Advertisement

Folia Microbiologica

, 54:327 | Cite as

The impact of zinc and lead concentrations and seasonal variation on bacterial and actinobacterial community structure in a metallophytic grassland soil

  • L. Bamborough
  • S. P. CummingsEmail author
Article

Abstract

The diversity and structure of bacterial and actinobacteral diversity and communities were determined in a metallophytic grassland soil from an upland site in northern England. The community profiles were subjected to multivariate analyses using correspondence and cluster analyses. The total bacterial community diversities and structures were not significantly affected by Pb and Zn concentration in the soil. However, the community structure did show changes between winter and summer samples. Raup and Crick analysis indicated that deterministic selection lead to winter profiles exhibiting significant similarity. The actinobacterial community was also unaffected by Pb and Zn concentration. However, seasonal changes were apparent as diversity were significantly lower in winter compared to summer profiles. Moreover, the community structure showed evidence of changes of structure based on the seasonal samples with winter samples showing significant similarity to each other.

Keywords

Microbial Community Bacterial Community Bacterial Community Structure Summer Sample Contamination Index 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

CA

correspondence analysis

DGGE

denaturing gradient gel electrophoresis

CI

contamination index

RCA

Raup and Crick analysis

References

  1. Abaye D.A., Lawlor K., Hirsch P.R., Brookes P.C.: Changes in the microbial community of an arable soil caused by long-term metal contamination. Eur.J.Soil Sci. 56, 93–102 (2005).CrossRefGoogle Scholar
  2. Bååth E., Diaz-ravina M., Bakken L.R.: Microbial biomass, community structure and metal tolerance of a naturally Pb-enriched forest soil. Microb.Ecol. 50, 496–505 (2005).CrossRefPubMedGoogle Scholar
  3. Balabane M., Faivre D., Van Oort F., Dahmani-Muller H.: Mutual effects of soil organic matter dynamics and heavy metals fate in a metallophytic grassland. Environ.Poll. 105, 45–54 (1999).CrossRefGoogle Scholar
  4. Bamborough L., Cummings S.P.: The impact of increasing heavy metal stress on the diversity and structure of the bacterial and actinobacterial communities of metallophytic grassland soil. Biol.Fert.Soil 45, 273–280 (2009).CrossRefGoogle Scholar
  5. Baxter J., Cummings S.P.: The impact of bioaugmentation on metal cyanide degradation and soil bacteria community structure. Biodegradation 17, 207–217 (2006).CrossRefPubMedGoogle Scholar
  6. Brodie E., Edwards S., Clipson N.: Soil fungal community structure in a temperate upland grassland soil. FEMS Microbiol.Ecol. 45, 105–114 (2003).CrossRefPubMedGoogle Scholar
  7. Feris K., Ramsey P., Frazar C., Moore J.N., Gannon J.E., Holbert W.E.: Differences in hyporheic-zone microbial community structure along a heavy-metal contamination gradient. Appl.Environ.Microbiol. 69, 5563–5573 (2003).CrossRefPubMedGoogle Scholar
  8. Feris K.P., Ramsey P.W., Rillig M., Moore J.N., Gannon J.E., Holbert W.E.: Determining rates of change and evaluating group-level resiliency differences in hyporheic microbial communities in response to fluvial heavy-metal deposition. Appl.Environ. Microbiol. 70, 4756–4765 (2004).CrossRefPubMedGoogle Scholar
  9. Frey B., Stemmer M., Widmer F., Luster J., Sperisen C.: Microbial activity and community structure of a soil after heavy metal contamination in a model forest ecosystem. Soil Biol.Biochem. 38, 1745–1756 (2006).CrossRefGoogle Scholar
  10. Giller K.E., Witter E., Mcgrath S.P.: Toxicity of heavy metals to microorganisms and microbial processes in agricultural soils; a review. Soil Biol.Biochem. 30, 1389–1414 (1998).CrossRefGoogle Scholar
  11. Grayston S.J., Griffith G.S., Mawdsley C.D., Campbell C.D., Bardgett R.D.: Accounting for variability in soil microbial communities of temperate upland grassland ecosystems. Soil Biol.Biochem. 33, 533–551 (2001).CrossRefGoogle Scholar
  12. Gremion F., Chatzinotas A., Harms H.: Comparative 16S rDNA and 16S rRNA sequence analysis indicates that Actinobacteria might be a dominant part of the metabolically active bacteria in heavy metal-contaminated bulk and rhizosphere soil. Environ. Microbiol. 5, 896–907 (2003).CrossRefPubMedGoogle Scholar
