Drosophila GENE Experiment in the Spanish Soyuz Mission to the ISS: II. Effects of the Containment Constraints

  • Raúl Herranz
  • David A. Laván
  • F. Javier Medina
  • Jack J. W. A. van Loon
  • Roberto Marco
Open Access
Original Article

Abstract

In the GENE experiment performed during an 11-day Soyuz Mission to the International Space Station (ISS), we intended to determine if microgravity affects Drosophila metamorphosis processes. Control experiments were performed including a 1g ground control parallel to the ISS flight samples and a Random Position Machine microgravity simulated control. A preliminary analysis of the results indicates that five hundred to one thousand genes change their expression profiles depending on the cut-off levels selected. Especially affected among them are the mitochondrial ones (an example with the respiratory chain is presented). We show here that there is a synergic effect of the constraints introduced to meet the requirements of the space experiment (mainly, a cold step and the use of hermetically closed Type-I containers). The cold transport step to the launch site was introduced to slow down the pupal development. The hermetically closed Type I containers were required to ensure the containment of the fixative (acetone) in the experiment. As shown here, the oxygen concentration inside the container was not optimal but fully compatible with pupal development. It is highly likely that such combined environmental effects will become a common finding in these types of studies as they become more complicated and extensive. They could open the way to understand how the gene expression patterns and the actual phenotypes can adjust to the environment. These findings indicate the importance of a vigorous ground based program in support of real microgravity experiments. Only then we can utilize the ISS in order to understand the consequences of the modified environment in outer space on living organisms.

Keywords

Microgravity Drosophila RPM Random position machine ISS International Space Station Systems biology Pupation Affymetrix microarray Gene expression profile Space biological experiments constrains Oxygen limitation Redundancy and robustness 

Supplementary material

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Supplementary Figure (DOC 32 kb)
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Supplementary Fig. S2(JPEG 2.06 Mb)

References

  1. Adams, et al.: The genome sequence of Drosophila melanogaster. Science. 287(5461), 2185–95 (2000)CrossRefGoogle Scholar
  2. Biorack on D1: ESA SP-1091. In: Longdon, N., David, V. (eds.) ESA/ESTEC. Noordwijk, the Netherlands (1988) FebruaryGoogle Scholar
  3. Dalma-Weiszhausz, D.D., Warrington, J., Tanimoto, E.Y., Miyada, C.G.: The affymetrix GeneChip platform: an overview. Methods Enzymol. 410, 3–28 (2006)CrossRefGoogle Scholar
  4. Gene Spring GX 7.3: Expression analysis. http://www.agilent.com/chem/genespring (2007)
  5. Girardot, F., Monnier, V., Tricoire, H.: Genome wide analysis of common and specific stress responses in adult Drosophila melanogaster. BMC Genomics 5(1), 74 (2004)CrossRefGoogle Scholar
  6. Herranz, R., Husson, D., Villa, A., Pastor, M., Medina, F.J., Marco, R.: Modifications in basic handling techniques to study the consequences of the Drosophila melanogaster exposure to the Space environment. J. Gravit. Physiol. 12(2), 51–60 (2005a)Google Scholar
  7. Herranz, R., Benguria, A., Fernández-Pineda, E., Medina, F.J., Gasset, G., van Loon, J.J., Zaballos, A., Marco, R.: Gene expression variations during Drosophila metamorphosis in Space. The GENE Experiment in the Spanish Cervantes Mission to the ISS. J Gravit Physiol. 12(1), 253–254 (2005b)Google Scholar
  8. Herranz, R., Laván, D.A., Benguría, A., Duque, P., Leandro, L.J., Gasset, G., Medina, F.J., van Loon, J., Marco, R.: The “GENE” Experiment in the Spanish Soyuz Mission to the International Space Station. Effects of cold transportation. Microgravity Sci. Technol. 19(2), 45–49 (2007)Google Scholar
  9. Jensen, D., Overgaard, J., Srensen, J.G.: The influence of developmental stage on cold shock resistance and ability to cold-harden in Drosophila melanogaster. J. Insect Phys. 53, 179–186 (2007)CrossRefGoogle Scholar
  10. Leandro, L.J., Szewczyk, N.J., Benguría, A., Herranz, R., Laván, D., Medina, F.J., Gasset, G., van Loon, J., Conley, C.A., Marco, R.: Comparative analysis of Drosophila melanogaster and Caenorhabditis elegans gene expression experiments in the European Soyuz Flights to the International Space Station. Adv Space Res. 40(4), 506–512 (2007)CrossRefGoogle Scholar
  11. Lee, R.E., Denlinger, D.L.: Insects at Low Temperature. Chapman and Hall, NY (1991)Google Scholar
  12. Qin, W., Neal, S.J., Robertson, R.M., Westwood, J.T., Walker, V.K.: Cold hardening and transcriptional change in Drosophila melanogaster. Insect Mol. Biol. 14(6), 607–613 (2005) DecCrossRefGoogle Scholar
  13. van Loon, J.J.W.A.: Some history and use of the random positioning machine, RPM, in gravity related research. Adv. Space Res. 39, 1161–1165 (2007)CrossRefGoogle Scholar
  14. van Loon, J.J.W.A., Medina, F.J., Stenuit, H., Istasse, E., Heppener, M., Marco, R.: The National–ESA Soyuz missions Andromède, Marco Polo, Odissea, Cervantes, DELTA and Eneide. Microgravity Sci. Technol. 19(5/6), 9–32 (2007)CrossRefGoogle Scholar

Copyright information

© The Author(s) 2008

Authors and Affiliations

  • Raúl Herranz
    • 1
    • 2
  • David A. Laván
    • 1
  • F. Javier Medina
    • 3
  • Jack J. W. A. van Loon
    • 4
  • Roberto Marco
    • 1
  1. 1.Departamento de Bioquímica-I.I.Biomédicas “Alberto Sols” (UAM-CSIC)MadridSpain
  2. 2.Universidad Autonoma de Madrid Facultad de Medicina, Arzobispo MorcilloMadridSpain
  3. 3.Centro de Investigaciones Biológicas (CSIC)MadridSpain
  4. 4.Dutch Experiment Support CenterDESC @ OCB-ACTA-Vrije UniversiteitAmsterdamThe Netherlands

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