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MiR-1246 is upregulated and regulates lung cell apoptosis during heat stress in feedlot cattle

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Cell Stress and Chaperones Aims and scope

Abstract

Globally, heat stress seriously threatens productivity of cattle. The objective of this study was to identify novel miRNAs that regulated heat stress in feedlot cattle. Experiment was conducted under heat stress and normal conditions. With profiling miRNAs of each feedlot cattle, our results showed the level of miR-1246 was significantly increased in these heat-stressed cattle (P < 0.05). Furthermore, by using bioinformatics analysis and luciferase reporter assays combined with qPCR and western blot, we found miR-1246 negatively regulated poly (C) binding protein 2 (PCBP2) and cAMP response element binding protein-like 2 (CREBL2) mRNA and protein levels through binding to the 3′-UTR region (P < 0.05); further, it inhibited heat-induced apoptosis in lung cells. Finally, our results suggested that miR-1246 plays an important role in heat stress and it has the potential to be a novel modulation factor for heat stress in feedlot cattle.

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Funding

This work was financially supported by the Chongqing Municipal Science and Technology Commission project (no. cstc2017jcyjBX0013) The Research and Innovation Project of Chongqing Graduate Students ( no.CYB18074).

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Authors and Affiliations

  1. Chongqing Key Laboratory of Forage & Herbivore, College of Animal Science, Southwest University, Chongqing, 402460, People’s Republic of China

    Yu Hu, Ling Wang, Zhong-Gang Luo, Gong-Wei Zhang & Fu-Yuan Zuo

  2. Institute of Animal Genetics and Breeding, Sichuan Agricultural University, Chengdu, 611130, Sichuan, People’s Republic of China

    Ming-Chen Cai

  3. Key Laboratory of Pig Industry Sciences, Ministry of Agriculture, Chongqing Academy of Animal Sciences, Chongqing, 402460, People’s Republic of China

    Ting-Huan Zhang

Authors
  1. Yu Hu
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  2. Ming-Chen Cai
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  3. Ling Wang
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  4. Ting-Huan Zhang
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  5. Zhong-Gang Luo
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  6. Gong-Wei Zhang
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  7. Fu-Yuan Zuo
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Contributions

Y.H. and M.C.C. designed and performed the main experiments, Y.H. interpreted the results and wrote the manuscript. M.C.C. performed animal experiment; they equally contributed to this work. L.W. performed the qRT-PCR. T.H.Z. performed the vector construction. Z.G.L. performed the GO biological process enrichment and KEGG pathway network analyses. G.W.Z. amended the manuscript. F.Y.Z. designed the experiments and supervised the research. All authors read the final manuscript.

Corresponding author

Correspondence to Fu-Yuan Zuo.

Ethics declarations

All animal procedures were approved by the Southwest University Institutional Animal Care and Use Committee.

Competing interests

The authors declare that they have no competing interests.

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Hu, Y., Cai, MC., Wang, L. et al. MiR-1246 is upregulated and regulates lung cell apoptosis during heat stress in feedlot cattle. Cell Stress and Chaperones 23, 1219–1228 (2018). https://doi.org/10.1007/s12192-018-0927-9

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  • DOI: https://doi.org/10.1007/s12192-018-0927-9

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