Rendiconti Lincei

, Volume 27, Issue 4, pp 653–663 | Cite as

Modelling the kinetics of hexavalent molybdenum (Mo6+) reduction by the Serratia sp. strain MIE2 in batch culture

  • Mohd Izuan Effendi Halmi
  • Siti Rozaimah Sheikh Abdullah
  • Wan Lutfi Wan Johari
  • Mohd Shukuri Muhammad Ali
  • Noor Azmi Shaharuddin
  • Ariff Khalid
  • Mohd Yunus Shukor
Article
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Abstract

In the present work, the kinetics of hexavalent molybdenum reduction by the Serratia sp. strain MIE2 were investigated using several kinetic models, such as Monod, Haldane, Teissier, Aiba, Yano, Han and Levenspiel and Luong. The statistical analysis showed that the best model was Teissier, which had the lowest RMSE and AICc values, the highest adjusted R 2 values, and an F test and with a bias factor and an accuracy factor nearest to unity (1.0). The calculated value for the Teissier constants, such as p max, K s and K i , was 0.506 µmol Mo-blue h−1, 6.53 mM and 29.41 mM, respectively. The effect of heavy metals showed that hexavalent molybdenum reduction by the strain MIE2 was inhibited by silver, mercury and copper with a total inhibition of 96, 97, and 45 %, respectively, at a concentration of 1 ppm. Otherwise, the Mo-reducing enzyme was inhibited by mercury and zinc with an inhibition of 88 and 65 %, respectively. Most of the respiratory inhibitors did not inhibit the Mo-reducing enzyme activity, indicating that the respiratory system in this bacterium is not the site of the hexavalent molybdenum reduction. The results obtained from this study could be useful for estimating the relationship between molybdenum-blue production and the molybdate concentration, which may be important during the up scaling of the molybdenum bioremediation process.

Keywords

Bioremediation Molybdenum Modelling kinetics Heavy metals Respiratory inhibitors 
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Notes

Acknowledgments

This project was supported by funds from the Ministry of Science, Technology and Innovation (MOSTI) under the Science Fund Grant nos. 02-01-04-SF0445 and 02-01-04-SF0445.

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Copyright information

© Accademia Nazionale dei Lincei 2016

Authors and Affiliations

  • Mohd Izuan Effendi Halmi
    • 1
    • 3
  • Siti Rozaimah Sheikh Abdullah
    • 3
  • Wan Lutfi Wan Johari
    • 2
  • Mohd Shukuri Muhammad Ali
    • 1
  • Noor Azmi Shaharuddin
    • 1
  • Ariff Khalid
    • 4
  • Mohd Yunus Shukor
    • 1
  1. 1.Department of Biochemistry, Faculty of Biotechnology and Biomolecular SciencesUniversiti Putra Malaysia (UPM)SerdangMalaysia
  2. 2.Department of Environment, Faculty of Environmental StudiesUniversiti Putra Malaysia (UPM)SerdangMalaysia
  3. 3.Department of Chemical Engineering and Process, Faculty of Engineering and Built EnvironmentUniversiti Kebangsaan Malaysia (UKM)BangiMalaysia
  4. 4.Department of Biomedical Sciences Programme, Faculty of Health SciencesUKMKuala LumpurMalaysia

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