  13. Griffiths B.S., Bonkowski M., Roy J., Ritz K.: Functional stability, substrate utilisation and biological indicators of soils following environmental impacts. Appl.Soil Ecol. 16, 49–61 (2001).CrossRefGoogle Scholar
  14. Griffiths R.I., Whitely A.S., O’Donnell A.G., Bailey M.J.: Influence of depth and sampling time on bacterial community structure in an upland grassland soil. FEMS Microbiol.Ecol. 43, 35–43 (2003).CrossRefPubMedGoogle Scholar
  15. Griffiths B.S., Hallett P.D., Kuan H.L., Pitkin Y., Aitken M.N.: Biological and physical resilience of soil amended with heavy metal-contaminated sewage sludge. Europ.J.Soil Sci. 56, 197–205 (2005).CrossRefGoogle Scholar
  16. Hery M., Nazaret S., Jaffre T., Normand P., Navarro E.: Adaptation to nickel spiking of bacterial communities in neocaledonian soils. Environ.Microbiol. 5, 3–12 (2003).CrossRefPubMedGoogle Scholar
  17. Heuer H., Krsek M., Baker P., Smalla K., Wellington E.M.H.: Analysis of actinomycete communities by specific amplification of genes encoding 16S rRNA and gel-electrophoretic separation in denaturing gradients. Appl.Environ.Microbiol. 63, 3233–3241 (1997).PubMedGoogle Scholar
  18. Holmes A.J., Bowyer J., Holley M.P., O’Donoghue M., Montgomery M., Gillings M.R.: Diverse, yet-to-be-cultured members of the Rubrobacter subdivision of the Actinobacteria are widespread in Australian arid soils. FEMS Microbiol.Ecol. 33, 111–120 (2000).CrossRefPubMedGoogle Scholar
  19. Intawongse M., Dean J.R.: Use of the physiologically-based extraction test to assess the oral bioaccessibility of metals in vegetable plants grown in contaminated soil. Environ.Poll. 152, 60–72 (2008).CrossRefGoogle Scholar
  20. Joynt J., Bischoff M., Turco R., Konopka A., Nakatsu C.H.: Microbial community analysis of soils contaminated with lead, chromium and petroleum hydrocarbons. Microb.Ecol. 51, 209–219 (2006).CrossRefPubMedGoogle Scholar
  21. Kennedy N.M., Gleeson D.E., Connolly J., Clipson N.J.W.: Seasonal and management influences on bacterial community structure in an upland grassland soil. FEMS Microbiol.Ecol. 53, 329–337 (2005).CrossRefPubMedGoogle Scholar
  22. Li Z.J., Xu J.M. Tang C.X., Wu J.J., Muhammad A., Wang H.Z.: Application of 16S rDNA-PCR amplification and DGGE fingerprinting for detection of shift in microbial community diversity in Cu-, Zn-, and Cd-contaminated paddy soils. Chemosphere 62, 1374–1380 (2006).CrossRefPubMedGoogle Scholar
  23. Lorenz N., Hintemann T., Kramarewa T., Katayama A., Yasuta T., Marschner P., Kandeler E.: Response of microbial activity and microbial community composition in soils to long-term arsenic and cadmium exposure. Soil Biol.Biochem. 38, 1430–1437 (2006).CrossRefGoogle Scholar
  24. Mummey D.L., Stahl P.D.: Analysis of soil whole- and inner-microaggregate bacterial communities. Microb.Ecol. 48, 41–50 (2004).CrossRefPubMedGoogle Scholar
  25. Muyzer G., DE Waal E.C., Uitterlinden A.G.: Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl.Environ.Microbiol. 59, 695–700 (1993).PubMedGoogle Scholar
  26. Ogino A., Koshikawa H., Nakahara T., Uchiyama H.: Succession of microbial communities during a biostimulation process as evaluated by DGGE and clone library analyses. J.Appl.Microbiol. 91, 625–635 (2001).CrossRefPubMedGoogle Scholar
  27. Quevauviller P.: Operationally defined extraction procedures for soil and sediment analysis — II. Certified reference materials. Trends Analyt.Chem. 17, 632–642 (1998).CrossRefGoogle Scholar
  28. Ramette A.: Multivariate analyses in microbial ecology. FEMS Microbiol.Ecol. 62, 142–160 (2007).CrossRefPubMedGoogle Scholar
  29. Rowan A.K., Snape J.R., Fearnside D., Barer M.R., Curtis T.P., Head I.M.: Composition and diversity of ammonia-oxidising bacterial communities in wastewater treatment reactors of different design treating identical wastewater. FEMS Microbiol.Ecol. 43, 195–206 (2003).CrossRefPubMedGoogle Scholar
  30. Sigler W.V., Turco R.F.: The impact of chlorothalonil application on soil bacterial and fungal populations as assessed by denaturing gradient gel electrophoresis. Appl.Soil Ecol. 21, 107–118 (2002).CrossRefGoogle Scholar

Copyright information

© Institute of Microbiology, v.v.i, Academy of Sciences of the Czech Republic 2009

Authors and Affiliations

  1. 1.Biomolecular and Biomedical Research Centre, School of Applied SciencesNorthumbria UniversityNewcastle-upon-TyneUK

Personalised recommendations