Screening of Staphylococcus aureus nasal strains isolated from medical students for toxin genes
- First Online:
Three hundred twenty-one students (156 students with no clinical exposure and 165 students with clinical exposure) were screened for nasal colonization by Staphylococcus aureus; 20.9% of students were S. aureus nasal carriers, and 40.3% of S. aureus isolates harbored toxin genes. The most prevalent genes were tst (15.0 %) and sec (13.4 %). Isolates with multiple genes were only found among clinical students (p = 0.045). Six of 11 PFGE clones were positive for toxin genes. Methicillin-resistant (MRSA) isolates were only detected in the clinical students (4.5 %). The exposure of students to the hospital environment neither radically increased S. aureus nasal carriage, nor the frequency of clinically important toxin gene presence, but it could have influenced the positive selection of toxigenic MRSA strains.
- Bohach GA, Foster TJ (2000) Staphylococcus aureus exotoxins. In: Fischetti VA, Novick RP, Ferretti JJ, Portnoy DA, Rood JI (eds) Gram positive pathogens. ASM, Washington, DC, pp 367–378Google Scholar
- Hryniewicz W, Sulikowska A, Szczypa K, Gniadkowski M, Skoczyńska A (2001) Recommendations for susceptibility testing to antimicrobial agents of selected bacterial species. Mikrobiol Med 12:3–14Google Scholar
- National Committee for Clinical Laboratory Standards (NCCLS) (1994) Performance standards for antimicrobial disk susceptibility tests, 5th edn, Approwed standard M2-A5. National Committee for Clinical Laboratory Standards, WayneGoogle Scholar
- van der Mee-Marquet N, Lina G, Quentin R, Yaouanc-Lapalle H, Fievre C (2003) Staphylococcal exanthematous disease in a newborn due to a virulent methicillin-resistant Staphylococcus aureus strain containing the TSST-1 gene in Europe: an alert for neonatologists. J Clin Microbiol 41:4883–4884PubMedCrossRefGoogle Scholar