Effect of forest bathing trips on human immune function
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In Japan, a forest bathing trip, called “Shinrinyoku” in Japanese, is a short, leisurely visit to a forest; it is regarded as being similar to natural aromatherapy. This review focuses on the effects of forest bathing trips on human immune function. Beginning in 2005, adult Japanese individuals, both male and female, participated in a series of studies aimed at investigating the effect of forest bathing trips on human immune function. The subjects experienced a 3-day/2-night trip to forest areas, and blood and urine were sampled on days 2 (the first sampling during each trip) and 3 (the second sampling during each trip), and on days 7 and 30 after the trips. Natural killer (NK) activity, the numbers of NK, granulysin-, perforin-, and granzymes A/B-expressing lymphocytes in the blood, and the concentration of urinary adrenaline were measured. The same measurements were made before the trips on a normal working day as a control. The mean values of NK activity and the numbers of NK, granulysin-, perforin-, and granzymes A/B-expressing cells on forest bathing days were significantly higher than those on the control days, whereas the mean values of the concentration of urinary adrenaline on forest bathing days were significantly lower than that on the control days in both male and female subjects. The increased NK activity lasted for more than 30 days after the trip, suggesting that a forest bathing trip once a month would enable individuals to maintain a higher level of NK activity. In contrast, a visit to the city as a tourist did not increase NK activity, the numbers of NK cells, or the level of intracellular granulysin, perforin, and granzymes A/B. These findings indicate that forest bathing trips resulted in an increase in NK activity, which was mediated by increases in the number of NK cells and the levels of intracellular granulysin, perforin, and granzymes A/B.
KeywordsForest bathing Granulysin Granzyme NK activity Perforin
What is a forest bathing trip?
The forest environment has been enjoyed by humans for a long time because of the quiet atmosphere, beautiful scenery, mild climate, and clean fresh air in forests. In Japan, a forest bathing trip, called “Shinrinyoku” in Japanese, is a short, leisurely visit to a forest and is regarded as being similar to natural aromatherapy. A forest bathing trip involves visiting a forest for relaxation and recreation while breathing in volatile substances, called phytoncides (wood essential oils), which are antimicrobial volatile organic compounds derived from trees, such as α-pinene and limonene [1, 2, 3, 4]. Incorporating forest bathing trips into a good lifestyle was first proposed in 1982 by the Forest Agency of Japan. It has now become a recognized relaxation and/or stress management activity in Japan [2, 3, 4, 5, 6]. The results of a study using the Profile of Mood States (POMS) test demonstrated that a forest bathing trip significantly increased the score for vigor and decreased the scores for anxiety, depression, and anger [2, 4]. Habitual forest bathing may help to decrease the risk of psychosocial stress-related diseases . Because forests occupy 67% of the land in Japan , forest bathing is easily accessible, and according to a public opinion poll conducted in Japan in 2003, 25.6% of respondents had participated in a forest bathing trip, indicating its popularity in Japan . Moreover, forest bathing is possible in similar environments throughout the world.
