Microgravity Science and Technology

, Volume 30, Issue 6, pp 839–847 | Cite as

Photoperiod-controlling Guttation and Growth of Rice Seedlings Under Microgravity on Board Chinese Spacelab TG-2

  • Lihua Wang
  • Fei Han
  • Hui Qiong ZhengEmail author
Original Article
Part of the following topical collections:
  1. Approaching the Chinese Space Station - Microgravity Research in China


Guttation has been shown to play a crucial role in controlling plant growth and development by involvement of the transport of water, but how does this water transport in plant from roots to leaves work against pull of gravity is still unknown. The aim of the present study was to evaluate the influence of microgravity on photoperiod-controlling guttation and growth of rice (Oryza sativa L.) seedlings on board the Chinese Spacelab TongGong-2(TG-2). The growth rate of rice seedlings was closely correlated with guttation under both the microgravity and the ground conditions, which was increased by microgravity under both a 16-h long-day (LD) and an 8-h short-day (SD) photoperiod conditions. In addition, guttation of the TG-2 grown rice under the LD condition was more significant in comparison with that under the SD condition. These results indicated that microgravity affected the photoperiod-controlling growth of rice seedlings could be related to the enhanced guttation in space.


Rice Microgravity Photoperiod Guttation Chinese space lab 



The authors are indebted to Prof. Tao Zhang’s group in Shanghai Institute of Technique Physics for PCB construction and Prof. Weining Sun for helping in the space experiment. This work was supported by the China Manned Space Flight Technology project TG-2 and the National Natural Science Foundation of China (31670864), the National natural fund joint fund project(U1738106), the Strategic Pioneer Projects of CAS (XDA15013900) and the National Science Foundation for Young Scientists of China (31500687).

