Folia Microbiologica

, 53:417 | Cite as

Identification of a new molecular target of class IIa bacteriocins in Listeria monocytogenes EGDe

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Abstract

It was shown recently (Calvez et al. 2007) that glpQ and pde genes are involved in intermediate resistance of Enterococcus faecalis JH2-2 to DvnV41, a class IIa bacteriocin produced by Carnobacterium divergens V41. The listerial orthologs of lpQ and pde genes might be lmo0052 and lmo1292 genes, respectively. Here, the role of lmo0052 and lmo1292 genes in resistance of Listeria monocytogenes EGDe to DvnV41 and MesY105 was investigated. L. monocytogenes EGDe was inactivated in lmo0052 and/or lmo1292 by homologous recombination. Listerial mutant strain EGDSC02 (inactivated in the putative glpQ gene), was slightly resistant to DvnV41. The listerial mutant strain EGDSC01 (inactivated in the putative pde gene) remained, as the wild-type strain, sensitive to DvnV41, but was affected in growth parameters.

Keywords

Mutant Strain Listeria Monocytogenes Intermediate Resistance NcoI Restriction Site rpoN Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

BHI

brain heart infusion

MRS

de Man-Rogosa-Sharpe

Dvn V41

divercin V41

ORF(s)

open reading frame(s)

LB

Luria-Bertani

PTS

phosphotransferase system

Mes Y105

mesentericin Y105

X-Gal

5-bromo-4-chloro-3-indolyl β-d-galactopyranoside

MIC

minimum inhibitory concentration

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Copyright information

© Institute of Microbiology, v.v.i, Academy of Sciences of the Czech Republic 2008

Authors and Affiliations

  1. 1.Laboratoire de MicrobiologieEcole Nationale d’Ingénieurs des Techniques des Industries Agricoles et AlimentairesNantes Cedex 3France

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