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Folia Microbiologica

, Volume 53, Issue 3, pp 263–269 | Cite as

Auto-aggregation and Co-aggregation ability in bifidobacteria and clostridia

  • E. Vlková
  • V. Rada
  • M. Šmehilová
  • J. Killer
Article

Abstract

A total of 142 human and 88 calf bifidobacteria were isolated and identified; ≈12 % of all isolated strains exhibited auto-aggregation (Agg) phenotype (Agg+). Properties considered to be predicting for their adhesion to intestine, i.e. auto-aggregation, and hydrophobicity were determined by xylene extraction in 18 human and 8 calf origin bifidobacteria. Co-aggregation of 8 human bifidobacteria with 8 clostridia was also evaluated. Agg varied between 16.3 and 96.4 %, hydrophobicity values ranged from 0 to 82.8 %. The strongest Agg and hydrophobicity were observed in B. bifidum and B. merycicum isolates. However, there were no statistically significant correlations between these two properties. Variability in the percentage of Agg and hydrophobicity was observed after cultivation of bifidobacteria on different carbon sources. All bifidobacteria showed co-aggregation ability with clostridia tested but there were remarkable differences depending on specific combinations of strains. The bifidobacterial strains with the highest ability to co-aggregate with clostridia were B. bifidum I4 and B. longum I10 isolated from infants; these strains gave also high values of Agg. Agg properties together with co-aggregation ability with potential pathogen can be used for preliminary selection of probiotic bacteria.

Keywords

Lactic Acid Bacterium Probiotic Bacterium Probiotic Strain Cell Surface Hydrophobicity Intestinal Mucus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Institute of Microbiology, v.v.i, Academy of Sciences of the Czech Republic 2008

Authors and Affiliations

  • E. Vlková
    • 1
  • V. Rada
    • 1
  • M. Šmehilová
    • 1
  • J. Killer
    • 1
  1. 1.Department of Microbiology, Nutrition and DieteticsCzech University of Life Sciences PraguePragueCzechia

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