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Folia Microbiologica

, Volume 55, Issue 6, pp 621–624 | Cite as

Microbial phytase-induced calcium-phosphate precipitation — a potential soil stabilization method

  • G. Roeselers
  • M. C. M. Van Loosdrecht
Article

Abstract

Two hypotheses were tested: (1) microbial dephosphorylation of phytate in the presence of Ca2+ ions will result in the precipitation of hydroxyapatite-like crystals and (2) precipitation of calcium-phosphate crystals on and between sand-like particles can cause cementation. A growing culture of the dimorphic phytase-active yeast Arxula adeninivorans was introduced into a column filled with quartz particles and subsequently a liquid growth medium amended with calcium phytate was pumped through the column resulting in increased strength and stiffness of the quartz particle matrix. Environmental scanning electron microscope analysis combined with energy-dispersive X-ray measurement revealed cementation of the quartz particles by calcium-phosphate crystals. This microbial mineralization process could provide a novel approach to improving the mechanical properties like strength and stiffness of sandy soils.

Keywords

Quartz Particle Calcium Hydroxyapatite Monetite Liquid Growth Medium Calcium Phytate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

A.a.

Arxula adeninivorans

CaPh

calcium phytate

CBS

Centraalbureau voor Schimmelcultures

EDX

energy dispersive X-ray (analysis)

ESEM

environmental scanning electron microscope

YNB

Yeast Nitrogen Base

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Copyright information

© Institute of Microbiology, v.v.i, Academy of Sciences of the Czech Republic 2010

Authors and Affiliations

  1. 1.Department of BiotechnologyDelft University of TechnologyDelftThe Netherlands
  2. 2.Biological Laboratory 4081Harvard UniversityCambridgeUSA

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