Folia Microbiologica

, 53:61 | Cite as

The influence of Lactobacillus acidophilus-derived surfactants on staphylococcal adhesion and biofilm formation

  • E. Walencka
  • S. Różalska
  • B. Sadowska
  • B. RóżalskaEmail author


The ability of surfactants obtained from three Lactobacillus acidophilus strains to inhibit Staphylococcus aureus and S. epidermidis biofilms was evaluated. Their influence was determined on bacterial initial adhesion, biofilm formation and dispersal using MTT-reduction assay, confocal laser scanning microscopy and image PHLIP analysis. The number of adhering S. aureus and S. epidermidis cells after a 3-h co-incubation with biosurfactants was reduced by 5–56 % in a strain-and dose-dependent manner. S. epidermidis-and, to a lower extent, in S. aureus-biofilm formation was also inhibited in the presence of the tested surfactants. The addition of surfactants to preformed mature biofilms accelerated their dispersal, and changed the parameters of biofilm morphology. The L. acidophilus-derived surfactants inhibit bacterial deposition rate and biofilm development (and also its maturation) without affecting cell growth probably due to the influence on the cell-surface hydrophobicity of staphylococci.


Lactobacillus Surfactin Lactobacillus Acidophilus Antibiofilm Activity Diammonium Sulfate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



Lactobacillus acidophilus


Staphylococcus aureus


Staphylococcus epidermidis


bovine serum albumin


colony-forming unit(s)


confocal laser scanning microscopy


cell-surface hydrophobicity


fluorescein isothiocyanate


De Man-Rogosa-Sharpe (broth)


