Bactericidal effect of hydrolysable and condensed tannin extracts on Campylobacter jejuni in vitro
Strategies are sought to reduce intestinal colonisation of food-producing animals by Campylobacter jejuni, a leading bacterial cause of human foodborne illness worldwide. Presently, we tested the antimicrobial activity of hydrolysable-rich blackberry, cranberry and chestnut tannin extracts and condensed tannin-rich mimosa, quebracho and sorghum tannins (each at 100 mg/mL) against C. jejuni via disc diffusion assay in the presence of supplemental casamino acids. We found that when compared to non-tannin-treated controls, all tested tannins inhibited the growth of C. jejuni and that inhibition by the condensed tannin-rich mimosa and quebracho extracts was mitigated in nutrient-limited medium supplemented with casamino acids. When tested in broth culture, both chestnut and mimosa extracts inhibited growth of C. jejuni and this inhibition was much greater in nutrient-limited than in full-strength medium. Consistent with observations from the disc diffusion assay, the inhibitory activity of the condensed tannin-rich mimosa extracts but not the hydrolysable tannin-rich chestnut extracts was mitigated by casamino acid supplementation to the nutrient-limited medium, likely because the added amino acids saturated the binding potential of the condensed tannins. These results demonstrate the antimicrobial activity of various hydrolysable and condensed tannin-rich extracts against C. jejuni and reveal that condensed tannins may be less efficient than hydrolysable tannins in controlling C. jejuni in gut environments containing high concentrations of amino acids and soluble proteins.
- Denny J, Boelaert F, Borck B, Heuer OE, Ammon A, Makela P (2007) Zoonotic infections in Europe: trends and figures - a summary of the EFSA-ECDC annual report. Euro Surveill 12:pii=3336 (2007) Available online: http://www.eurosurveillance.org/ViewArticle.aspx?ArticleId=3336
- Gutierrez-Bañuelos H, Pinchak WE, Min BR, Carstens GE, Anderson RC, Tedeschi LO, Krueger WK, Krueger NA, Lancaster PA, Gomez RR (2011) Effects of feed-supplementation and hide-spray application of two sources of tannins on enteric and hide bacteria of feedlot cattle. J Environ Sci Health B 46:360–365PubMedCrossRefGoogle Scholar
- Krueger WK, Gutierrez-Bañuelos H, Carstens GE, Min BR, Pinchak WE, Anderson RC, Krueger NA, Forbes TDA (2010) Effects of dietary tannin source on performance, feed efficiency, ruminal fermentation, and carcass and non-carcass traits in steers fed a high-grain diet. Anim Feed Sci Technol 159:1–9CrossRefGoogle Scholar
- McSweeney CS, Palmer B, McNeill DM, Krause DO (2001) Microbe interactions with tannins: nutritional consequences for ruminants. Anim Feed Sci Technol 91:83–93Google Scholar
- Min BR, Pinchk WE, Merkel R, Walker S, Tomita G, Anderson RC (2008) Comparative antimicrobial activity of tannin extracts from perennial plants on mastitis pathogens. Sci Res Essays 3:66–73Google Scholar
- Velayudhan J, Kelly DJ (2001) Analysis of gluceoneogenic and anaplerotic enzymes in Campylobacter jejuni: an essential role for phosphoenolpyruvate carboxykinase. Microbiology 148:685–694Google Scholar