Antisolvent Crystallization of L-histidine in Micro-Channel Reactor under Microgravity
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The influence of gravity (including 0 G, 0.16 G, 0.38 G, 1 G and 1.8 G) on the antisolvent crystallization of L-histidine was investigated in a self-designed micro-channel crystallizer on a zero-G flight, which was successfully launched on June 13th, 2018. The stable form of L-histidine was obtained under microgravity, while only the metastable form can be observed in the ground experiments with the same inlet conditions. The possible reason is the extremely ordered flow field of the system in microgravity environment. A large amount of small particles aggregated at 1.8 G due to the enhanced micromixing, which favors nucleation. Bigger particles crystalized at Moon gravity (0.16 G) without the excessive consumption of the supersaturation by explosive nucleation and with moderate convection of the system. It is concluded that both nucleation and crystal growth were influenced by the micromixing status in the system due to the altered gravity extent.
KeywordsL-histidine Microgravity Antisolvent crystallization Polymorphism Micro-channel crystallizer
The authors thank Spacety Co., Ltd. (in China) for the support of microgravity experiments. This work is founded by National Natural Science Foundation of China (Grants 21878294, 21776284, 21606234), Major National Scientific Instrument Development Project (21427814), Key Research Program of Frontier Sciences, CAS (QYZDJ-SSW-JSC030) and Instrument Developing Project of Chinese Academy of Sciences (YZ201641).
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