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Cell Stress and Chaperones

, Volume 24, Issue 6, pp 1137–1149 | Cite as

Identification of an Apis cerana cerana MAP kinase phosphatase 3 gene (AccMKP3) in response to environmental stress

  • Yuzhen Chao
  • Chen Wang
  • Haihong Jia
  • Na Zhai
  • Hongfang Wang
  • Baohua Xu
  • Han LiEmail author
  • Xingqi GuoEmail author
Original Paper

Abstract

MAP kinase phosphatase 3 (MKP3), a member of the dual-specificity protein phosphatase (DUSP) superfamily, has been widely studied for its role in development, cancer, and environmental stress in many organisms. However, the functions of MKP3 in various insects have not been well studied, including honeybees. In this study, we isolated an MKP3 gene from Apis cerana cerana and explored the role of this gene in the resistance to oxidation. We found that AccMKP3 is highly conserved in different species and shares the closest evolutionary relationship with AmMKP3. We determined the expression patterns of AccMKP3 under various stresses. qRT-PCR results showed that AccMKP3 was highly expressed during the pupal stages and in adult muscles. We further found that AccMKP3 was induced in all the stress treatments. Moreover, we discovered that the enzymatic activities of peroxidase, superoxide dismutase, and catalase increased and that the expression levels of several antioxidant genes were affected after AccMKP3 was knocked down. Collectively, these results suggest that AccMKP3 may be associated with antioxidant processes involved in response to various environmental stresses.

Keywords

MAP kinase phosphatase 3 Oxidative stress RNA interference Environmental stresses Apis cerana cerana 

Notes

Funding information

This work was financially supported by Funds of the National Natural Science Foundation of China (No. 31572470) and the Earmarked Fund for the China Agriculture Research System (No. CARS-44).

Supplementary material

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Supplementary Fig. 1

(PNG 98.8 kb)

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High Resolution Image (TIF 574 kb)

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Copyright information

© Cell Stress Society International 2019

Authors and Affiliations

  1. 1.State Key Laboratory of Crop Biology, College of Life SciencesShandong Agricultural UniversityTai’anPeople’s Republic of China
  2. 2.College of Animal Science and TechnologyShandong Agricultural UniversityTai’anPeople’s Republic of China

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