Folia Microbiologica

, Volume 56, Issue 1, pp 13–17 | Cite as

Fe/Mn superoxide dismutase-encoding gene in Paracoccus denitrificans is induced by azide and expressed independently of the FNR-type regulators

  • P. Bouchal
  • T. Vyhlídalová
  • I. Struhárová
  • Z. Zdráhal
  • I. KučeraEmail author


Paracoccus denitrificans cells undergo changes in protein composition upon exposure to azide, a known activator of the fumarate–nitrate reduction (FNR)-type transcription factor NarR. One of the most prominent protein species inducible by azide is a Fe/Mn-family superoxide dismutase (SOD). Azide induces SOD at protein, mRNA transcript, and enzyme activity levels in the aerobically growing cells. Since SOD expression remains unaffected in the fnrP-, nnr-, and narR-mutant strains, we postulate a mechanism independent of the known FNR-type regulators but involving a redox signal arising from the respiratory chain.


Azide Nitrate Reductase Terminal Oxidase Paracoccus Denitrificans Superoxide Free Radical 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The authors thank Dr. R. van Spanning (Vrije Universiteit, Amsterdam) who kindly provided with the FnrP-, NNR-, and NarR-mutant strains. The work was supported by grants nos. 203/07/P0471 and P304/10/0868 of the Czech Science Foundation and by the Ministry of Education, Youth and Sports of the Czech Republic (project MSM0021622413).


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Copyright information

© Institute of Microbiology, v.v.i, Academy of Sciences of the Czech Republic 2011

Authors and Affiliations

  • P. Bouchal
    • 1
  • T. Vyhlídalová
    • 1
  • I. Struhárová
    • 1
  • Z. Zdráhal
    • 2
  • I. Kučera
    • 1
    Email author
  1. 1.Department of Biochemistry, Faculty of ScienceMasaryk UniversityBrnoCzech Republic
  2. 2.Department of Functional Genomics and Proteomics, Faculty of Science and CEITECMasaryk UniversityBrnoCzech Republic

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