Effect of phytoncides (essential oils) from trees on human immune function
To investigate the effect of a forest bathing trip on human immune function, the effects of phytoncides on human natural killer (NK) activity and intracellular levels of perforin, granzyme A (GrA), and granulysin (GRN) in NK cells were studied in vitro. NK cells have been reported to kill tumors or virus-infected cells through the release of perforin, granzymes, and GRN via the granule exocytosis pathway [8, 9, 10, 11, 12]. Li et al.  incubated NK-92MI cells, an interleukin-2-independent human NK cell line, in the presence of phytoncides, such as α-pinene, 1,8-cineole, d-limonene, and essential oils extracted from trees including Japanese cedar (Cryptomeria, Sugi in Japanese) and Chamaecyparis obtuse (Hinoki in Japanese), for 48–144 h and then measured NK activity and the intracellular levels of perforin, GrA, and GRN . They found that phytoncides significantly increased the cytolytic activity of NK-92MI cells in a dose-dependent manner and significantly increased the intracellular levels of perforin, GrA, and GRN in NK-92MI cells. Phytoncides also partially, but significantly, restored the decreased human NK activity and the decreased perforin, GrA, and GRN levels in NK-92MI cells induced by dichlovos, an organophosphorus pesticide. Pretreatment with phytoncides partially prevented dichlovos-induced inhibition of NK activity. Taken together, these data indicate that phytoncides significantly enhance human NK activity and that this effect is at least partially mediated by the induction of intracellular perforin, GrA, and GRN . Komori et al.  reported that the citrus fragrance found in forests affected human endocrine and immune systems, based on the measurement of urinary cortisol and dopamine levels, NK activity, and CD4/8 ratios. da Silva et al.  found that volatile oil from Zanthoxylum rhoifolium Lam leaves and certain terpenes (α-humulene, β-caryophyllene, α-pinene, and β-pinene) exhibited anti-tumor efficacy and significant immunomodulatory action in vivo and in vitro in mice. Moreover, Grassmann et al.  found that the essential oil from Pinus mugo showed antioxidative properties. All of these findings strongly suggest that forest environments (forest bathing trip) have beneficial effects on human immune function. However, to date, there have been no published reports on the effect of forest bathing on human immune function, with the exception of the studies conducted by this author.
Effect of forest bathing trips on human immune function
Within the framework of published results on forest bathing trips, since 2005, a series of investigations with both Japanese female and male subjects have been carried out with the aim of studying the effect of forest bathing on human immune function. To this end, investigators measured human NK activity and the numbers/proportions of NK and T cells, perforin-, GrA/B-, and GRN-expressing lymphocytes in human peripheral blood lymphocytes (PBLs) in the subjects during visits to forest areas in Japan [2, 3, 4].
In the first study , 12 healthy male subjects, aged 37–55 years (43.1 ± 6.1 years), were selected from three large companies in Tokyo, Japan. The sociodemographic information on the subjects, including age and lifestyle habits, was obtained by means of a self-administered questionnaire and has been reported previously . None of the subjects had any signs or symptoms of infectious diseases, used drugs that might affect immunological analysis, or were taking any medication at the time of the study. It was also confirmed that none of the subjects had taken forest bathing trips within at least 3 months prior to the study. In early September 2005, the subjects participated in a 3-day/2-night trip to forest areas at Iiyama, Nagano prefecture, located in northwest Japan. On day 1, the subjects walked about 2.5 km in a forest field in about 2 h, which approximates the amount of normal physical activity the subjects would have on an average working day. The participants were allowed to rest anywhere and anytime they liked. On day 2, they walked about 2.5 km in the morning and afternoon (each time approx. 2 h), respectively, in two different forest fields. On day 3, the subjects finished the trip, and after providing blood samples and completing a questionnaire survey, they returned to Tokyo. The main trees in the forests were Japanese cedar (Cryptomeria), Japanese beech, and Japanese oak. Blood samples were taken on the second (the first sampling during the trip) and third days (the second sampling during the trip). White blood cell (WBC) counts, NK activity, numbers of NK and T cells, and numbers of GRN-, perforin-, and GrA/B-expressing lymphocytes were determined in the blood samples. The same measurements were made before the trips on a normal working day as a control. Blood was sampled at 0800 hours on all occasions. To control for the effect of alcohol on NK activity, the subjects did not consume alcohol for 2 days before providing the blood samples. The subjects did not take hot spring baths or eat any special foods, such as herbs and alcoholic drinks, which may have affected the immune function during the trip. Phytoncide concentrations in forest air samples were also measured.
How long does the increased NK activity last after a forest bathing trip?
