Folia Microbiologica

, 54:429 | Cite as

Changes in the incidence of periodontal pathogens during long-term monitoring and after application of antibacterial drugs

  • T. Janatová
  • L. Najmanová
  • L. Neubauerová
  • M. Kyselková
  • G. Novotná
  • J. Spížek
  • J. Janata
  • J. Dušková
Article
First Online:
Received:
Revised:

Abstract

The incidence of potential periodontal pathogens (Aggregatibacter actinomycetemcomitans, formerly Actinobacillus actinomycetemcomitans, Tannerella forsythia, Porphyromonas gingivalis, Prevotella nigrescens, Prevotella intermedia and Capnocytophaga ochracea) was monitored in patients with chronic periodontitis and in healthy control subjects. Two types of studies were carried out in which the composition of the bacterial communities in different niches of the same oral cavity ecosystem was investigated. Fluctuation or at least pronounced quantitative changes in the incidence of individual species in time were documented in the long-term study as well as after the local administration of antibacterial drug Chlo-Site or Metronidazole. Even within two weeks, a turnover of the monitored bacteria in separate niches of the oral biotope can be detected. A relatively high incidence of the tested periopathogens in the clinically healthy teeth of patients implies that even the “healthy” niches in the periodontal biotope function as a dynamic reservoir of periopathogenic microorganisms. This should be kept in mind when a local application of antibacterial compounds is used in the therapy of periodontal disease.

Keywords

Periodontitis Chronic Periodontitis Streptococcus Mutans Porphyromonas Gingivalis Periodontal Pocket 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
This is a preview of subscription content, log in to check access.

References

  1. American Academy of Periodontology: Consensus report: section on epidemiology. Ann.Periodontol. 1, 216–218 (1996).Google Scholar
  2. Ashelford K.E., Weightman A.J., Fry J.C.: Primrose: a computer program for generating and estimating the phylogenetic range of 16S rDNA oligonucleotide probes and primers in conjunction with the RDP-II database. Nucl.Acids Res. 30, 3481–3489 (2002).CrossRefPubMedGoogle Scholar
  3. Ashimoto A., Chen C., Bakker I., Slots J.: Polymerase chain reaction detection of 8 putative periodontal pathogens in subgingival plaque of gingivitis and advanced periodontitis lesions. Oral Microbiol.Immunol. 11, 266–273 (1996).CrossRefPubMedGoogle Scholar
  4. Black C., Allan I., Ford S.K., Wilson M., Mcnab R.: Biofilm-specific surface properties and protein expression in oral Streptococcus sanguis. Arch.Oral Biol. 49, 295–304 (2004).CrossRefPubMedGoogle Scholar
  5. Brown L.J., Löe H.: Prevalence, extent, severity and progression of periodontal disease. Periodontology 2000 2, 57–71 (1993).CrossRefPubMedGoogle Scholar
  6. Choi B.K., Paster B.J., Dewhirst F.E., Gobel U.B.: Diversity of cultivable and uncultivable oral spirochetes from a patient with severe destruction periodontitis. Infect.Immun. 62, 1889–1895 (1994).PubMedGoogle Scholar
  7. Eckert R., He J., Yarbrough D.K., Qi F., Anderson M.H., Shi W.: Targeted killing of Streptococcus mutans by a pheromone-guided “Smart” antimicrobial peptide. Antimicrob.Agents Chemother. 50, 3651–3657 (2006).CrossRefPubMedGoogle Scholar
  8. Fine D.H., Furgang D., Barnett M.L.: Comparative antimicrobial activities of antiseptic mouthrinses against isogenic planktonic and biofilm forms of Actinobacillus actinomycetemcomitans. J.Clin.Periodontol. 28, 697–700 (2001).CrossRefPubMedGoogle Scholar
  9. Haffajee A.D., Socransky S.S.: Microbial etiological agents of destructive periodontal diseases. Periodontology 2000 10, 78–111 (1996).Google Scholar
  10. Kara D., Luppens S.B.I., Cate J.M.: Differences between single- and dual-species biofilms of Streptococcus mutans and Veillonella parvula in growth, acidogenicity and susceptibility to chlorhexidine. Eur.J.Oral Sci. 114, 58–63 (2006).CrossRefPubMedGoogle Scholar
  11. Kara D., Luppens S.B.I., van Marle J., Ozok R., ten Cate J.M.: Microstructural differences between single-species and dual-species biofilms of Streptococcus mutans and Veillonella parvula, before and after exposure to chlorhexidine. FEMS Microbiol. Lett. 271, 90–97 (2007).CrossRefPubMedGoogle Scholar
  12. Kuramitsu H.K., He X.S., Lux R., Anderson M.H., Shi W.Y.: Interspecies interactions within oral microbial communities. Microbiol.Mol.Biol.Rev. 71, 653–670 (2007).CrossRefPubMedGoogle Scholar
  13. Mercuri L.G.: Microbial biofilms: a potential source for alloplastic device failure. J.Oral Maxillofac.Surg. 64, 1303–1309 (2006).CrossRefPubMedGoogle Scholar
  14. Nishihara T., Koseki T: Microbial etiology of periodontitis. Periodontology 2000 36, 14–26 (2004).CrossRefPubMedGoogle Scholar
  15. Sanz M., Lau L., Herrera D., Morillo M.J., Silva A.: Methods of detection of Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis and Tannerella forsythensis in periodontal microbiology, with special emphasis on advanced molecular techniques: a review. J.Clin.Periodontol. 31, 1034–1047 (2004).CrossRefPubMedGoogle Scholar
  16. Tran S.D., Rudney J.D.: Improved multiplex PCR using conserved and species-specific 16S rRNA gene primers for simultaneous detection of Actinobacillus actinomycetemcomitans, Bacteroides forsythus, and Porphyromonas gingivalis. J.Clin.Microbiol. 37, 3504–3508 (1999).PubMedGoogle Scholar
  17. van Winkelhoff A.J., Loos B.G., van der Reijden W.A., van Der Velden U.: Porphyromonas gingivalis, Bacteroides forsythus and other putative periodontal pathogens in subjects with and without periodontal destruction. J.Clin.Periodontol. 29, 1023–1028 (2002).CrossRefPubMedGoogle Scholar

Copyright information

© Institute of Microbiology, v.v.i, Academy of Sciences of the Czech Republic 2009

Authors and Affiliations

  • T. Janatová
    • 1
  • L. Najmanová
    • 2
  • L. Neubauerová
    • 2
  • M. Kyselková
    • 2
  • G. Novotná
    • 2
  • J. Spížek
    • 2
  • J. Janata
    • 2
  • J. Dušková
    • 1
  1. 1.Institute of Dental Research, 1st Faculty of Medicine and General Faculty HospitalCharles University in PraguePragueCzech Republic
  2. 2.Institute of Microbiology, v.v.i.Academy of Sciences of the Czech RepublicPragueCzech Republic

Personalised recommendations