (Praha) 55:666–668CrossRefGoogle Scholar
  8. Hijazin M, Hassan AA, Alber J, Lämmler C, Timke M, Kostrzewa M, Prenger-Berninghoff E, Zschöck M (2012a) Evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for species identification of bacteria of genera Arcanobacterium and Trueperella. Vet Microbiol 157:243–245PubMedCrossRefGoogle Scholar
  9. Hijazin M, Prenger-Berninghoff E, Sammra O, Alber J, Lämmler C, Kämpfer P, Glaeser SP, Busse HJ, Hassan AA, Abdulmawjood A, Zschöck M (2012b) Arcanobacterium canis sp. nov., isolated from otitis externa of a dog, and emended description of the genus Arcanobacterium Collins et al. 1983 emend. Yassin et al. 2011. Int J Syst Evol Microbiol 62:2201–2205PubMedCrossRefGoogle Scholar
  10. Hijazin M, Sammra O, Ülbegi H, Nagib S, Alber J, Lämmler C, Kämpfer P, Busse HJ, Glaeser SP, Kassmannhuber J, Prenger-Berninghoff E, Weiss R, Siebert U, Hassan AA, Abdulmawjood A, Zschöck M (2013) Arcanobacterium phocisimile sp. nov., isolated from harbour seals. Int J Syst Evol Microbiol 63:2019–2024PubMedCrossRefGoogle Scholar
  11. Jost BH, Billington SJ (2005) Arcanobacterium pyogenes: molecular pathogenesis of an animal opportunist. Antonie Van Leeuwenhoek 88:87–102PubMedCrossRefGoogle Scholar
  12. Jost BH, Lucas EA, Billington SJ, Ratner AJ, Mcgee DJ (2011) Arcanolysin is a cholesterol-dependent cytolysin of the human pathogen Arcanobacterium haemolyticum. BMC Microbiol 11:239–250PubMedCentralPubMedCrossRefGoogle Scholar
  13. Lämmler C, Hartwigk H (1995) Actinomyces pyogenes und Arcanobacterium haemolyticum. In: Blobel H, Schließer T (eds) Handbuch der bakteriellen Infektionen bei Tieren, Band II/3, 2nd edn. Gustav Fischer Verlag, Jena, pp 196–240Google Scholar
  14. Lucas EA (2009) Identification and characterization of Arcanobacterium haemolyticum virulence factors. Tucson, Microbiology, Pro Quest. University of Arizona, TucsonGoogle Scholar
  15. Lucas EA, Billington SJ, Carlson P, Mcgee DJ, Jost BH (2010) Phospholipase D promotes Arcanobacterium haemolyticum adhesion via lipid raft remodeling and host cell death following bacterial invasion. BMC Microbiol 10:270–281PubMedCentralPubMedCrossRefGoogle Scholar
  16. Maclean PD, Liebow AA, Rosenberg AA (1946) A hemolytic Corynebacterium resembling Corynebacterium ovis and Corynebacterium pyogenes in man. J Infect Dis 79:69–90PubMedCrossRefGoogle Scholar
  17. Miller RA, Brancato F, Holmes KK (1986) Corynebacterium hemolyticum as a cause of pharyngitis and scarlatiniform rash in young adults. Ann Intern Med 105:867–872PubMedCrossRefGoogle Scholar
  18. Murray PR (2010) Matrix-assisted laser desorption ionization time-of-flight mass spectrometry: usefulness for taxonomy and epidemiology. Clin Microbiol Infect 16:1626–1630PubMedCrossRefGoogle Scholar
  19. Sammra O, Balbutskaya A, Nagib S, Alber J, Lämmler C, Abdulmawjood A, Timke M, Kostrzewa M, Prenger-Berninghoff E (2014) Properties of an Arcanobacterium haemolyticum strain isolated from a donkey. Berl Münch Tierärztl Wochenschr 127:10–14Google Scholar