Following the first study, two questions remained to be resolved: (1) will a trip to places without forest (a city tourist visit) also increase NK activity? (2) How long does the increased NK activity last after a forest bathing trip or a city tourist visit? Two investigations were then conducted to address these two questions . Twelve healthy male subjects, aged 35–56 years (mean 45.1 ± 6.7 years), were selected from four large companies in Tokyo, Japan. Information on the subjects was gathered from a self-administered questionnaire, including age and lifestyle habits, as described previously . None of the subjects had any signs or symptoms of infectious disease, used drugs that may affect the immunological analysis, or were taking any medication at the time of the study. It was also confirmed that none of the subjects had taken forest bathing trips within at least 3 months prior to the study. In early September 2006, the subjects experienced a 3-day/2-night trip to three different Chamaecyparis obtuse (Japanese cypress, Hinoki in Japanese) forests around Agematsu town, Nagano prefecture, located in northwest Japan. The schedule of the forest bathing trip was similar to that described previously . Prior to the forest visit, in mid-May 2006, 11 of the same 12 subjects participated in a city tourist visit consisting of 3-day/2-night trip to Nagoya city [2, 3]. On the first day, the subjects walked for 2 h in the afternoon along a tourist route through a historical district of Nagoya city and then stayed at a hotel in Nagoya. On the second day, the subjects walked for 2 h around Nagoya baseball Dome in the morning and 2 hours around/in Nagoya airport nearby Nagoya city in the afternoon. There are some tree-covered areas in Nagoya city, but there are almost no trees in the areas visited. The class of hotel and the lifestyle of the subjects during the stays in the hotels were the same for the city and forest trips. The walking courses in both trips were 2.5 km, as in the previous study . Blood was sampled at 0800 hours on the second (the first sampling during each trip) and third days (the second sampling during each trip), on days 7 and 30 after the forest bathing trip, and 3 days prior to the trips (control). The WBC counts, NK activity, proportions of NK and T cells, and GRN-, perforin-, and GrA/B-expressing cells in PBLs were measured. The concentration of adrenaline in urine was also determined. To control for the effect of alcohol on NK activity, the subjects did not consume alcohol for 2 days prior to blood sampling, including before and during the trips, and after the trip on days 7 and 30. The purpose of setting the control experiment in a city in this study was to determine whether just taking a trip (city tourist visit) can also affect NK activity.
Effect of forest bathing trips on NK activity in female subjects 
Although these studies demonstrated that forest bathing trips were able to enhance human NK activity in male subjects, the question remaining to be resolved was whether or not forest bathing trips would also increase NK activity in female subjects. It has been reported that menstrual cycle significantly affects NK activity ; consequently, the influence of the menstrual cycle on NK activity should be controlled for in experiments with female subjects.
In this study , 13 healthy nurses, aged 25–43 years (mean 28.8 ± 4.6 years) and active in their profession for 4–18 years (mean 6.7 ± 3.8), were selected with informed consent. None of the subjects had any signs or symptoms of infectious disease, used drugs that may affect immunological analysis, or were taking any medication at the time of the study. The subjects experienced a 3-day/2-night trip to forest fields around Shinano town, Nagano prefecture, located in northwest Japan in early September 2007. The schedule of the forest bathing trip and blood sampling was similar to that described for the earlier studies [2, 3], and the same blood/urine tests were carried out (WBC counts, NK activity, numbers of NK and T cells, and GRN-, perforin-, and GrA/B-expressing lymphocytes in the blood samples; concentrations of adrenaline and noradrenaline in urine) as well as tests measuring the concentrations of estradiol and progesterone in serum. The same control measurements were made before the trip on a normal working day. Blood was sampled at 0800 hours on all days. The concentrations of phytoncides in the forests were measured.
