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ROS and NO Dynamics in Endothelial Cells Exposed to Exercise-Induced Wall Shear Stress

  • Yan-Xia Wang
  • Hai-Bin Liu
  • Peng-Song Li
  • Wen-Xue Yuan
  • Bo Liu
  • Shu-Tian Liu
  • Kai-Rong Qin
Article
  • 24 Downloads

Abstract

Introduction

Intracellular reactive oxygen species (ROS) and nitric oxide (NO) levels are associated with vascular homeostasis and diseases. Exercise can modulate ROS and NO production through increasing frequency and magnitude of wall shear stress (WSS). However, the details of ROS and NO production in endothelial cells and their interplay under WSS induced by exercise at different intensities remain unclear.

Methods

In this study, we developed an in vitro multicomponent nonrectangular flow chamber system to simulate pulsatile WSS waveforms induced by moderate and high intensity exercise. Furthermore, the dynamic responses of ROS and NO in endothelial cells and the relationship between ROS and NO were investigated under the WSS induced by different intensity exercise.

Results

After exposing to WSS induced by moderate intensity exercise, endothelial cells produced more NO than those under high intensity exercise-induced WSS. In this process, ROS was found to play a dual role in the generation of intracellular NO. Under WSS induced by moderate intensity exercise, modest elevated ROS promoted NO production, whereas excessive ROS in endothelial cells exposed to WSS induced by high intensity exercise attenuated NO bioavailability. Interestingly, antioxidant N-acetylcysteine (NAC) could increase NO production under WSS induced by high intensity exercise.

Conclusions

Our results provide some cues for selecting appropriate exercise intensities and elevating benefits of exercise on endothelial function. Additionally, owing to the consistency of our results and some in vivo phenomena, this flow chamber system may serve as an in vitro exercise model of arterial vessel for future studies.

Keywords

Exercise Wall shear stress (WSS) Reactive oxygen species (ROS) Nitric oxide (NO) Endothelial cells 

Notes

Acknowledgments

The research described in this paper was supported in part by the National Natural Science Foundation of China (Grant Nos. 31370948, 11672065) and the Fundamental Research Funds for the Central Universities in China (Grant No. DUT18JC15).We would like to thank Prof. Wenyu Liu for kindly revising the manuscript.

Conflict of Interest

Yan-Xia Wang, Hai-Bin Liu, Peng-Song Li, Wen-Xue Yuan, Bo Liu, Shu-Tian Liu, Kai-Rong Qin declare no conflicts of interest.

Ethical Approval

All human subjects research was carried out in accordance with the Helsinki Declaration of 1975, as revised in 2000 (5) and approved by the Ethics Committee of Dalian University of Technology. No animal studies were carried out by the authors for this article.

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Copyright information

© Biomedical Engineering Society 2018

Authors and Affiliations

  1. 1.Department of Engineering Mechanics, State Key Laboratory of Structural Analysis for Industrial EquipmentDalian University of TechnologyDalianChina
  2. 2.School of Optoelectronic Engineering and Instrumentation ScienceDalian University of TechnologyDalianChina
  3. 3.Department of Physical EducationDalian University of TechnologyDalianChina
  4. 4.School of Biomedical EngineeringDalian University of TechnologyDalianChina

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