Folia Microbiologica

, Volume 55, Issue 3, pp 277–280 | Cite as

Impact of heat-inactivated Lactobacillus casei and Lactobacillus paracasei strains on cytokine responses in whole blood cell cultures of children with atopic dermatitis

  • B. CukrowskaEmail author
  • I. Rosiak
  • E. Klewicka
  • I. Motyl
  • M. Schwarzer
  • Z. Libudzisz
  • H. Kozakova


Heat-inactivated Lactobacillus casei LOCK 0900, L. casei LOCK 0908 and Lactobacillus paracasei LOCK 0919 strains, applied to blood cell cultures obtained from children with atopic dermatitis induced production of anti-allergic TH1 cytokines (interleukin-12, interleukin-18, interferon-γ, tumor necrosis factor-α) and regulatory transforming growth factor-β1), but did not stimulate pro-allergic interleukin-5. The lactobacilli-mixture remarkably enhanced the TH1 response compared to single strains. This synergistic effect was not observed for transforming growth factor-β1. In contrast, the amount of interleukin-10 was found to be considerably lower when cells were stimulated with lactobacilli-mixture compared to single strains. The mixture of Lactobacillus strains represents a probiotic bacterial preparation modulating in vitro cytokine profile of allergic children towards anti-allergic TH1 response.


Lactobacillus Atopic Dermatitis Lactobacillus Strain Lactobacillus Paracasei Allergic Child 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.







phosphate-buffered saline




transforming growth factor


T helper (lymphocytes)


tumor necrosis factor


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Copyright information

© Institute of Microbiology, v.v.i, Academy of Sciences of the Czech Republic 2010

Authors and Affiliations

  • B. Cukrowska
    • 1
    Email author
  • I. Rosiak
    • 1
  • E. Klewicka
    • 2
  • I. Motyl
    • 2
  • M. Schwarzer
    • 3
  • Z. Libudzisz
    • 2
  • H. Kozakova
    • 3
  1. 1.Department of PathologyThe Children’s Memorial Health InstituteWarsawPoland
  2. 2.Institute of Fermentation Technology and MicrobiologyTechnical University of LodzLodzPoland
  3. 3.Institute of MicrobiologyAcademy of Sciences of the Czech RepublicNovy HradekCzech Republic

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