Souza et al. reported that NK activity is significantly higher in the follicular phase of the menstrual cycle than in the luteal phase and that the levels of NK activity in postmenopausal women are similar to those of women in the follicular phase but significantly higher than those of women in the luteal phase . Conversely, Yovel et al.  reported that the menstrual cycle had no significant effect on the activity levels of NK cells. Roszkowski et al.  found that patients with low (<50 pg/ml) and high (>200 pg/ml) estradiol levels showed an increase and a decrease of NK cell activity, respectively. Progesterone at 100–400 nM (31.45–125.8 ng/ml) inhibits NK activity in healthy pregnant women, whereas 100-fold higher concentrations are required for reducing NK activity in non-pregnant women . These suggest that the menstrual cycle and the levels of estradiol and progesterone in serum may affect human NK activity.
To control for the influence of menstrual cycle on NK activity, a questionnaire was administered to obtain information on the menstrual cycle of the subjects. The ratios of subjects who were in the follicular phase during the experiment were 5/13, 6/13, 6/13, 7/13, and 6/13 on the day before the trip, days 1 and 2 during the trip, and days 7 and 30 after the trip, respectively, indicating that there was no significant difference in the proportion of women in different phases of the menstrual cycle between the different days. This observation suggests that the menstrual cycle had a similar influence on the average of NK activity on the different days. Therefore, statistical analyses to compare the mean values of NK activity on the different days by a paired t test should be appropriate. In addition, the concentrations of estradiol and progesterone in the serum of the subjects were also measured to confirm the influence of estradiol and progesterone on NK activity. In this study, there was no significant difference in the concentration of estradiol in serum between the days before, during, and after the forest bathing trip, indicating that, in this case, estradiol had a similar effect in all subjects on NK activity on the different days. Although the levels of progesterone on days 1 and 2 were higher than that before the study, the difference in serum progesterone concentration between days 1 or 2 and before the trip was not significant, suggesting that progesterone had a similar effect on NK activity on the different days .
Many factors, including circadian variation , physical exercise [16, 24], and alcohol consumption [16, 25], can affect human NK activity. In order to control for the effect of circadian rhythm on NK activity, blood was sampled at 0800 hours on all days [2, 3, 4]. To control for the effect of physical exercise on NK activity, the number of steps taken during the walking trips were limited to average normal workday distances, as monitored by a pedometer. The levels of physical activity among all trips were also matched. To control for the effect of alcohol on NK activity, the subjects did not consume alcohol for 2 days before providing the blood sample during the study period for both trips, including before the trips and after the trips on days 7 and 30. The number of hours spent sleeping during the trips were slightly more than those on average working days; however, the difference was not significant in either type of trip. Kusaka et al.  reported that the number of sleeping hours did not affect NK activity or NK cell numbers under physiological conditions. Li et al.  also found that there was no difference in the number of NK cells or the levels of perforin-, GRN-, or GrA/B-expressing cells in PBL among subjects who slept for 5, 6, or 7 h, respectively. In addition, although the number of hours slept during the city tourist visit were slightly more than those on an average working days, the NK activities during the trip were almost the same as for working days, indicating that the longer sleeping hours did not affect NK activity in the city tourist visit . Taken together, it can be concluded that although the number of sleeping hours during the trips was slightly higher than those on average working days, this difference did not affect NK activity or cell numbers in the city tourist trip.
Effect of forest bathing trips on stress hormones
Because NK cells can kill tumor cells by releasing anti-cancer proteins, such as perforin, GRN, and GrA/B, and forest bathing trips increase NK activity and the intracellular level of anti-cancer proteins, the above findings suggest that forest bathing trips may have a preventive effect on cancer generation and development.
Parts of the experimental data shown in this review and performed by author were supported by a grant from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (No. 16107007). This project is a collaboration with Professor Yoshifumi Miyazaki at Chiba University, Professor Kanehisa Morimoto at Osaka University, Mr. Takahide Kagawa, Forestry and Forest Products Research Institute, and Dr. Tomoyuki Kawada, Department of Hygiene and Public Health, Nippon Medical School (Professor and Chief). The author is grateful to the staff of Department of Hygiene and Public Health, Nippon Medical School for their technical assistance in the experiments.
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