RNA-Seq analysis of Polyrhachis vicina Roger and insights into the heat shock protein 90 and 70 families
- 67 Downloads
The heat shock protein 90 (Hsp90) and heat shock cognate proteins (Hsc70) have been identified as chaperones of the ecdysone receptor (EcR)/ultraspiracle protein (USP) heterocomplex. However, little is known about the status of Hsp90 and Hsc70 in Polyrhachis vicina Roger. Here, we sequenced the transcriptomes of adult ants in P. vicina for the first time. Clean reads in female, male, and worker ants were annotated into 40,147 transcripts, and 37,488, 28,300, and 33,638 unigenes were assembled in female, male, and worker ants, respectively. According to RPKM, the numbers of differentially expressed genes between female and male ants, between female and worker ants, and between male and worker ants and the common differentially expressed genes were 12,657, 21,630, 15,112 and 3704, respectively. These results reveal that caste differentiation, caste specificity formation, and social divisions of P. vicina ants may be due to gene expression differences. Moreover, PvEcR and PvUSP were also detected as differentially expressed genes in the ants; specifically, PvUSP expression was higher than PvEcR expression in all castes. We speculate that PvUSP may have a role similar to that of juvenile hormone receptor. Four identified PvHsp90 family members and 23 identified PvHsp70 family members were found in the ants, and 2 PvHsp90 genes and 8 PvHsp70 genes were analyzed by qRT-PCR. Among those genes, the expression of 2 PvHsp90 genes and 5 PvHsp70 genes coincided with the expression profiles of PvEcR and PvUSP, which suggest that the characterization of PvHsp90 and PvHsc70 may be as EcR/USP molecular chaperones in P. vicina.
KeywordsPolyrhachis vicina Roger Transcriptome Heat shock protein 90 family Heat shock protein 70 family Differentially expressed genes
We thank Wang X and Liu C for comments on this manuscript.
This work was supported by grants from the National Natural Science Foundation of China (Nos. 31571276 and 31171195).
- Cao J, Liu Y, Yang Y, Zhang H, Li Z, Yang Q, Zhang S, Zhang Q, Liu X (2015) Molecular characterization and functional analysis of the ultraspiracle (USP) in the oriental fruit moth Grapholita molesta (Lepidoptera: Olethreutidae). Comp Biochem Physiol B Biochem Mol Biol 190:54–62. https://doi.org/10.1016/j.cbpb.2015.08.008 CrossRefGoogle Scholar
- Grabherr MG, Haas BJ, Yassour M, Levin JZ, Thompson DA, Amit I, Adiconis X, Fan L, Raychowdhury R, Zeng Q, Chen Z, Mauceli E, Hacohen N, Gnirke A, Rhind N, di Palma F, Birren BW, Nusbaum C, Lindblad-Toh K, Friedman N, Regev A (2011) Full-length transcriptome assembly from RNA-Seq data without a reference genome. Nat Biotechnol 29:644–652. https://doi.org/10.1038/nbt.1883 CrossRefGoogle Scholar
- Planello R, Herrero O, Gomez-Sande P, Ozaez I, Cobo F, Servia MJ (2015) Ecdysone-related biomarkers of toxicity in the model organism Chironomus riparius: stage and sex-dependent variations in gene expression profiles. PLoS One 10:e0140239. https://doi.org/10.1371/journal.pone.0140239 CrossRefGoogle Scholar
- Prentice HM, Milton SL, Scheurle D, Lutz PL (2004) The upregulation of cognate and inducible heat shock proteins in the anoxic turtle brain. J Cereb Blood Flow Metab 24:826–828. https://doi.org/10.1097/01.WCB.0000126565.27130.79 CrossRefGoogle Scholar
- Tsan MF, Gao B (2004) Heat shock protein and innate immunity. Cell Mol Immunol 1:274–279Google Scholar
- Wang H, Li K, Zhu JY, Fang Q, Ye GY, Wang H, Li K, Zhu JY (2012) Cloning and expression pattern of heat shock protein genes from the endoparasitoid wasp, Pteromalus puparum in response to environmental stresses. Arch Insect Biochem Physiol 79:247–263. https://doi.org/10.1002/arch.21013 CrossRefGoogle Scholar
- Yan T, Chen H, Sun Y, Yu X, Xia L (2016) RNA interference of the ecdysone receptor genes EcR and USP in grain aphid (Sitobion avenae F.) affects its survival and fecundity upon feeding on wheat plants. Int J Mol Sci 17. https://doi.org/10.3390/ijms17122098
- Zou C et al (2017) Cucurbitacin B acts a potential insect growth regulator by antagonizing 20-hydroxyecdysone activity. Pest Manag Sci. https://doi.org/10.1002/ps.4817