Interactions of antibiotics and extracts of Helichrysum pedunculatum against bacteria implicated in wound infections
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The effect of combinations of the crude acetone and aqueous extracts of Helichrysum pedunculatum leaves and eight antibiotics was determined by means of checkerboard and time-kill methods. In the checkerboard method, synergy of 45.8 % was observed, being independent of Gram reaction, with combinations in the aqueous extract yielding largely (18.8 %) antagonistic interactions. The time-kill assay detected synergy (45.8 %) that was also independent of Gram reaction with a potentiation of more than 3 orders of the bactericidal activity of the test antibiotics. The crude leaf extracts of H. pedunculatum could thus be considered to be potential source of a broad-spectrum antibiotic-resistance-modifying compounds.
KeywordsGatifloxacin EUCAST Fractional Inhibitory Concentration Fractional Inhibitory Concentration Index Crude Leaf Extract
fractional inhibitory concentration
multi drug resistance
minimum inhibitory concentration
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