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Rendiconti Lincei

, Volume 27, Issue 4, pp 597–602 | Cite as

Karyological characteristics of the Koh Tao caecilian, Ichthyophis kohtaoensis (Amphibia: Gymnophiona: Ichthyophiidae) by conventional staining and Ag-NOR banding techniques

  • Isara Patawang
  • Krit Pinthong
  • Sumalee Phimphan
  • Yodchaiy Chuaynkern
  • Prateep Duengkae
  • Alongklod TanomtongEmail author
Article
  • 205 Downloads

Abstract

The karyotypic analysis of the Koh Tao caecilian, Ichthyophis kohtaoensis Taylor, on 1960 from Surin Province, Thailand was obtained from the present study. Mitotic chromosome was prepared directly from the small intestine of specimens after in vivo colchicine treatment. The metaphase spreads were performed on microscopic slides and air-dried. Conventional staining, and Ag-NOR banding techniques were applied to stain the chromosome with Giemsa’s solution. Results showed that the number of diploid chromosome was 2n = 42, while the fundamental number (NF) was 64 in both males and females. The type of chromosomes included 6 large metacentric, 2 large submetacentric, 12 small metacentric, 2 small submetacentric, and 20 small telocentric chromosomes. We found that nucleolar organizer regions/NORs (the representative of chromosome marker) were located on the small telocentric chromosome pair 19. No cytologically distinguishable sex chromosome was observed. The karyotype formula is as follows: 2n (42) = L 6 m  + L 2 sm  + S 12 m  + S 2 sm  + S 20 t .

Keywords

Ichthyophis kohtaoensis Karyotype Chromosome 

Notes

Acknowledgments

This work was supported by the Science Achievement Scholarship of Thailand (SAST) and Toxic Substances in Livestock and Aquatic Animals Research Group, Khon Kaen University. Our specials thank goes to Siriporn Ghai for her assistance in correcting the manuscript.

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Copyright information

© Accademia Nazionale dei Lincei 2016

Authors and Affiliations

  • Isara Patawang
    • 1
  • Krit Pinthong
    • 2
  • Sumalee Phimphan
    • 1
  • Yodchaiy Chuaynkern
    • 1
  • Prateep Duengkae
    • 3
  • Alongklod Tanomtong
    • 1
    Email author
  1. 1.Toxic Substances in Livestock and Aquatic Animals Research Group, Department of Biology, Faculty of ScienceKhon Kaen UniversityKhon KaenThailand
  2. 2.Department of Fundamental Science, Faculty of Science and TechnologySurindra Rajabhat UniversitySurinThailand
  3. 3.Department of Forest Biology, Faculty of ForestryKasetsart UniversityBangkokThailand

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