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Molecular cloning and transcriptional regulation of Indian peafowl (Pavo cristatus) IFN-α gene

  • Yu Wang
  • Hongjing Zhao
  • Juanjuan Liu
  • Yizhi Shao
  • Mingwei XingEmail author
Original Paper
  • 13 Downloads

Abstract

Interferon-α (IFN-α) resists viral infections by triggering the transcription of a diverse range of antiviral IFN-stimulated genes (ISGs). However, information about the Indian peafowl (Pavo cristatus) IFN-α (PcIFN-α) has not been reported. In this study, a PcIFN-α gene was amplified, which encoded a protein of 193 amino acids with a 26-amino acid signal peptide sharing 72.16–95.70% identity with other avians in Aves. After expression in prokaryote, PcIFN-α was analyzed for its physicochemical property and antiviral activity. Intriguingly, compared with chicken IFN-α, an effective viral infection therapeutic agent, PcIFN-α showed superior anti-VSV, NDV, and AIV activities, which were then abrogated by rabbit anti-PcIFN-α antibodies in vitro. Moreover, PcIFN-α was shown to be highly sensitive to trypsin; however, it remained stable despite changes in pH and temperature. Additionally, PcIFN-α induced the transcriptional or translational levels of Mx1 and ISG12 genes time-dependently. Overall, the present study revealed that PcIFN-α is a potential novel effective therapeutic agent in antiviral defense responses in peafowl, improving understanding of its involvement in bird antiviral defense.

Keywords

Pavo cristatus Interferon-α Molecular cloning Characterization Interferon-stimulated genes 

Notes

Acknowledgements

We thank Dr. Qu Liandong and Dr. Wang Xiurong from the Harbin Veterinary Research Institute for assistance with VSV, NDV, and AIV.

Funding information

This work was supported by the National Key Research and Development Program of China (Grant No.2017YFD0501702), the Fundamental Research Funds for the Central Universities (Grant No. 2572016EAJ5), and the National Natural Science Foundation of China (Grant No.31672619).

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Copyright information

© Cell Stress Society International 2019

Authors and Affiliations

  • Yu Wang
    • 1
  • Hongjing Zhao
    • 1
  • Juanjuan Liu
    • 1
  • Yizhi Shao
    • 1
  • Mingwei Xing
    • 1
    Email author
  1. 1.College of Wildlife ResourcesNortheast Forestry UniversityHarbinChina

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