3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide


phosphate buffered saline


phobia laser scanning microscopy imaging processor


salt-aggregation test


tryptic-soy broth


TSB with 0.25 % d-glucose


  1. Bêhal V.: Mode of action of microbial bioactive metabolites. Folia Microbiol. 51, 359–369 (2006).CrossRefGoogle Scholar
  2. Bodour A.A., Miller-Maier R.M.: Application of a modified drop-collapse technique for surfactant quantitation and screening of biosurfactant-producing microorganisms. J.Microbiol.Meth. 32, 273–280 (1998).CrossRefGoogle Scholar
  3. Doyle R.J.: Contribution of the hydrophobic effect to microbial infection. Microb.Infect. 2, 391–400 (2000).CrossRefGoogle Scholar
  4. Espinosa-Urgel M.: Resident parking only: rhamnolipids maintain fluid channels in biofilm. J.Bacteriol. 185, 699–700 (2003).PubMedCrossRefGoogle Scholar
  5. Goetz F.: Staphylococcus and biofilms. Mio.Microbiol. 43, 1367–1378 (2002).CrossRefGoogle Scholar
  6. Hall-Stoodley L., Costerton J.W., Stoodley P.: Bacterial biofilms: from the natural environment to infectious diseases. Nature Rev. 2, 95–106 (2004).CrossRefGoogle Scholar
  7. VAN Hoogmoed C.G., Van Der Kuijl-Booij M., Van Der Mei H.C., Busscher H.J.: Inhibition of Streptococcus mutans NS adhesion to glass with and without a salivary conditioning film by biosurfactant-releasing Streptococcus mitis strains. Appl.Environ.Microbiol. 66, 659–663 (2000).PubMedCrossRefGoogle Scholar
  8. Horošová K., Bujñáková D., Kmeť V.: Effect of lactobacilli on E. coli adhesion to Caco-2 cells in vitro. Folia Microbiol. 51, 281–282 (2006).CrossRefGoogle Scholar
  9. Lewis K.: Riddle of biofilm resistance. Antimicrob.Agents Chemother. 45, 999–1007 (2001).PubMedCrossRefGoogle Scholar
  10. Mireles J.R. II, Toguchi A., Harshey R.M.: Salmonella enterica serovar Typhimurium swarming mutants with altered biofilm-forming abilities: surfactin inhibits biofilm formation. J.Bacteriol. 183, 5848–5854 (2001).PubMedCrossRefGoogle Scholar
  11. Mack D., Becker P., Chatterje I., Dobinsky S., Knobloch J.K.-M., Peter G., Rohde H., Herrmann M.: Mechanisms of biofilm formation in Staphylococus epidermidis and Staphylococcus aureus: functional molecules, regulatory circuits, and adaptive responses. Internat.J.Med.Microbiol. 294, 203–212 (2004).CrossRefGoogle Scholar
  12. Merk K., Borelli C., Korting H.C.: Lactobacilli — bacteria-host interactions with special regard to the urogenital tract. Internat. J.Med.Microbiol. 295, 9–18 (2005).CrossRefGoogle Scholar
  13. Mueller L.N., De Brouwer J.F.C., Almeida J.S., Stal L.J., Xavier J.B.: Analysis of a marine phototrophic biofilm by confocal laser scanning microscopy using the new image quantification software PHLIP. MMC Ecology 6, 1–15 (2006).Google Scholar
  14. Ofek I., Hasty D.L. Sharon N.: Anti-adhesion therapy of bacterial diseases: prospects and problems. FEMS Immunol.Med.Microbiol. 38, 181–191 (2003).PubMedCrossRefGoogle Scholar
  15. Reid G., Burton J.: Use of Lactobacillus to prevent infection by pathogenic bacteria. Microb.Infect. 4, 319–324 (2002).CrossRefGoogle Scholar
  16. Reid G., Tieszer C., Lam D.: Influence of lactobacilli on the adhesion of Staphylococcus aureus and Candida albicans to fibers and epithelial cells. J.Ind.Microbiol. 15, 248–253 (1995).PubMedCrossRefGoogle Scholar
  17. Rodrigues L., Van Der Mei H., Banat I.M., Teixeira J., Oliveira R.: Biosurfactant from Lactococcus lactis 53 inhibits microbial adhesion on silicone rubber. Appl.Genet.Molec.Biotech. 66, 306–311 (2004a).CrossRefGoogle Scholar
  18. Rodrigues L., Van Der Mei H., Banat I.M., Teixeira J., Oliveira R.: Influence of biosurfactants from probiotic bacteria on formation of biofilms on voice prostheses. Appl.Environ.Microbiol. 70, 4408–4410 (2004b).PubMedCrossRefGoogle Scholar
  19. Rodrigues L., Van Der Mei H., Banat I.M., Teixeira J., Oliveira R.: Inhibition of microbial adhesion to silicone rubber treated with biosurfactant from Streptococcus thermophilus A. FEMS Immunol.Med.Microbiol., 46, 107–112 (2006a).PubMedCrossRefGoogle Scholar
  20. Rodrigues L., Banat I.M., Teixeira J., Oliveira R.: Biosurfactants: potential applications in medicine. J.Antimicrob.Chemother. 57, 609–618 (2006b).PubMedCrossRefGoogle Scholar
  21. Růžička F., Holá V., Votava M., Tejkalová R., Horvát R., Heroldová M., Woznicová V.: Biofilm detection and the clinical significance of Staphylococcus epidermidis isolates. Folia Microbiol. 49, 75–78 (2004).CrossRefGoogle Scholar
  22. Shintani H.: Modification of medical device surface to attain anti-infection. Trends Biomater.Artif.Organs 18, 1–8 (2004).Google Scholar
  23. Singh P., Cameotra S.S.: Potential applications of microbial surfactants in biomedical sciences. Trends Microbiol. 22, 142–146 (2004).Google Scholar
  24. Trebichavský I., Šplíchal I.: Probiotics manipulate host cytokine response and induce antimicrobial peptides. Folia Microbiol. 51, 507–510 (2004).CrossRefGoogle Scholar
  25. Velraeds M.W., Van Der Mei H.C., Reid G., Busscher H.J.: Inhibition of initial adhesion of uropathogenic Enterococcus faecalis by biosurfactants from Lactobacillus isolates. Appl.Eviron.Microbiol. 62, 1958–1963 (1996).Google Scholar
  26. Velraeds M.W., Van De Belt-Gritter B., Van Der Mei H.C., Reid G., Busscher H.J.: Interference in initial adhesion of uropathogenic bacteria and yeasts to silicone rubber by a Lactobacillus acidophilus biosurfactant. J.Med.Microbiol. 47, 1081–1085 (1998).PubMedGoogle Scholar
  27. Vesterlund S., Karp M., Salminen S., Ouwehand A.C.: Staphylococus aureus adheres to human intestinal mucus but can be displaced by certain lactic acid bacteria. Microbiology 152, 1819–1826 (2006).PubMedCrossRefGoogle Scholar
  28. Walencka E., Sadowska B., Różalska S., Hryniewicz W., Różalska B.: Staphylococcus aureus biofilm as a target for single or repeated doses of oxacillin, vancomycin, linezolid and/or lysostaphin. Folia Microbiol. 51, 381–386 (2006).CrossRefGoogle Scholar

Copyright information

© Institute of Microbiology, v.v.i, Academy of Sciences of the Czech Republic 2008

Authors and Affiliations

  • E. Walencka
    • 1
  • S. Różalska
    • 2
  • B. Sadowska
    • 1
  • B. Różalska
    • 1
    Email author
  1. 1.Department of Immunology and Infectious Biology, Institute of Microbiology and ImmunologyUniversity of ŁódźŁódźPoland
  2. 2.Department of Industrial Microbiology and Biotechnology, Institute of Microbiology and ImmunologyUniversity of ŁódźŁódźPoland

Personalised recommendations