Supplementary material

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  1. Bailey, K.J., Leegood, R.C.: Nitrogen recycling from the xylem in rice leaves: dependence upon metabolism and associated changes in xylem hydraulics. J. Exp. Bot. 67, 2901–2911 (2016)CrossRefGoogle Scholar
  2. Brown, A.H., Chapman, D.K., Heathcote, D.G.: Characterization of precocious seedling development observed during IML-1 mission. ASGSB Bullet. 6, 58 (1992)Google Scholar
  3. Bubenheim, D.L., Stieber, J., Campbell, W.F., Salisbury, F.B., Levinski, M., Sytchev, V., Pdolsky, I., Chernova, L.: Induced abnormality in Mir- and earth grown super dwarf wheat. Adv. Space Res. 31, 229 (2003)CrossRefGoogle Scholar
  4. Chen, Y.C., Lin, T.C., Martin, C.E.: Effects of guttation prevention on photosynthesis and transpiration in leaves of Alchemilla mollis. Photosynthetica 52, 371–376 (2014)CrossRefGoogle Scholar
  5. Cochard, H., Venisse, J.S., Barigah, T.S., Brunel, N., Herbette, S., Guilliot, A., Tyree, M.T., Sakr, S.: Putative role of aquaporins in variable hydraulic conductance of leaves in response to light. Plant Physiol. 143, 122–133 (2007)CrossRefGoogle Scholar
  6. De Micco, V., Arena, C., Pignalosa, D., Durante, M.: Review. Effects of sparsely and densely ionizing radiation on plants. Rad. Environ. Biophys. 50, 1 (2011)CrossRefGoogle Scholar
  7. Engel, H., Friederichsen, U.I.: Periodische guttation bei Zea mays. Planta 44, 459–471 (1954)CrossRefGoogle Scholar
  8. Hoson, T., Soga, K., Mori, R., Saiki, M., Wakabayashi, K., Kamisaka, S., Kamigaichi, S., Aizawa, S., Yoshizaki, I., Mukai, C., Shimazu, T., Fukui, K., Yamashita, M.: Morphogenesis of rice and Arabidopsis seedlings in space. J. Plant Res. 112, 477–486 (1999)CrossRefGoogle Scholar
  9. Huisinga, B.: Influence of light on growth, geotropism and guttation of Avena seedlings grown in total darkness. Plant Biol. 13, 445–487 (1964)Google Scholar
  10. Kim, Y.X., Steudle, E.: Gating of aqùaporins by light and reactive oxygen species in leaf parenchyma cells of the midrib of Zea mays. J. Exp. Bot. 60, 547–556 (2009)CrossRefGoogle Scholar
  11. Kuang, A., Xia, Y., Mcclure, G., Musgrave, M.E.: Influence of microgravity on ultrastructure and storage reserves in seeds of Brssica rapa L. Ann. Bot. 85, 851–859 (2000)CrossRefGoogle Scholar
  12. Lersten, L.R., Curtis, J.D.: Hydathodes in physocarpus (rosaceae: Spiraeoideae). Can. J. Bot. 60, 850–855 (1982)CrossRefGoogle Scholar
  13. Link, B.M., Busse, J.S., Stankovic, B.: Seed-to-seed-to-seed growth and development of Arabidopsis in microgravity. Astrobiology 14, 866–874 (2014)CrossRefGoogle Scholar
  14. Luo, X., Zheng, J., Huang, R., Huang, Y., Wang, H., Jiang, L., Fang, X.: Phytohormones signaling and crosstalk regulating leaf angle in rice. Plant Cell Rep. 35, 2423–2433 (2016)CrossRefGoogle Scholar
  15. Maeda, E., Maeda, K.: Ultrastructural studies of leaf hydathodes. I. Wheat (Triticum aestivum) leaf tips. Jpn. J. Crop Sci. 56, 641–651 (1987)Google Scholar
  16. Maeda, E., Maeda, K.: Ultrastructural studies of leaf hydathodes II. Rice (Oryza sativa) leaf tips. Jpn. J. Crop Sci. 57, 733–742 (1988)CrossRefGoogle Scholar
  17. Mcintyre, G.I.: The role of transpiration in phototropism of the Avena coleoptile: evidence of stomatal control of the phototropic response. Australian J. Plant Physiol. 21, 359–375 (1994)MathSciNetGoogle Scholar
  18. Millar, K.D., Kumar, P., Correll, M.J., Mullen, J.L., Hangarter, R.P., Edelmann, R.E., Kiss, J.Z.: A novel phototropic response to red light is revealed in microgravity. New Phytol. 186, 648–656 (2010)CrossRefGoogle Scholar
  19. Miyamoto, K., Hoshino, T., Yamashita, M., Ueda, J.: Automorphosis of etiolated pea seedlings in space is simulated by a three-dimensionsl clinostat and the application of inhibitors of auxin polar transport. Physiol. Plant. 123, 467–474 (2005)CrossRefGoogle Scholar
  20. Morohashi, K., Okamoto, M., Yamazaki, C., Fujii, N.: Gravitropism interferes with hydrotropism via counteracting auxin dynamics in cucumber roots: clinorotation and spaceflight experiments. New Phytol. 215, 1476–1489 (2017)CrossRefGoogle Scholar
  21. Musgrave, M.E., Kuang, A., Matthews, S.W.: Plant reproduction during spaceflight: importance of the gaseous environment. Planta 203(Suppl.), 177–184 (1997)CrossRefGoogle Scholar
  22. Pedersen, O.: Long-distance water transport in aquatic plants. Plant Physiol. 103, 1369–1375 (1993)CrossRefGoogle Scholar
  23. Schwenke, H., Wagner, E.: A new concept of root exudation. Plant Cell Environ. 15, 289–299 (1992)CrossRefGoogle Scholar
  24. Singh, S.: Guttation: quantification, microbiology and implications for phytopathogy. In: Luttege, U., Beyschlag, W., Cushman, J. (eds.) Progress in Botany, vol. 75, pp 187–214. Springer, Berlin (2014)Google Scholar
  25. Singh, S.: Guttation: mechanism, momentum and modulation. Bot. Rev. 82, 149–182 (2016)CrossRefGoogle Scholar
  26. Singh, S., Singh, T.N., Chauhan, J.S.: Guttation in rice: occurrence, regulation and significance in varietal improvement. J. Crop Improv. 23, 351–365 (2009)CrossRefGoogle Scholar
  27. Singh, S., Singh, T.N.: Guttation 1: chemistry, crop husbandry and molecular farming. Phytochem. Rev. 12, 147–172 (2013)CrossRefGoogle Scholar
  28. Vandenbrink, J.P., Kiss, J.Z., Herranz, R., Medina, F.J.: Light and gravity signals synergize in modulating plant development. Fron. Plant Sci. 5, 563 (2014)Google Scholar
  29. Vandenbrink, J.P., Herranz, R., Medina, F.J., Edelmann, R.E., Kiss, J.Z.: A novel blue-light phototropic response is revealed in roots of Arabidopsis thaliana in microgravity. Planta 244, 1201–1215 (2016)CrossRefGoogle Scholar
  30. Walter, H.U.: In: Hu, W (ed.) Fluid Sciences and Materials Science in Space: a European Perspective. Springer, Berlin (1987)Google Scholar
  31. Wegner, L.H.: Root pressure and beyond: energetically uphill water transport into xylem vessels. J. Exp. Bot. 65, 381–393 (2014)CrossRefGoogle Scholar
  32. Wolffet, S.A., Coelho, L.H., Zabrodina, M., Brinckmann, E., Kittan, A.-I.: Plant mineral nutrition, gas exchange and photosynthesis in space: a review. Adv. Space Res. 51, 465–475 (2013)CrossRefGoogle Scholar
  33. Zhang, Y., Wang, L., Xie, J., Zheng, H.Q.: Differential protein expression profiling of Arabidopsis thaliana callus under microgravity on board the Chinese SZ-8 spacecraft. Planta 241, 475–488 (2015)CrossRefGoogle Scholar
  34. Zheng, H.Q.: Flowering in space. Micrograv. Sci. Technol (2018)Google Scholar

Copyright information

© Springer Nature B.V. 2018
corrected publication 2018

Authors and Affiliations

  1. 1.CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and EcologyChinese Academy of SciencesShanghaiChina

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