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Folia Microbiologica

, 53:61 | Cite as

The influence of Lactobacillus acidophilus-derived surfactants on staphylococcal adhesion and biofilm formation

  • E. Walencka
  • S. Różalska
  • B. Sadowska
  • B. RóżalskaEmail author
Article

Abstract

The ability of surfactants obtained from three Lactobacillus acidophilus strains to inhibit Staphylococcus aureus and S. epidermidis biofilms was evaluated. Their influence was determined on bacterial initial adhesion, biofilm formation and dispersal using MTT-reduction assay, confocal laser scanning microscopy and image PHLIP analysis. The number of adhering S. aureus and S. epidermidis cells after a 3-h co-incubation with biosurfactants was reduced by 5–56 % in a strain-and dose-dependent manner. S. epidermidis-and, to a lower extent, in S. aureus-biofilm formation was also inhibited in the presence of the tested surfactants. The addition of surfactants to preformed mature biofilms accelerated their dispersal, and changed the parameters of biofilm morphology. The L. acidophilus-derived surfactants inhibit bacterial deposition rate and biofilm development (and also its maturation) without affecting cell growth probably due to the influence on the cell-surface hydrophobicity of staphylococci.

Keywords

Lactobacillus Surfactin Lactobacillus Acidophilus Antibiofilm Activity Diammonium Sulfate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

LAA

Lactobacillus acidophilus

SAU

Staphylococcus aureus

SEP

Staphylococcus epidermidis

BSA

bovine serum albumin

CFU

colony-forming unit(s)

CLSM

confocal laser scanning microscopy

CSH

cell-surface hydrophobicity

FITC

fluorescein isothiocyanate

MRS

De Man-Rogosa-Sharpe (broth)

MTT

3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide

PBS

phosphate buffered saline

PHLIP

phobia laser scanning microscopy imaging processor

SAT

salt-aggregation test

TSB

tryptic-soy broth

TSBGlc

TSB with 0.25 % d-glucose

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Copyright information

© Institute of Microbiology, v.v.i, Academy of Sciences of the Czech Republic 2008

Authors and Affiliations

  • E. Walencka
    • 1
  • S. Różalska
    • 2
  • B. Sadowska
    • 1
  • B. Różalska
    • 1
    Email author
  1. 1.Department of Immunology and Infectious Biology, Institute of Microbiology and ImmunologyUniversity of ŁódźŁódźPoland
  2. 2.Department of Industrial Microbiology and Biotechnology, Institute of Microbiology and ImmunologyUniversity of ŁódźŁódźPoland

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