Rendiconti Lincei

, Volume 28, Issue 1, pp 1–9 | Cite as

Meta-cleavage pathway of phenol degradation by Acinetobacter sp. strain AQ5NOL 1

  • Siti Aqlima Ahmad
  • Nor Aripin Shamaan
  • Mohd Arif Syed
  • Ariff Khalid
  • Nor Arina Ab Rahman
  • Khalilah Abdul Khalil
  • Farrah Aini Dahalan
  • Mohd Yunus Shukor
Article

Abstract

The characterization of bacterial enzymatic pathways of phenol metabolism is important to better understand phenol biodegradation. Phenol hydroxylase is the first enzyme involved in the oxidative metabolism of phenol, followed by further degradation via either meta- or ortho-pathways. In this study, the first known instance of phenol degradation via the meta-pathway by a member of the genus Acinetobacter (Acinetobacter sp. strain AQ5NOL 1) is reported. Phenol hydroxylase converts phenol to catechol, which is then converted via the meta-pathway to 2-hydroxymuconic semialdehyde by the catechol 2,3-dioxygenase enzyme. Phenol hydroxylase extracted from strain AQ5NOL 1 was fully purified using DEAE-Sepharose®, DEAE-Sephadex®, Q-Sepharose® and Zorbax® Bioseries GF-250 gel filtration and was demonstrated by SDS-PAGE to have a molecular weight of 50 kDa. The phenol hydroxylase was purified to about 210.51 fold. The optimum pH and temperature for enzyme activities are 20 °C and 7–7.5, respectively. The apparent K m and V max values of phenol hydroxylase with phenol as the substrate were 13.4 µM and 2.5 µmol min−1 mg−1, respectively. The enzyme was stable at −20 °C for 36 days.

Keywords

Purification Characterization Phenol hydroxylase Acinetobacter sp. 

Notes

Acknowledgments

This work was supported by the Research Grant Scheme (RUGS) 2009, Universiti Putra Malaysia (Vote No. 91851).

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Copyright information

© Accademia Nazionale dei Lincei 2016

Authors and Affiliations

  • Siti Aqlima Ahmad
    • 1
  • Nor Aripin Shamaan
    • 2
  • Mohd Arif Syed
    • 1
  • Ariff Khalid
    • 3
  • Nor Arina Ab Rahman
    • 1
  • Khalilah Abdul Khalil
    • 4
  • Farrah Aini Dahalan
    • 5
  • Mohd Yunus Shukor
    • 1
  1. 1.Department of Biochemistry, Faculty of Biotechnology and Biomolecular SciencesUniversiti Putra MalaysiaSerdangMalaysia
  2. 2.Faculty of Medicine and Health SciencesUniversiti Sains Islam MalaysiaKuala LumpurMalaysia
  3. 3.Faculty of Health SciencesUniversiti Kebangsaan MalaysiaKuala LumpurMalaysia
  4. 4.The School of Environmental EngineeringUniversiti Malaysia PerlisArauMalaysia
  5. 5.Biomolecular Science Program, School of Biology, Faculty of Applied SciencesUniversiti Teknologi MARAShah AlamMalaysia

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