Genome-wide microRNA profiling of bovine milk-derived exosomes infected with Staphylococcus aureus
Bovine milk is rich in exosomes, which contain abundant miRNAs and play important roles in the regulation of neonatal growth and development of adaptive immunity. Here, we analyzed miRNA expression profiles of bovine milk exosomes from three healthy and three mastitic cows, and then six miRNA libraries were constructed. Interestingly, we detected no scRNAs and few snRNAs in milk exosomes; this result indicated a potential preference for RNA packaging in milk exosomes. A total of 492 known and 980 novel exosomal miRNAs were detected, and the 10 most expressed miRNAs in the six samples accounted for 80–90% of total miRNA-associated reads. Expression analyses identified 18 miRNAs with significantly different expression between healthy and infected animals; the predicted target genes of differentially expressed miRNAs were significantly enriched in immune system process, response to stimulus, growth, etc. Moreover, target genes were significantly enriched in several Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways including inflammatory, immune, and cancer pathways. Our survey provided comprehensive information about milk exosomes and exosomal miRNAs involved in mastitis. Moreover, the differentially expressed miRNAs, especially miR-223 and miR-142-5p, could be considered as potential candidates for mastitis.
KeywordsBovine Milk Exosome Mastitis miRNA-sequencing
SJL and MCC contributed to concept design. HBH and WDX performed the animal experiments. MCC, BWL, and YS performed the RNA and protein isolation and western blot. MCC, HBH, and XBJ performed data analysis. MCC, SYC, and JW prepared the manuscript. All authors read and approved the final manuscript.
This research was supported by the Science and Technology Support Program in Sichuan (No. 2014NZ0032-A).
Compliance with ethical standards
All experiments were carried out according to the Regulations for the Administration of Affairs Concerning Experimental Animals published by the Ministry of Science and Technology, China in 2004 and approved by the Institutional Animal Care and Use Committee of Sichuan Agricultural University, China.
Conflict of interest
The authors declare that they have no conflict of interest.
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