Folia Microbiologica

, Volume 57, Issue 6, pp 501–508 | Cite as

Structural characterization and surface activities of biogenic rhamnolipid surfactants from Pseudomonas aeruginosa isolate MN1 and synergistic effects against methicillin-resistant Staphylococcus aureus

  • Nasrin SamadiEmail author
  • Neda Abadian
  • Reza Ahmadkhaniha
  • Farzaneh Amini
  • Dina Dalili
  • Noushin Rastkari
  • Eliyeh Safaripour
  • Farzaneh Aziz Mohseni


The aim of present work was to study chemical structures and biological activities of rhamnolipid biosurfactants produced by Pseudomonas aeruginosa MN1 isolated from oil-contaminated soil. The results of liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis revealed that total rhamnolipids (RLs) contained 16 rhamnolipid homologues. Di-lipid RLs containing C10-C10 moieties were by far the most predominant congeners among mono-rhamnose (53.29 %) and di-rhamnose (23.52 %) homologues. Mono-rhamnolipids form 68.35 % of the total congeners in the RLs. Two major fractions were revealed in the thin layer chromatogram of produced RLs which were then purified by column chromatography. The retardation factors (R f) of the two rhamnolipid purple spots were 0.71 for RL1 and 0.46 for RL2. LC-MS/MS analysis proved that RL1 was composed of mono-RLs and RL2 consisted of di-RLs. RL1 was more surface-active with the critical micelle concentration (CMC) value of 15 mg/L and the surface tension of 25 mN/m at CMC. The results of biological assay showed that RL1 is a more potent antibacterial agent than RL2. All methicillin-resistant Staphylococcus aureus (MRSA) strains were inhibited by RLs that were independent of their antibiotic susceptibility patterns. RLs remarkably enhanced the activity of oxacillin against MRSA strains and lowered the minimum inhibitory concentrations of oxacillin to the range of 3.12–6.25 μg/mL.


Critical Micelle Concentration Mineral Salt Medium Oxacillin Surface Tension Measurement Biosurfactant Production 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



Colony-forming unit


Critical micelle concentration


Fractional inhibitory concentration


Liquid chromatography-mass spectrometry


Mueller–Hinton broth


Minimum inhibitory concentration


Methicillin-resistant Staphylococcus aureus


Mineral salts medium


Persian type culture collection


Retardation factor




Total ion chromatogram


Thin layer chromatography



This study was supported by a grant from Tehran University of Medical Sciences (no. 5664).


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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i. 2012

Authors and Affiliations

  • Nasrin Samadi
    • 1
    Email author
  • Neda Abadian
    • 1
  • Reza Ahmadkhaniha
    • 2
  • Farzaneh Amini
    • 1
  • Dina Dalili
    • 1
  • Noushin Rastkari
    • 3
  • Eliyeh Safaripour
    • 4
  • Farzaneh Aziz Mohseni
    • 5
  1. 1.Department of Drug and Food Control and Biotechnology Research Center, Faculty of PharmacyTehran University of Medical SciencesTehranIran
  2. 2.Pharmaceutical Sciences Research CenterTehran University of Medical SciencesTehranIran
  3. 3.Institute for Environmental ResearchTehran University of Medical SciencesTehranIran
  4. 4.Pharmaceutical Quality Assurance Research Center, Faculty of PharmacyTehran University of Medical SciencesTehranIran
  5. 5.Biotechnology DepartmentIranian Research Organization for Science and Technology (IROST)TehranIran

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