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Cellular and Molecular Bioengineering

, Volume 8, Issue 1, pp 106–118 | Cite as

Impulsive Enzymes: A New Force in Mechanobiology

  • Peter J. ButlerEmail author
  • Krishna K. Dey
  • Ayusman SenEmail author
Article

Abstract

We review studies that quantify newly discovered forces from single enzymatic reactions. These forces arise from the conversion of chemical energy to kinetic energy, which can be harnessed to direct diffusion of the enzyme up a concentration gradient of substrate, a novel phenomenon of molecular chemotaxis. When immobilized, enzymes can move fluid around them and perform directional pumping in microfluidic chambers. Because of the extensive array of enzymes in biological cells, we also develop three new hypotheses: that enzymatic self diffusion can assist in organizing signaling pathways in cells, can assist in pumping of fluid in cells, and can impose biologically significant forces on organelles, which will be manifested as stochastic motion not explained by thermal forces or myosin II. Such mechanochemical phenomena open up new directions in research in mechanobiology in which all enzymes, in addition to their primary function as catalysts for reactions, may have secondary functions as initiators of mechanosensitive transduction pathways.

Keywords

Cell mechanics Mechanobiology Myosin Phosphorylation Rheology Diffusiophoresis Molecular swimmer Chemotaxis Cytoplasm 

Notes

Acknowledgments

PJB acknowledges financial support from the National Science Foundation. CMMI-1334847. AS acknowledges financial support from the Penn State Center for Nanoscale Science (NSF-MRSEC, DMR-0820404).

Conflict of interest

Peter J. Butler, Krishna K. Dey, and Ayusman Sen have no conflicts of interest to declare.

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Copyright information

© Biomedical Engineering Society 2015

Authors and Affiliations

  1. 1.Department of Biomedical EngineeringThe Pennsylvania State UniversityUniversity ParkUSA
  2. 2.Department of ChemistryThe Pennsylvania State UniversityUniversity ParkUSA

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