Folia Microbiologica

, 53:357 | Cite as

Multiplex PCR detection of the antibiotic resistance genes in Staphylococcus aureus strains isolated from auricular infections

  • T. Zmantar
  • K. Chaieb
  • F. Ben Abdallah
  • A. Ben Kahla-Nakbi
  • A. Ben Hassen
  • K. Mahdouani
  • A. Bakhrouf
Papers

Abstract

Thirty-five Staphylococcus aureus strains from auricular infections were isolated. The identification of strains was confirmed by Api ID 32 Staph strips, the antibiotic susceptibility test was performed using ATB Staph kit. PCR assay was used to detect the oxacillin resistance gene (mecA) and the erythromycin genes (ermA, ermB, ermC, msrA and mef). The susceptibility profile of all strains revealed a low resistance level to oxacillin and erythromycin. The PCR results show that 60 % of the strains are mecA positive. The frequency of erythromycin genes was: ermA+ 22.8 %, ermB+ 45.7, ermC+ 17.1, msrA+ 28.6. The mef gene was not detected in any strain. No correlations between genotypic and phenotypic methods for the determination of oxacillin and erythromycin resistance was found. However, multiplex PCR technique was shown to be a fast, practical and economic technique for the detection of methicillin-and erythromycin-resistant staphylococci.

Abbreviations

ERM

erythromycin

PCR

polymerase chain reaction

MRSA

methicillin-resistant S. aureus

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Copyright information

© Institute of Microbiology, v.v.i, Academy of Sciences of the Czech Republic 2008

Authors and Affiliations

  • T. Zmantar
    • 1
  • K. Chaieb
    • 1
    • 2
  • F. Ben Abdallah
    • 1
  • A. Ben Kahla-Nakbi
    • 1
  • A. Ben Hassen
    • 3
  • K. Mahdouani
    • 2
  • A. Bakhrouf
    • 1
  1. 1.Laboratoire d’Analyse, Traitement et Valorisation des Polluants de l’Environnement et des ProduitsFaculté de PharmacieMonastirTunisie
  2. 2.Laboratoire de Bactériologie de l’Hôpital Ibn El Jazzar de KairouanKairouanTunisie
  3. 3.Centre de Greffe de Moelle OsseuseTunisTunisie

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