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Cell Stress and Chaperones

, Volume 23, Issue 5, pp 1069–1078 | Cite as

Protective effects of zymosan on heat stress-induced immunosuppression and apoptosis in dairy cows and peripheral blood mononuclear cells

  • Yuhang Sun
  • Jin Liu
  • Gengping Ye
  • Fang Gan
  • Mohammed Hamid
  • Shengfa Liao
  • Kehe Huang
Original Paper

Abstract

Dairy cows exposed to heat stress (HS) show decreased performance and immunity, but increased heat shock protein expressions and apoptosis. Zymosan, an extract from yeast cell walls, has been shown to modulate immune responses and defense against oxidative stress. However, few literatures are available about the effects of zymosan on immune responses and other parameters of the dairy cows under HS. Here, both primary peripheral blood mononuclear cell (PBMC) and dairy cow models were established to assess the effects of zymosan on performance, immunity, heat shock protein, and apoptosis-related gene expressions of dairy cows under HS. In vitro study showed that proliferation, IL-2 production, and Bcl-2/Bax-α ratio of cow primary PBMC were reduced, whereas hsp70 mRNA and protein expressions, as well as Annexin V-bing, were increased when PBMCs were exposed to heat. In contrast, zymosan significantly reversed these above changes induced by the HS. In the in vivo study, 40 Holstein dairy cows were randomly selected and assigned into zymosan group (supplemental zymosan; n = 20) and control group (no supplemental zymosan; n = 20). The results showed that zymosan improved significantly the dry matter intake and milk yield, increased IgA, IL-2, and tumor necrosis factor-α (TNF-α) contents in sera, as well as hepatic Bcl-2/Bax-α ratio, but decreased respiration rate and hepatic hsp70 expressions in the dairy cows under HS. Taken together, zymosan could alleviate HS-induced immunosuppression and apoptosis and improve significantly the productive performance and immunity of dairy cows under HS.

Keywords

Zymosan Heat stress Dairy cows Peripheral blood mononuclear cells Immunity Apoptosis 

Notes

Funding information

This work was supported by the National Key R & D Program (2016YFD0501203), the National Natural Science Foundation of China (No. 31472253 and 31772811), and the Priority Academic Program Development of Jiangsu Higher Education Institutions (Jiangsu, China).

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflict of interests.

Supplementary material

12192_2018_916_Fig6_ESM.png (960 kb)
Fig. S1

Daily air temperature in the barn during this study. This study was performed for 28 days, during which time the air temperature was recorded 3 times daily at 0700, 1400 and 2100 h, respectively. (PNG 31 kb)

12192_2018_916_MOESM1_ESM.tiff (162 kb)
High Resolution (TIFF 161 kb)

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Copyright information

© Cell Stress Society International 2018

Authors and Affiliations

  1. 1.College of Veterinary MedicineNanjing Agricultural UniversityNanjingChina
  2. 2.Shanghai Bright Holstein Co., Ltd.ShanghaiChina
  3. 3.Department of Animal and Dairy SciencesMississippi State UniversityStarkvilleUSA

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