  20. Seng P, Drancourt M, Gouriet F, La Scola B, Fournier PE, Rolain JM, Raoult D (2009) Ongoing revolution in bacteriology: routine identification of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Clin Infect Dis 49:543–551PubMedCrossRefGoogle Scholar
  21. Skov RL, Sanden AK, Danchell VH, Robertsen K, Ejlertsen T (1998) Systemic and deep-seated infections caused by Arcanobacterium haemolyticum. Eur J Clin Microbiol Infect Dis 17:578–582PubMedGoogle Scholar
  22. Tan TY, Ng SY, Thomas H, Chan BK (2006) Arcanobacterium haemolyticum bacteraemia and soft-tissue infections: case report and review of the literature. J Infect 53:69–74CrossRefGoogle Scholar
  23. Therriault BL, Daniels LM, Carter YL, Raasch RH (2008) Severe sepsis caused by Arcanobacterium haemolyticum: a case report and review of the literature. Ann Pharmacother 42:1697–1702PubMedCrossRefGoogle Scholar
  24. Ülbegi-Mohyla H, Hassan AA, Kanbar T, Alber J, Lämmler C, Prenger-Berninghoff E, Weiss R, Siebert U, Zschöck M (2009) Synergistic and antagonistic hemolytic activities of bacteria of genus Arcanobacterium and CAMP-like hemolysis of Arcanobacterium phocae and Arcanobacterium haemolyticum with Psychrobacter phenylpyruvicus. Res Vet Sci 87:186–188PubMedCrossRefGoogle Scholar
  25. Yasawong M, Teshima H, Lapidus A, Nolan M, Lucas S, Glavina Del Rio T, Tice H, Cheng JF, Bruce D, Detter C, Tapia R, Han C, Goodwin L, Pitluck S, Liolios K, Ivanova N, Mavromatis K, Mikhailova N, Pati A, Chen A, Palaniappan K, Land M, Hauser L, Chang YJ, Jeffries CD, Rohde M, Sikorski J, Pukall R, Goker M, Woyke T, Bristow J, Eisen JA, Markowitz V, Hugenholtz P, Kyrpides NC, Klenk HP (2010) Complete genome sequence of Arcanobacterium haemolyticum type strain (11018). Stand Genomic Sci 3:126–135PubMedCentralPubMedCrossRefGoogle Scholar
  26. Yassin AF, Hupfer H, Siering C, Schumann P (2011) Comparative chemotaxonomic and phylogenetic studies on the genus Arcanobacterium Collins et al., 1982 emend Lehnen et al., 2006: proposal for Trueperella gen. nov. and emended description of the genus Arcanobacterium. Int J Syst Evol Microbiol 61:1265–1274PubMedCrossRefGoogle Scholar

Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i. 2014

Authors and Affiliations

  • Osama Sammra
    • 1
  • Alice Friis-Møller
    • 2
  • Anna Balbutskaya
    • 1
  • Muaz Hijazin
    • 1
  • Samy Nagib
    • 1
  • Jörg Alber
    • 1
  • Christoph Lämmler
    • 1
    Email author
  • Amir Abdulmawjood
    • 3
  • Markus Timke
    • 4
  • Markus Kostrzewa
    • 4
  • Ellen Prenger-Berninghoff
    • 5
  1. 1.Institut für Pharmakologie und ToxikologieJustus-Liebig-Universität GießenGießenGermany
  2. 2.Department of Clinical MicrobiologyUniversity HospitalHvidovreDenmark
  3. 3.Institut für Lebensmittelqualität und -sicherheitStiftung Tierärztliche Hochschule HannoverHannoverGermany
  4. 4.Entwicklung BioanalyseBruker Daltonik GmbHBremenGermany
  5. 5.Institut für Hygiene und Infektionskrankheiten der TiereJustus-Liebig-UniversitätGießenGermany

Personalised recommendations