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Folia Microbiologica

, Volume 53, Issue 6, pp 521–526 | Cite as

Staphylococcus cohnii hemolysins — Isolation, purification and properties

  • M. Różalska
  • E. M. Szewczyk
Article

Abstract

A total 355 of Staphylococcus cohnii isolates from hospital environment, patients (newborns), medical staff and from non-hospital environment were tested for hemolytic activity. Ninety-one % of S. cohnii ssp. cohnii and 74.5 % S. cohnii ssp. urealyticus strains exhibited hemolysis synergistic to S. aureus ATCC 25923 strain. Crude preparations of hemolysins of both bacterial subspecies presented δ-hemolysin, but not α- and β-toxin activity. Highly pure hemolysins were obtained by semipreparative SDS-PAGE or by organic solvent extraction from the freeze-dried crude preparations. Native-PAGE and 2D-PAGE showed their high heterogeneity. Molar masses of single hemolysin units estimated by the Tris-Tricine-SDS-PAGE were calculated as 3.47 kDa for S. cohnii ssp. cohnii and 3.53 kDa for S. cohnii ssp. urealyticus.

Keywords

Hemolytic Activity Tricine Hospital Environment Streptococcus Agalactiae Crude Preparation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

“BAS”

“blood agar sandwich”

BLIS

bacteriocin-like substances

CNS

coagulase-negative staphylococci

ICU

Intensive Care Units

PBS

phosphate-buffered solution

Scc

Staphylococcus cohnii ssp. cohnii

Scu

Staphylococcus cohnii ssp. urealyticus

SRBC

sheep red blood cells

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Copyright information

© Institute of Microbiology, v.v.i, Academy of Sciences of the Czech Republic 2008

Authors and Affiliations

  1. 1.Department of Pharmaceutical MicrobiologyMedical University of ŁódźŁódźPoland

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