Microbial phytase-induced calcium-phosphate precipitation — a potential soil stabilization method
- 205 Downloads
Two hypotheses were tested: (1) microbial dephosphorylation of phytate in the presence of Ca2+ ions will result in the precipitation of hydroxyapatite-like crystals and (2) precipitation of calcium-phosphate crystals on and between sand-like particles can cause cementation. A growing culture of the dimorphic phytase-active yeast Arxula adeninivorans was introduced into a column filled with quartz particles and subsequently a liquid growth medium amended with calcium phytate was pumped through the column resulting in increased strength and stiffness of the quartz particle matrix. Environmental scanning electron microscope analysis combined with energy-dispersive X-ray measurement revealed cementation of the quartz particles by calcium-phosphate crystals. This microbial mineralization process could provide a novel approach to improving the mechanical properties like strength and stiffness of sandy soils.
KeywordsQuartz Particle Calcium Hydroxyapatite Monetite Liquid Growth Medium Calcium Phytate
Centraalbureau voor Schimmelcultures
energy dispersive X-ray (analysis)
environmental scanning electron microscope
Yeast Nitrogen Base
Unable to display preview. Download preview PDF.
- Cosgrove D.J.: Chemistry and biochemistry of inositol polyphosphates. Rev.Pure Appl.Chem.16, 209 (1966).Google Scholar
- Lassen S.F., Breinholt J., Ostergaard P.R., Brugger R., Bischoff A., Wyss M., Fuglsang C.C.: Expression, gene cloning, and characterization of five novel phytases from four basidiomycete fungi: Peniophora lycii, Agrocybe pediades, a Ceriporia sp., and Trametes pubescens. Appl.Environ.Microbiol.67, 4701–4707 (2001).CrossRefPubMedGoogle Scholar